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Year : 2018  |  Volume : 14  |  Issue : 56  |  Page : 322-327

Ultrasonication extraction, bioactivity, antioxidant activity, total flavonoid, total phenolic and antioxidant of Clitoria Ternatea linn flower extract for anti-aging drinks

Faculty of Public Health, Mahasarakham University, Maha Sarakham 44150, Thailand

Correspondence Address:
Buavaroon Srichaikul
Faculty of Public Health, Mahasarakham University, Maha Sarakham 44150
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_206_17

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Background: Anti-aging health drinks currently are one of the largest consumer drinks. People are more concerned about their health and quality of health life in order to increase longevity. We have investigated that many anti-aging health drink products in Thailand are developed below the standard and do not meet the Thai FDA requirements in terms of bioactivities and toxicity analysis. Objective: This study aimed to evaluate the bioactivity quantities, active ingredients, total phenolic contents, and different extraction methods essential to obtain definite value of bioactivities, quantified antioxidant activity, and also acute toxicity analysis of Clitoria ternatea Linn flower extract drink. Materials and Methods: We performed ultrasonication extraction and maceration with 40% and 50% ethyl alcohol in different periods of time and also analysis of antioxidant activities with 2,2-diphenyl-1-picrylhydrazyl, ferric-reducing antioxidant power, and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid methods, total phenolic compound, active in gradients by high-performance liquid chromatography (HPLC). Results: We found that C. ternatea Linn flower extract drink consisted of high contents of gallic acid and rutin by HPLC method. There was no acute toxicity of C. ternatea Linn flower extract health drink. Abbreviations used: DPPH: 2,2-diphenyl-1-picrylhydrazyl; FRAP: Ferric-reducing antioxidant power; ABTS: 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid; HPLC: High-performance liquid chromatography; EtOH: Ethanol; PTZ: (2,4, 6—tripyridyl—striazine); CH3OH: Acetic acid; CH3OH: Methyl alcohol.

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