| ORIGINAL ARTICLE |
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| Year : 2017 | Volume
: 13
| Issue : 51 | Page : 512-516 |
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Anti-epileptic effects of valepotriate isolated from Valeriana jatamansi jones and its possible mechanisms
An Wu1, Xia Ye2, Qiang Huang2, Wei-Min Dai2, Jian-Min Zhang1
1 Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou 310009, P.R. China
2 Department of Neurosurgery, People's Hospital of Quzhou City, Quzhou 324000, P.R. China
Correspondence Address:
Jian-Min Zhang
Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, No. 88 Jiefang Road, Hangzhou 310009
P.R. China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0973-1296.211027
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Objective: This work aimed to investigate the anti-epileptic effects of valepotriate isolated from Valeriana jatamansi Jones and studied its possible mechanisms. Methods: The anti-epileptic effects of valepotriate were studied using maximal electroshock-induced seizure (MES), pentylenetetrazole (PTZ)-induced epilepsy, and pentobarbital sodium-induced sleeping model in mice. The possible anti-epileptic mechanisms of valepotriate were investigated by analyzing the expressions of GABAA, GABAB, glutamic acid decarboxylase (GAD65), Bcl-2, and caspase-3 in the brain using Western blot assay. Results: The results indicated that valepotriate showed significant anti-epileptic activity against MES- and PTZ-induced epilepsy at doses of 5, 10, and 20 mg/kg, and ED50values for MES- and PTZ-induced epilepsy were 7.84 and 7.19 mg/kg, respectively. Furthermore, valepotriate (10 and 20 mg/kg) can significantly prolong sleeping time and shorten the latency time on the pentobarbital sodium-induced sleeping time test. Furthermore, valepotriate (5, 10, and 20 mg/kg) could significantly up-regulate the expression of GABAA, GAD65, and Bcl-2 and down-regulate the expression of caspase-3, but had no significant effect on the expression of GABAB. Conclusion: The results indicated that valepotriate had anti-epileptic activity and the mechanisms might be associated with regulation of GABA and inhibition of neuronal apoptosis. |
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