| ORIGINAL ARTICLE |
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| Year : 2016 | Volume
: 12
| Issue : 47 | Page : 482-487 |
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Elucidation of molecular mechanism(s) of cognition enhancing activity of Bacomind® : A standardized extract of bacopa monnieri
Shekhar Dethe, M Deepak, Amit Agarwal
Natural Remedies R and D Centre No. 5B, Veerasandra, Bangalore, India
Correspondence Address:
Shekhar Dethe
Natural Remedies R and D Centre No. 5B, Veerasandra Industrial Area, 19th km, Hosur Road, Electronic City, Bangalore
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0973-1296.191464
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Background: Bacopa monnieri (L.) Wettst., commonly known as Brahmi, is renowned in Indian traditional system for its potent memory enhancing activity, which has been validated by various scientific studies. Objective: The objective of this study was to understand the molecular mechanism of memory enhancing activity of BacoMind® (BM), a standardized extract of B. monnieri. Materials and Methods: BM was screened in vitro in a panel of cell-free and receptor-transfected cell assays. The purified enzymes/membrane homogenates/cells were incubated with substrate/standard ligand in the absence or presence of the test compound. The IC 50 values and EC 50 values were determined by nonlinear regression analysis of the concentration-response curves generated with mean replicate values using Hill equation curve fitting. Results: BM was found to inhibit three enzymes; Catechol-O-methyl transferase (COMT), Prolyl endopeptidase (PEP), and Poly (ADP-ribose) polymerase (PARP). It also had an antagonistic effect on serotonin 6 and 2A (5-HT 6 and 5-HT 2A ) receptors , known to influence the different neurological pathways, associated with memory and learning disorders, age-associated memory impairment. Conclusion: BM was found to inhibit three enzymes namely, Catechol-O-methyl transferase (COMT), Prolyl endopeptidase (PEP), and Poly (ADP-ribose) polymerase (PARP). It also exhibited an antagonistic effect on 5-HT 6 and 5-HT 2A receptors.
Abbreviations used: HTRF: Homogenous time resolved fluorescence, cAMP: Cyclic adenosine monophosphate, CHO : Chinese hamster ovary, RFU : Relative fluorescence unit, pNP: Para nitro phenol, AMC: 7-amino-4-methylcoumarin, ELISA: Enzyme linked immunosorbent assay, Z-Pro-Pro-CHO: Z-prolyl-prolinal, HEK: Human embryonic kidney, TE: Trolox equivalent. |
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