| ORIGINAL ARTICLE |
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| Year : 2018 | Volume
: 14
| Issue : 56 | Page : 444-450 |
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Bioactivity-guided isolation of antioxidant compounds from Pouzolzia zeylanica (L.) benn
Lujun Wang1, Die Gao1, Qifeng Fu1, Kai Zhou2, Zhining Xia2
1 Department of Pharmaceutical Analysis, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China
2 Department of Pharmaceutical Analysis, School of Pharmacy, Chongqing University, Chongqing, China
Correspondence Address:
Lujun Wang
Department of Pharmaceutical Analysis, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000
China
Zhining Xia
Department of Pharmaceutical analysis, School of Pharmacy, Chongqing University, Chongqing, 401331
China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/pm.pm_233_17
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Background: Pouzolzia zeylanica (L.) Benn. (Family: Urticaceae), which is widely distributed in China, is consumed as food and as a traditional herbal medicine for the treatment of numerous diseases. Objective: The aim of the present study was to explore the antioxidant compounds of P. zeylanica. Materials and Methods: A petroleum ether extract, an ethyl acetate extract (EAE), an n-butanol extract, and the remaining part were separated by liquid—liquid extraction from a 90% aqueous ethanol extract and a water extract of P. zeylanica and were evaluated using two complementary antioxidant assays, namely 2,2-diphenyl-1-picrylhydrazyl-free radical scavenging and ferric-reducing antioxidant power assays. Results: The EAE, which possessed the highest antioxidant activity, contained the highest total phenolic content (263.5 ± 4.8 mg/g) and total flavonoid content (388.3 ± 5.6 mg/g). In addition, three compounds with antioxidant activity, quercetin, kaempferol, and N-[2-(3-hydroxy-4-methoxyphenyl)-2-hydroxyethyl]-3-(4-methoxyphenyl) prop-2-enamide, were isolated from the EAE. Conclusions: These results demonstrate that the flavonoids and phenolic compounds from P. zeylanica could be used as natural antioxidants.
Abbreviations used: P. zeylanica: Pouzolzia zeylanica (L.) Benn., PE: Petroleum ether extract, EAE: Ethyl acetate extract, BE: n-butanol extract, RE: Remaining part, WE: Water extract, DPPH: 2,2-Diphenyl-1-picrylhydrazyl, FRAP: Ferric-reducing antioxidant power, TPC: Total phenolic content, TFC: Total flavonoid content, BHA: Butylated hydroxyanisole, BHT: Butylated hydroxytoluene, TPTZ: 2,4,6-Tris (2-pyridyl)-s-triazine, HPLC: High-performance liquid chromatography, CC: Column chromatography.
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