Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2018  |  Volume : 14  |  Issue : 57  |  Page : 352-357

Anti-inflammatory properties of Bixa Orellana leaves extract are associated with suppression of bradykinin-induced endothelial hyperpermeability

1 Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia
2 Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia

Correspondence Address:
Yoke Keong Yong
Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_540_17

Rights and Permissions

Background: Previous study showed that an aqueous extract of Bixa orellana L. (Family of Bixaceae) leaf (AEBO) is capable of inhibiting bradykinin (BK)-induced inflammation in animal models. Objective: This study further investigates the effect of AEBO on BK-induce inflammation in vitro model. Materials and Methods: The endothelial barrier protective effect of AEBO was examined via an in vitro endothelial permeability assay. Human umbilical vein endothelial cell (HUVEC) was first pretreated with AEBO with a concentration range from 0.1, 0.2, and 0.4 mg/mL and then induced with BK. Fluorescein isothiocyanate-conjugated-dextran was used as an indicator of permeability flux. To elucidate its mechanism of action, the phospholipase C (PLC) – nitric oxide (NO) – cyclic guanosine monophosphate (cGMP) signaling pathway and protein kinase C (PKC) activity were evaluated. Results: Pretreatment of AEBO significantly (P < 0.05) suppressed BK-induced HUVEC hyperpermeability and 0.4 mg/mL possessed the maximal inhibitory effect (87%, 70%, and 57% inhibition rate at 5, 15, and 30 min time point, respectively). Moreover, AEBO has presented remarkable IC50 = 0.24 mg/mL for anti-PLC activity, 0.36 mg/mL for anti-NO production, and 0.19 mg/mL for anti-cGMP production. For PKC inhibition, the IC50 (0.42 mg/mL) was slightly higher compared to others. Conclusion: This study provided supportive evidence for the previous study where AEBO exhibited anti-inflammatory activity against BK in vivo. The anti-inflammatory activity of AEBO may partly be associated with the reduction of endothelial hyperpermeability via the suppression of PLC-NO-cGMP signaling and PKC activity. Abbreviations used: AEBO: Aqueous extract of Bixa orellana; BK: Bradykinin; PLC: Phospholipase C; NO: Nitric oxide; cGMP: Cyclic guanosine monophosphate; PKC: Protein kinase C; HUVEC: Human umbilical vein endothelial cell; PBS: Phosphate buffered saline; eNOS: Endothelial nitric oxide synthase.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded185    
    Comments [Add]    
    Cited by others 2    

Recommend this journal