| ORIGINAL ARTICLE |
|
| Year : 2018 | Volume
: 14
| Issue : 56 | Page : 364-370 |
|
|
Ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry analysis of In vitro metabolites of lignans from fructus forsythiae by Human Fecal Flora
Shihua Xing, Chongsheng Peng, Mengyue Wang, Ying Peng, Xiaobo Li
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China
Correspondence Address:
Xiaobo Li
School of Pharmacy, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Minhang, Shanghai 200240
China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/pm.pm_177_17
|
|
|
Background: Phillyrin and (+)-pinoresinol 4-O-β-D-glucoside are the major active furofuran-type lignans in Fructus Forsythiae. The metabolic routes and metabolites of these two lignans are not well understood yet. Objective: In this study, we attempted to identify the human-intestine bacterial metabolites of lignans from Fructus Forsythiae. Materials and Methods: Two natural compounds, phillyrin and (+)-pinoresinol 4-O-β-D-glucoside were incubated with human fecal microflora in an anaerobic incubator for 72 h and the metabolites with highly sensitive ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) were analyzed. Results: As a result, nine metabolites were determined using a Syncronis™ C18column (particle size 1.7 mm) in a gradient elution system. These metabolites were then identified according to the mass fragmental mechanism, MS/MS fragment ions, and previous publications. The results of this study indicated that the major metabolites of furofuran-type lignans are through the processes of hydrolysis, demethylation, reduction, dehydroxylation, and oxidation. Conclusions: Lignans can be metabolized by intestinal microbiota and the intestinal bacteria play a critical role in the metabolism of components administered orally.
Abbreviations used: GAM: General anaerobic medium; UPLC/Q-TOF-MS: Ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry; ESI: Negative ion electrospray; RT: Retention time; TCM: Traditional Chinese Medicine.
|
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
