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Year : 2017  |  Volume : 13  |  Issue : 49  |  Page : 26-32

Influence of capsaicin on inflammatory cytokines induced by lipopolysaccharide in myoblast cells under in vitro environment

1 Department of Animal Science and Biotechnology, Chonbuk National University, Jeonju, Republic of Korea
2 Department of Biology, Faculty of Science, Albaha University, Albaha, Republic of Korea
3 Department of Pharmacognosy, Pharmacy College, King Saud University, Riyadh, Saudi Arabia
4 Petrochemical Research Chair, Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia

Correspondence Address:
Dr. Inho Hwang
Department of Biology, Faculty of Science, Albaha University, Albaha
Republic of Korea
Dr. Touseef Amna
Department of Animal Science and Biotechnology, Chonbuk National University, Jeonju
Republic of Korea
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1296.203984

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Background: Cellular damage initiated by reactive oxygen species (ROS) is the main cause of numerous severe diseases and therefore for this reason, the natural antioxidants have note worthy significance in human health. Capsaicin possesses noteworthy analgesic and anti-inflammatory properties. It also possesses healing effects for treatment of arthritis, diabetic neuropathy, gastric lesions, and cardiac excitability that is why it is incorporated in creams and gels. Objective: The present study was carried out to estimate the in vitro antioxidant and ROS scavenging activities of capsaicin against muscle precursor cells. Till date,no investigation has been carried out to study the effect of capsaicin on myoblasts.Materials and methods: Herein, the cytotoxicity was induced by endotoxin lipopolysaccharide (LPS) to analyze the effect of capsaicin on LPS induced inflammation and apoptosis on muscle cells. To find out the toxicity of endotoxin, myoblasts were exposed to different concentrations of LPS, viability and morphology was checkedby the means of CCK-8 test and microscopy, respectively. Apoptotic cell death was examined by fluorescence staining. Additionally, LPS-induced apoptosis was determined by mRNAexpression of calpain, caspase-3 and tumor necrosisfactor alpha (TNF-α), and were quantified by qRT-PCR. Results: The outcome of the presentstudy demonstrated that LPS stimulation generatestoxicity in dose-dependent manner. Pre-treatmentof myoblasts with capsaicin can considerably alleviate LPS-induced inflammation. Conclusion: In conclusion, this study indicates that dietetic supplementation of capsicum may help to alleviate/reduce the inflammatory effects and is therefore potent source of natural antioxidant agent which can be utilized to control muscle related diseases, such as myotube atrophy. Abbreviation used: AMP: Adenosine monophosphate, AO/EB: Acridine orange / Ethidium bromide, ATL: T-cell leukemi, CAP: Capsaicin, CCK-8: Cell counting Kit-8, CLSM: Laser Scanning Microscopy, DCF-DA: 2', 7'-dichlorofluorescein diacetate, DMEM: Dulbecco's modified Eagle's medium, DPPH: α, α-diphenyl-β-picrylhydrazyl, FBS: Fetal bovine serum, KA: Kainic acid, LPS: Lipopolysaccharide, MDA: Malondialdehyde, NF-kB: Nuclear factor kgene binding, PBS: Phosphate buffer saline, pNA: p-nitroanilide, RNW: RNase free water, ROS: Reactive oxygen species, TNF-α: Tumor necrosis factor alpha, TRPV1: Transient receptor potential vanilloid 1

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