| ORIGINAL ARTICLE |
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| Year : 2017 | Volume
: 13
| Issue : 51 | Page : 446-453 |
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Optimization of surfactant-mediated, ultrasonic-assisted extraction of antioxidant polyphenols from rattan tea (Ampelopsis grossedentata) using response surface methodology
Feng Li1, Aun Raza2, Yan-Wei Wang2, Xiu-Quan Xu2, Guan-Hua Chen3
1 Department of Food Science, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212001, Jiangsu Province; Department of Cardiothoracic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, P.R. China
2 Department of Pharmacognosy, School of Pharmacy, Jiangsu University, Zhenjiang 212013, P.R. China
3 Department of Food Science, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212001, Jiangsu Province, P. R. China
Correspondence Address:
Xiu-Quan Xu
301, Xuefu Road, Zhenjiang 212013, Jiangsu Province
P.R. China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/pm.pm_159_16
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Background: Rattan tea is a medicinal plant that has been used for many years for the treatment of inflammation, fatty liver, tumor, diabetes, and hyperlipidemia. Objective: A green and novel approach based on surfactant-mediated, ultrasonic-assisted extraction (SM-UAE) was developed for the extraction of antioxidant polyphenols from Rattan tea. A nonionic surfactant Tween-80 was selected as extraction solvent. The antioxidant activity was measured by total phenolic content (TPC) and ferric-reducing/antioxidant capacity (FRAC) assay. Materials and Methods: Optimization of extraction parameters including concentration of solvent, ultrasonic time, and temperature were investigated by response surface methodology. The antioxidant activity was measured by TPC and FRAC assay. Results: The optimal extraction conditions were determined as 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54°C, and ultrasonic time of 28.8 min. Under these conditions, the highest TPC value of 360.4 mg gallic acid equivalent per gram of dry weight material (GAE/g DW) was recorded. Moreover, 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54.5°C, and ultrasonic time of 28.4 min were determined for the highest FRAC value of 478.2 μmol of Fe2+/g of weight material (μmol Fe2+/g DW). Compared with other methods, the TPC and FRAC values of 313.5 mg GAE/g DW and 389.6 μmol Fe2+/g DW were obtained by heat reflux extraction using ethanol as solvent, respectively, and 343.2 mg GAE/g DW and 450.1 μmol Fe2+/g DW were obtained by UAE using ethanol as solvent, respectively. Conclusion: The application of SM-UAE markedly decreased extraction time or extraction cost and improved the extraction efficiency, compared with the other methods.
Abbreviations used: SM-UAE: Surfactant-mediated ultrasonic-assisted extraction; TPC: total phenolic content; FRAC: Ferric reducing antioxidant capacity; RSM: Response surface methodology; BBD: Box-Behnken design. |
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