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ORIGINAL ARTICLE
Year : 2018  |  Volume : 14  |  Issue : 56  |  Page : 444-450

Bioactivity-guided isolation of antioxidant compounds from Pouzolzia zeylanica (L.) benn


1 Department of Pharmaceutical Analysis, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China
2 Department of Pharmaceutical Analysis, School of Pharmacy, Chongqing University, Chongqing, China

Correspondence Address:
Lujun Wang
Department of Pharmaceutical Analysis, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000
China
Zhining Xia
Department of Pharmaceutical analysis, School of Pharmacy, Chongqing University, Chongqing, 401331
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_233_17

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Background: Pouzolzia zeylanica (L.) Benn. (Family: Urticaceae), which is widely distributed in China, is consumed as food and as a traditional herbal medicine for the treatment of numerous diseases. Objective: The aim of the present study was to explore the antioxidant compounds of P. zeylanica. Materials and Methods: A petroleum ether extract, an ethyl acetate extract (EAE), an n-butanol extract, and the remaining part were separated by liquid—liquid extraction from a 90% aqueous ethanol extract and a water extract of P. zeylanica and were evaluated using two complementary antioxidant assays, namely 2,2-diphenyl-1-picrylhydrazyl-free radical scavenging and ferric-reducing antioxidant power assays. Results: The EAE, which possessed the highest antioxidant activity, contained the highest total phenolic content (263.5 ± 4.8 mg/g) and total flavonoid content (388.3 ± 5.6 mg/g). In addition, three compounds with antioxidant activity, quercetin, kaempferol, and N-[2-(3-hydroxy-4-methoxyphenyl)-2-hydroxyethyl]-3-(4-methoxyphenyl) prop-2-enamide, were isolated from the EAE. Conclusions: These results demonstrate that the flavonoids and phenolic compounds from P. zeylanica could be used as natural antioxidants. Abbreviations used: P. zeylanica: Pouzolzia zeylanica (L.) Benn., PE: Petroleum ether extract, EAE: Ethyl acetate extract, BE: n-butanol extract, RE: Remaining part, WE: Water extract, DPPH: 2,2-Diphenyl-1-picrylhydrazyl, FRAP: Ferric-reducing antioxidant power, TPC: Total phenolic content, TFC: Total flavonoid content, BHA: Butylated hydroxyanisole, BHT: Butylated hydroxytoluene, TPTZ: 2,4,6-Tris (2-pyridyl)-s-triazine, HPLC: High-performance liquid chromatography, CC: Column chromatography.


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