Chemical composition of Moringa oleifera ethyl acetate fraction and its biological activity in diabetic human dermal fibroblasts
Sivapragasam Gothai1, Katyakyini Muniandy1, Mazni Abu Zarin1, Tan Woan Sean1, S Suresh Kumar2, Murugan A Munusamy3, Sharida Fakurazi4, Palanisamy Arulselvan1
1 Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
2 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
3 Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia
4 Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia; Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
|Date of Submission||12-Aug-2016|
|Date of Acceptance||08-Nov-2016|
|Date of Web Publication||11-Oct-2017|
Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Moringa oleifera (MO), commonly known as the drumstick tree, is used in folklore medicine for the treatment of skin disease. Objective: The objective of this study is to evaluate the ethyl acetate (EtOAc) fraction of MO leaves for in vitro antibacterial, antioxidant, and wound healing activities and conduct gas chromatography-mass spectrometry (GC-MS) analysis. Materials and Methods: Antibacterial activity was evaluated against six Gram-positive bacteria and 10 Gram-negative bacteria by disc diffusion method. Free radical scavenging activity was assessed by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radical hydrogen peroxide scavenging and total phenolic content (TPC). Wound healing efficiency was studied using cell viability, proliferation, and scratch assays in diabetic human dermal fibroblast (HDF-D) cells. Results: The EtOAc fraction showed moderate activity against all bacterial strains tested, and the maximum inhibition zone was observed against Streptococcus pyogenes (30 mm in diameter). The fraction showed higher sensitivity to Gram-positive strains than Gram-negative strains. In the quantitative analysis of antioxidant content, the EtOAc fraction was found to have a TPC of 65.81 ± 0.01. The DPPH scavenging activity and the hydrogen peroxide assay were correlated with the TPC value, with IC50values of 18.21 ± 0.06 and 59.22 ± 0.04, respectively. The wound healing experiment revealed a significant enhancement of cell proliferation and migration of HDF-D cells. GC-MS analysis confirmed the presence of 17 bioactive constituents that may be the principal factors in the significant antibacterial, antioxidant, and wound healing activity. Conclusion: The EtOAc fraction of MO leaves possesses remarkable wound healing properties, which can be attributed to the antibacterial and antioxidant activities of the fraction.
Abbreviations used: MO: Moringa oleifera; EtOAc: Ethyl acetate; GC-MS: Gas Chromatography-Mass Spectrometry; HDF-D: Diabetic Human Dermal Fibroblast cells.
Keywords: Diabetic wound healing, gas chromatography-mass spectrometry, migration rate, phenolic content, scratch assay, skin pathogen
|How to cite this article:|
Gothai S, Muniandy K, Zarin MA, Sean TW, Kumar S S, Munusamy MA, Fakurazi S, Arulselvan P. Chemical composition of Moringa oleifera ethyl acetate fraction and its biological activity in diabetic human dermal fibroblasts. Phcog Mag 2017;13, Suppl S3:462-9
|How to cite this URL:|
Gothai S, Muniandy K, Zarin MA, Sean TW, Kumar S S, Munusamy MA, Fakurazi S, Arulselvan P. Chemical composition of Moringa oleifera ethyl acetate fraction and its biological activity in diabetic human dermal fibroblasts. Phcog Mag [serial online] 2017 [cited 2021 Oct 26];13, Suppl S3:462-9. Available from: http://www.phcog.com/text.asp?2017/13/51/462/216304
- Moringa oleifera (MO) leaf ethyl acetate (EtOAc) fraction possesses antibacterial activities toward Gram-positive bacteria such as Streptococcus pyogenes, Streptococcus faecalis, Bacillus subtilis, Bacillus cereus and Staphylococcus aureus, and Gram-negative bacteria such as Proteus mirabilis and Salmonella typhimurium
- MO leaf EtOAc fraction contained the phenolic content of 65.81 ± 0.01 and flavonoid content of 37.1 ± 0.03, respectively. In addition, the fraction contained 17 bioactive constituents associated with the antibacterial, antioxidant, and wound healing properties that were identified using gas chromatography-mass spectrometry analysis
- MO leaf EtOAc fraction supports wound closure rate about 80% for treatments when compared with control group.
| Introduction|| |
The rising epidemic of diabetes is resulting in a calamitous physical, emotional, and financial toll on our country, Malaysia as well as worldwide. The World Health Organization anticipates that by 2030, more than 347 million people worldwide will suffer from diabetes; this is 10 times greater than the number of people affected by HIV/AIDS. Patients who develop diabetes will be subject to debilitating complications and higher health-care costs. More than 298 million cases are expected in developing countries, where most patients will not have access to adequate healthcare. Diabetic foot ulcers (DFUs), a leading cause of amputations, affect 15% of people with diabetes. Due to the lack of healing in diabetic patients, the ulcers represent a substantial morbid event. DFUs increase the risk of infections and other associated complications. Five-year mortality rates after new-onset diabetic ulceration have been reported to be between 43% and 55%, and rise to 74% for patients receiving lower-extremity amputation. The magnitude of the challenge faced by DFU patients is reflected in the high cost of treatment.
DFUs often fail to heal because of a persistently high concentration of pro-inflammatory cytokines in the wound site. Pro-inflammatory cytokines are strongly upregulated in hyperglycemic conditions. A high glucose concentration substantially disturbs cell-dependent responses and induces high concentrations of proteases, which degrades multiple growth factors, receptors, and matrix proteins that are essential for wound healing. In addition, the diabetic wound does not follow the orderly cascade of events that characterize normal wound healing. Instead, the inflammatory reaction in diabetic wounds is prolonged. Thus, the end results are ongoing tissue destruction rather than repair. In addition, a longstanding open wound generates vulnerability to opportunistic infections, which aggravate the healing process. Open wounds are susceptible to infection, especially by bacteria, and also offer an entry point for systemic infections.
One of the most important considerations in caring for patients with DFU is the avoidance of serious complications by expeditious and complete wound healing. Regardless of the multitude of wound healing drugs and technologies that have been reported in recent years, the management of chronic diabetic ulcers remains a challenge among biomedical researchers. The emerging scientific perspective of wound physiology has introduced natural therapeutic approaches targeting specific wound healing abnormalities. Antibacterial and healing compounds present in a traditional remedy can support this approach and may be valuable in the treatment of wounds. Virtually all diabetes involves free radicals and although the free radicals are secondary to the disease; in some cases, the free radicals are causal and lead to oxidative stress. Cellular antioxidants augment wound healing by a reduction of the damage caused by oxygen radicals. The antioxidant complement in diabetic patients is massively depleted by free radicals. Thus, a delicate balance between free radicals and antioxidants is essential to ensure healthy wound healing in diabetic patients. Studies have confirmed that medicinal plants with high antioxidant polyphenol content are cost-effective, efficacious wound healing agents in the management of diabetes, with few side effects.
The growing body of evidence of the effectiveness of Moringa oleifera (MO) as a wound healing agent has led to a significant increase in its uses. Different parts of this plant act as antihyperthyroidism agents, natural coagulants, and possess antitumor, antiinflammatory, antiulcer, antispasmodic, antihypertensive, cholesterol-lowering,, antidiabetic, hepatoprotective, antibacterial, and antifungal activities. This versatility encouraged us to explore the effect of the ethyl acetate (EtOAc) fraction of MO leaves on the different parameters of wound healing, including antibacterial activity, antioxidant activity, cell proliferation, and migration rate in an in vitro wound healing model. In addition, we identified the constituent chemical compounds of the fraction by gas chromatography-mass spectrometry (GC-MS) analysis.
| Materials and Methods|| |
Plant collection and extract preparation
Fresh and mature MO leaves were acquired from Garden No. 2 at Universiti Putra Malaysia (UPM), Malaysia, with the voucher specimen SK 1561/08 and stored in the IBS Herbarium unit. The leaves were further extracted with 90% ethanol and then filtered through Whatman filter paper. The filtrates were evaporated on a rotary evaporator, and the extracts were concentrated under reduced pressure and lyophilized to obtain a powder (Virtis Bench Top K, United States). The ethanol extract was extracted successively with different organic solvents such as hexane, chloroform, EtOAc, and butanol to obtain the hexane, EtOAc, chloroform, and butanol fractions, respectively, in addition to the residual methanol fraction. All crude extracts were filtered separately through Whatman No. 41 filter paper to remove particles and were evaporated to dryness in a rotary evaporator. The residue left in the separating funnel was reextracted twice following the same procedure and then filtered. The combined extracts were concentrated and dried in a rotary evaporator under reduced pressure. Only the MO EtOAc fraction was used in further experiments, and this was stored at − 20°C.
Agar disc diffusion method
The antibacterial effects of the MO leaf EtOAc fraction were evaluated using agar disc diffusion method. A total of 105 CFU/mL of overnight bacteria culture was spread on agar. Numbers of sterilized discs were dipped into the solvents (negative controls) and fraction solutions (100 mg/mL) and placed on the plates. After incubation at 37°C for 24 h, the antibacterial activity was assessed by the measurement of the diameter of the inhibition zone formed around the disc. A comparison test of the antibiotic control, streptomycin, was made using commercial discs. The antibacterial experiments were conducted in triplicate and inhibition zone diameters were recorded from each independent experiment.
Total phenolic content
The total phenolic content (TPC) in the MO leaf EtOAc fraction was estimated using Folin-Ciocalteu reagent (Singleton et al., 1999). In a series of test tubes, 0.5 mL of the fraction in methanol was mixed with 2.5 mL of 10% Folin-Ciocalteau reagent and 2.5 mL of 7.5% sodium carbonate. After shaking, the mixture was left to stand for 2 h and then the absorbance was measured at 765 nm. A standard curve was prepared using gallic acid. From the standard curve, the TPC was calculated and expressed as gallic acid equivalent in mg/g of fraction.
Total flavonoid content
The total flavonoid content was measured by a colorimetric assay. 1 mL of extract was added to 1 mL of distilled water, then 0.15 mL of 2% aluminum chloride solution was added, and the sample was thoroughly mixed. The absorbance at 415 nm was measured relative to a blank sample. Rutin was used as the standard for the calibration curve. The total flavonoid content of the extract was expressed as rutin equivalent in mg/g of sample.
1-diphenyl-2-picryl hydrazyl free radical scavenging assay
The free radical scavenging activity of the fractions was measured in vitro by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay. DPPH solution (0.1 mM in methanol) was prepared, and 150 μL of this solution was mixed with 150 μL of fraction or ascorbic acid (standard) in methanol at various concentrations. The reaction mixture was vortexed thoroughly, left in the dark at room temperature for 30 min, and the absorbance was measured at 517 nm. The percentage inhibition of scavenging was determined by the following equation:
Where A 0 is the absorbance of DPPH radicals and methanol, and A 1 is the absorbance of DPPH radicals and sample extract or standard.
The IC50 values of the fraction and standard were determined. All analyses were performed in triplicate, and the average was reported.
Hydrogen peroxide scavenging assay
The hydrogen peroxide radical scavenging activity of the MO leaf EtOAc fraction was estimated using the method of  with a rational modification. Hydrogen peroxide solution (4 mM) was prepared in a standard phosphate buffer (pH 7.4). Briefly, 174 μL of various concentrations of fraction solutions and the standard (ascorbic acid) were mixed with 26 μL H2O2 solution (2 mM) and incubated for 10 min. After incubation, the absorbance was measured at 230 nm relative to a blank solution containing phosphate buffer without hydrogen peroxide. The percentage inhibition of hydrogen peroxide radicals scavenged by the fraction was calculated using this formula:
Where A 0 is the absorbance of the control, and A 1 is the absorbance in the presence of the MO leaf EtOAc fraction.
In vitro diabetic wound healing study
Cell culture and cell viability assay
Diabetic human dermal fibroblast cells (HDF-D, ZenBio Inc.) were cultured in Dulbecco's Modified Eagle Medium containing 5% fetal bovine serum, 100 μg/mL streptomycin, and 100 U/mL penicillin at 37°C in an atmosphere of 5% CO2. The effect of the EtOAc fraction on cell cytotoxicity was investigated by 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay in HDF-D. Briefly, cultured cells were seeded into a 96-well plate at a density of 1 × 105 and left overnight. Subsequently, the cells were treated with serial dilutions of MO leaf EtOAc fraction of 15–500 μg/mL. After a 24-h incubation period, 10 μL MTT reagent (5 mg/mL) was then added to each of the wells and incubated for 4 h. The resulting formazan crystals were dissolved by the addition of 100 μL dimethyl sulfoxide to each well, well mixed, and left to stand in the dark at room temperature for 30 min. Absorbance was recorded at 570 nm using an ELISA microplate reader, and the cell viability was calculated according by comparing with the control group without treatment.
Cell proliferation assay
Briefly, HDF-D cells were seeded on 96-well plates at a density of 1 × 105 cell/well and incubated at 37°C overnight. The cells were treated with various concentrations (15–500 μg/mL) of the MO leaf EtOAc fraction as described in the cell viability section and incubated for 24 h. The plates were then incubated with 10 μL of Cell Counting Kit-8 (CCK-8) solution for 4 h. The absorbance of samples was measured by microplate reader at 450 nm with a reference at 630 nm. To determine the cell proliferation rate, the graph of absorbance against the number of cells was plotted according to the manufacturer's instructions. All the experiments were performed in triplicate.
The scratch assay was conducted according to a previously established method in our research group. Briefly, HDF-D cells at a cell density of 2 × 105 cells/well were seeded into a 24-well plate and incubated at 37°C in an atmosphere of 5% CO2. After incubation, cells were scraped with a P200 pipette tip, and the media was removed from each well. MO leaf EtOAc fractions (12.5, 25, and 50 μg/mL), and the positive control drug, allantoin, were added to each well and photographed by phase contrast microscopy at ×4 magnification at 0 h. After an incubation period of 24 h, the second set of images was recorded. To determine the percentage of cell migration rate, the images were analyzed by ImageJ software (NIH, Bethesda, MD, USA).
Gas chromatography-mass spectrometry analysis
The GC-MS analysis of the MO leaf EtOAc fraction was performed using an Agilent 6890N series II GC interfaced with an Agilent 5973 series quadrupole mass spectrometer (Palo Alto, CA, USA) and equipped with an Agilent 76,73A autosampler. Helium gas (99.999%) was used as the carrier gas with a constant flow rate of approximately 1 mL/min. Mass transfer line and injector temperatures were set at 220°C and 290°C, respectively. The oven temperature was set to increase from 50°C to 150°C at 3°C/min, held isothermally for 10 min, and finally raised to 250°C at 10°C/min.
The MO leaf EtOAc fractions were diluted to 10 mg/mL in methanol. The diluted samples were injected in the split mode with a split ratio of 120:1. The delay time was 2 min, and the total running time was 120 min. The relative amounts of the chemical ingredients in the MO leaf EtOAc fractions were expressed as a percentage by peak area normalization. The relative percentage of each component was determined by comparing its average peak area to the total area. The software used to handle mass spectra and chromatograms was a GC-MS solution (Version 2.53).
Data are presented as the mean ± standard deviation. The statistical significance between groups was analyzed by one-way ANOVA using SPSS software version 21.0 (SPSS, Chicago, Illinois, USA). The results obtained at the end of experiment were compared with those of the control and diabetic groups using a Student's t-test. Values of *P < 0.05, **P < 0.01, and ***P < 0.001 were considered statistically significant.
| Results|| |
Antibacterial activity of Moringa oleifera ethyl acetate fraction
The antibacterial activity of the MO leaf EtOAc fraction was tested against 16 pathogenic bacterial strains: six Gram-positive (Staphylococcus aureus, Streptococcus faecalis, Streptococcus pyogenes, Bacillus subtilis, Bacillus cereus, and Bacillus thuringiensis) and 10 Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Salmonella typhimurium, Pseudomonas solanacearum, Enterobacter aerogenes, Acinetobacter anitratus, Klebsiella pneumonia, Serratia marcescens, Staphylococcus epidermidis, and Salmonella choleraesuis). These bacterial strains were selected on the basis of their skin infection-causing behavior in diabetic patients. The results of the antibacterial activities are presented in [Table 1]. The MO leaf EtOAc fraction produced inhibition zone diameters ranging from 7 to 31 mm, and the most noteworthy results were exhibited by Gram-positive bacteria. The diameters were calculated including the 6-mm filter paper disc. Streptomycin was used as the standard antibiotic and showed significant antibacterial activity against all the test organisms.
|Table 1: Antibacterial activity of Moringa oleifera leaf ethyl acetate fractions by agar disc diffusion method|
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Antioxidant activity of Moringa oleifera ethyl acetate fraction
Total phenolic content and total flavonoid content
[Table 2] shows the total phenolic and total flavonoid contents of the MO leaf EtOAc fraction. The TPC was calculated from the calibration curve (y = 0.0015x + 0.0475) with a regression value of 0.9931, and the MO leaf EtOAc fraction had a TPC value of 65.81 ± 0.01 mg/g. This value indicated that 1 mg of plant extract contained an amount of phenol equivalent to 65.81 mg of pure gallic acid. TPC in the EtOAc fraction is predicted to be higher than other solvent fractions. In short, the results suggest the MO leaf EtOAc fraction is a rich source of polyphenolic compounds. The total flavonoid content of the MO leaf EtOAc fraction is presented in [Table 2]. The total flavonoid content was calculated from the calibration curve (y = 0.001x + 0.0635) with a regression value 0.9964. From the rutin standard curve of various concentrations, the MO leaf EtOAc fraction had a total flavonoid content of 37.1 ± 0.03 mg/g.
|Table 2: Total phenolic and total flavonoid content in the Moringa oleifera leaf ethyl acetate fraction|
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1-diphenyl-2-picryl hydrazyl free radical scavenging activity
The scavenging effects of the MO leaf EtOAc fraction on the DPPH radical are illustrated in [Figure 1] and show that the fraction significantly reduced DPPH radicals. The scavenging activity increased in a concentration-dependent manner due to the scavenging capacity of the fraction and was comparable to ascorbic acid. The IC50 value signifies the concentration required to scavenge 50% of the initial DPPH radicals. The IC50 value of the MO leaf EtOAc fraction was 18.21 ± 0.06 μg/mL; in comparison, the standard (ascorbic acid) had an IC50 of 13.78 ± 0.02 μg/mL.
|Figure 1: 1-diphenyl-2-picryl hydrazyl scavenging activity of the ethyl acetate fraction of Moringa oleifera leaves at various concentrations. Values are mean ± standard deviation (n = 3). The 1-diphenyl-2-picryl hydrazyl scavenging activity of ethyl acetate fraction increased in a concentration-dependent manner comparable to ascorbic acid|
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Hydrogen peroxide scavenging activity
[Figure 2] shows the scavenging activity of the MO leaf EtOAc fraction on hydrogen peroxide. The fraction scavenged hydrogen peroxide in a concentration-dependent manner. Evaluation of the IC50 shown in [Figure 2] demonstrated that the fraction exhibited moderate antioxidant activity with an IC50 value of 59.22 ± 0.04 μg/mL. This result was lower than the reference standard, ascorbic acid (IC50: 47.27 ± 0.09 μg/mL).
|Figure 2: Hydrogen peroxide scavenging activity of Moringa oleifera leaf ethyl acetate fraction at various concentrations. Values are mean ± SD (n = 3). The H2O2 radical inhibition activity of ethyl acetate fraction increased in a concentration-dependent manner comparable to ascorbic acid|
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Effect of Moringa oleifera ethyl acetate fraction on cell viability
The cytotoxic effect of isolated MO leaf EtOAc fraction was evaluated by MTT assay on HDF-D cells exposed to various concentrations (15.62, 31.25, 62.5, 125, 250, and 500 μg/mL) of fraction. Cell survival analyses [Figure 3] showed concentration-dependent inhibition of the fraction on HDF-D cell growth. Higher concentrations (125, 250, and 500 μg/mL) of the fraction increased cytotoxicity by about 40% in HDF-D cells; hence, we selected a nontoxic concentration of the fraction to investigate its wound healing properties.
|Figure 3: The cytotoxicity of Moringa oleifera leaf ethyl acetate fraction treatment in diabetic human dermal fibroblast cells was assessed. Cells were treated with various concentrations of isolated fractions (15.62, 31.25, 62.5, 125, 250, and 500 μg/mL) for 24 h. After the incubation period, the viability of fraction-treated cells was evaluated by 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide assay. Values are presented as the mean percentage ± standard deviation of three replicates. ***P < 0.001 versus control group|
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Effect of Moringa oleifera ethyl acetate fraction on cell proliferation
The cell proliferative properties of MO leaf EtOAc fraction were evaluated in HDF-D cells using a CCK-8 assay kit according to the manufacturer's instructions. Cells were treated with a serial dilution of the EtOAc fraction for 24 h as described in the cell viability section. As shown in [Figure 4], treatment with this fraction caused an irregular fluctuation in the percentage of cells, which indicated that the fraction did not exert a concentration-dependent effect on the growth of HDF-D cells although lower concentrations of the fraction slightly increased cell proliferation.
|Figure 4: The proliferative effect of the Moringa oleifera leaf ethyl acetate fraction on diabetic human dermal fibroblast cells. Cells were seeded in a 96-well plate, different concentrations of Moringa oleifera ethyl acetate fraction were added, and the cells were left to stand for 24 h. The proliferative effect was measured by Cell Counting Kit-8 assay kit and calculated by a comparison of the values from the ethyl acetate fraction treatment group with the control group. Data are expressed as mean ± standard deviation from three individual experiments. *P < 0.05, **P < 0.01, ***P < 0.001 versus control group|
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Effect of Moringa oleifera on wound healing activity
In vitro migration assays were performed to investigate the wound healing potential of the MO leaf EtOAc fraction in HDF-D cells [Figure 5] and [Figure 6]. Different concentrations of the MO EtOAc leaves fraction (12.5, 25, and 50 μg/mL) were applied for 24 h after wound scratching. The wound coverage was significantly increased for HDF-D cells at all concentrations compared with the control group. Although the control group cells slightly closed the scratched area, MO leaf EtOAc fraction-treated HDF-D cells were found to migrate faster following a 24-h incubation period. Rapid cell migration and wound closure rate of the fraction-treated HDF-D cells was observed, and these effects were comparable with the positive control drug allantoin, especially at a treatment concentration of 25 μg/mL [Figure 6]. In comparison with the control group, wound closure rates were enhanced by 82% and 87% for treatment.
|Figure 5: The percentage of cell migration rate of Moringa oleifera leaf ethyl acetate fraction treated wound in diabetic human dermal fibroblast. Cells were seeded into 6-well plates with the addition of different concentrations of Moringa oleifera ethyl acetate leaves fraction for 24 h. The migration rate of Moringa oleifera leaf ethyl acetate fraction-treated wounds in diabetic human dermal fibroblast cells was evaluated at 0 and 24 h after treatment and calculated by Image-J software. Results are expressed as mean ± standard deviation from each individual experiment. *P < 0.05, **P < 0.01***P < 0.001 versus control group|
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|Figure 6: The migration rate of the fraction on diabetic human dermal fibroblast cells. The various concentrations (12.5, 25, and 50 μg/mL) of Moringa oleifera leaf ethyl acetate fraction were treated on a wound created in diabetic human dermal fibroblast observed by scratch assay. Human dermal fibroblast-diabetic cells were scratched with p200 pipette tips, and photos were captured at 0 h. After the treatment with various concentrations of Moringa oleifera leaf ethyl acetate fraction, the cells were photographed after 24 h incubation (a) Control, (b) Moringa oleifera leaf ethyl acetate fraction 12.5 μg/mL, (c) Moringa oleifera leaf ethyl acetate fraction 25 μg/mL, (d) Moringa oleifera leaf ethyl acetate fraction 50 μg/mL, (e) allantoin 50 μg/mL|
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Gas chromatography-mass spectrometry analysis
Interpretation of the GC-MS spectra of the MO leaf EtOAc fraction was conducted using the National Institute Standard and Technique (NIST) database. The spectrum of the unidentified components was compared with the spectra of known components stored in the NIST library. Qualitative analyses of the fraction using GC-MS showed the presence of 17 prominent peaks in the chromatogram out of a total of 30 [Figure 7]. The peak area concentration (%), retention time, and peak identities of the compounds are presented in [Table 3].
|Figure 7: Gas chromatogram obtained for ethyl acetate fraction of Moringa oleifera leaf extract|
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|Table 3: Total ion chromatogram of Moringa oleifera leaf ethyl acetate fraction with retention time, peak area, and reported biological activities|
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| Discussion|| |
MO is known as a valuable food source because of its high nutritional content and physiological properties. The principal constituents of MO leaves are water, protein, sugar, mineral salts, and fatty acids., Several of these sugar substances are pharmacologically active, including L-arabinose, D-mannose, D-galactose, L-rhamnose, and D-xylose and have been shown to enhance wound healing in multiple extensive studies. Furthermore, many researchers have demonstrated that natural sugars, such as D-mannose and D-glucose, have wide variety of antimicrobial functionalities., In addition, MO leaves contain many fatty acids including lauric acid, myristic acid, palmitic acid, arachidonic acid, and oleic acid , that have a similar molecular structure to 10-HDA. 10-HDA is a bioactive compound found in royal honey that has been shown to enhance wound healing in various extensive studies. This type of fatty acids triggers fibroblasts to induce various growth factors in the wound, in particular transforming growth factor-β1 (TGF-β1) and vascular endothelial growth factor (VEGF).
In vitro antibacterial analysis of MO leaf EtOAc fraction showed a potential inhibition of Gram-positive bacteria but only moderate activity against Gram-negative bacteria. The difference in antibacterial efficiency may be attributed to the different bacterial structures: the cell wall in Gram-positive bacteria consists of a single layer while the Gram-negative cell wall is a multilayered structure confined by an outer cell membrane. Our findings indicated that the MO leaf EtOAc fraction may be a novel candidate for dermal wound healing because of the effectiveness of its antibacterial properties against various pathogens, especially skin infection-causing pathogens.
Phenols are major phytoconstituents in plants; flavonoids are primarily derivatives of phenolic compounds. Phenolics act mainly as free radical scavengers, conferring oxidative stress tolerance on plants. In the present study, phenolic levels in MO leaf EtOAc MO fraction were found to be relatively high and the estimation of antioxidant activity of showed a positive correlation between antioxidant activity and phenolic content. However, the flavonoid content the fraction was much lower than the phenol content. A similar trend of antioxidant content in MO leaves was reported by Iqbal and Bhanger. Antioxidants present in plants enhance the healing of wounds by quenching the free radicals and the prevention of the cellular damage caused by free radicals. The redox properties of antioxidants could delay or prevent the onset of degenerative diseases. This property allows them to act as hydrogen donors or reducing agents, which improves regeneration and organization of the new tissue in wound healing. Thus, the enhanced wound healing may be due to the free radical scavenging action of the fraction.
According to Guo and Dipietro, healing impairment in diabetic ulcers has a number of physiological causes including diminished fibroblast proliferation and angiogenesis. The wound healing effect on HDF-D after treatment with the fraction supports the wound healing activity by promotion of the proliferation of fibroblast cells. As the crucial migration step of the wound healing process is impaired in diabetes, the fraction demonstrated a potent promotion of migration as determined by the increased response in migration scratch assay. Proliferation and migration are the manifestations for the development of new blood vessels from the preexisting vascular bed in angiogenesis.
The growth factors TGF-β and VEGF are involved in fibroblast migration and angiogenesis, respectively. The increased migration rate observed in HDF-D cells may be the influence of growth factors which are involved in the wound healing process. The wound healing properties may be attributed to the phytocompounds presents in the EtOAc fraction. These statements were justified by the GC-MS analysis of the fraction which identified various medicinal compounds with wound healing effects including antioxidant, antibacterial, anti-inflammatory, and antidiabetic activity. Therefore, the MO leaf EtOAc fraction is a therapeutically beneficial agent with vital bioactive compounds that can improve diabetic wound healing by the stimulation of fibroblast growth, promotion of angiogenesis, and acceleration of healing rate with antibacterial properties.
| Conclusion|| |
Our results demonstrated that the MO leaf EtOAc fraction has significant wound healing activity. These conclusions validate the use of this plant in folkloric medicine for the treatment of wounds and provide valuable scientific evidence that the EtOAc fraction was a promising complementary supplement for diabetic patients with wound healing defects. Further in vivo experiments on the detailed molecular mechanisms responsible for the enhancement the wound healing treatments in diabetes need to be conducted.
This research study was supported by the Research Management Centre, UPM (Project No: GP-1/2014/9443700), Malaysia. This work was also supported by the King Saud University, Deanship of Scientific Research, and College, of Sciences Research Center, King Saud University.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
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[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7]
[Table 1], [Table 2], [Table 3]