Liquid chromatography-diode array detector-electrospray mass spectrometry and principal components analyses of raw and processed moutan cortex
Xian-Mei Deng1, Jiang-Yong Yu2, Meng-Jin Ding1, Ming Zhao3, Xing-Yang Xue4, Chun-Tao Che3, Shu-Mei Wang1, Bin Zhao5, Jiang Meng1
1 Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China
2 Devision of Traditional Chinese Medicines Standard, Chinese Pharmacopoeia Commission, Beijing 100061, China
3 Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA
4 Guangzhou Medical University Cancer Hospital and Institute, Guangzhou, 510000, China
5 Zhongshan Torch Polytechnic, Zhongshan, 528436, China
|Date of Web Publication||10-Feb-2016|
Zhongshan Torch Polytechnic, Zhongshan 528436
Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Raw Moutan Cortex (RMC), derived from the root bark of Paeonia suffruticosa, and Processed Moutan Cortex (PMC) is obtained from RMC by undergoing a stir-frying process. Both of them are indicated for different pharmacodynamic action in traditional Chinese medicine, and they have been used in China and other Asian countries for thousands of years. Objective: To establish a method to study the RMC and PMC, revealing their different chemical composition by fingerprint, qualitative, and quantitative ways. Materials and Methods: High-performance liquid chromatography coupled with diode array detector and electrospray mass spectrometry (HPLC-DAD-ESIMS) were used for the analysis. Therefore, the analytes were separated on an Ultimate TM XB-C18 analytical column (250 mm × 4.6 mm, 5.0 μm) with a gradient elution program by a mobile phase consisting of acetonitrile and 0.1% (v/v) formic acid water solution. The flow rate, injection volume, detection wavelength, and column temperature were set at 1.0 mL/min, 10 μL, 254 nm, and 30°C, respectively. Besides, principal components analysis and the test of significance were applied in data analysis. Results: The results clearly showed a significant difference among RMC and PMC, indicating the significant changes in their chemical compositions before and after the stir-frying process. Conclusion: The HPLC-DAD-ESIMS coupled with chemometrics analysis could be used for comprehensive quality evaluation of raw and processed Moutan Cortex.
Keywords: Fingerprints, high-performance liquid chromatography coupled with diode array detector and electrospray mass spectrometry, principal components analyses, quantification, raw and processed Moutan Cortex
|How to cite this article:|
Deng XM, Yu JY, Ding MJ, Zhao M, Xue XY, Che CT, Wang SM, Zhao B, Meng J. Liquid chromatography-diode array detector-electrospray mass spectrometry and principal components analyses of raw and processed moutan cortex. Phcog Mag 2016;12:50-6
|How to cite this URL:|
Deng XM, Yu JY, Ding MJ, Zhao M, Xue XY, Che CT, Wang SM, Zhao B, Meng J. Liquid chromatography-diode array detector-electrospray mass spectrometry and principal components analyses of raw and processed moutan cortex. Phcog Mag [serial online] 2016 [cited 2021 May 7];12:50-6. Available from: http://www.phcog.com/text.asp?2016/12/45/50/176046
Xian-Mei Deng†, Jiang-Yong Yu†
† These authors contributed equally to this work
- The experiment study the RMC and PMC by HPLC-DAD-ESIMS couple with chemometrics analysis. The results of their fingerprints, qualitative, and quantitative all clearly showed significant changes in their chemical compositions before and after stir-frying processed.
| Introduction|| |
Processing of herbal materials is a special and important pharmaceutical technique for the preparation of herbal drugs in traditional Chinese medicine (TCM).  While some herbs can be used as raw drugs after harvest from the field and undergoing simple postharvest treatments such as garbling, cleaning, and drying; others may require further specific processing procedures such as boiling, steaming, and frying or baking depending on the plant species. The former are often referred to as "raw herbal drugs," whereas the latter are "processed herbal drugs." As a result of the additional treatment processes, which involve prolonged heating, the therapeutic properties of the processed herbal drugs are often altered when compared to the raw drugs, showing reduced toxicity or enhanced efficacy. The processing of herbal materials can also improve the purity and flavor, or facilitate drug administration. ,,, Formal processing procedures for many TCM herbal drugs are documented in the Chinese Pharmacopoeia. 
Moutan Cortex, the root bark of Paeonia suffruticosa Andrews (Paeoniaceae), as a common TCM drug has been used in China and other Asian countries for thousands of years. Two kinds of crude drug preparations are available, the Raw Moutan Cortex (RMC) and the Processed Moutan Cortex (PMC). The RMC is prepared by collecting the root bark of P. suffruticosa, washed, cleaned, and sun-dried. The dried herbal materials are known as the "raw moutan bark." On the other hand, the PMC is produced after undergoing a further step, that is, stir-frying the dried raw materials until the bark surface turns into dark brown color. The stir-frying procedure involves continual stirring of the bark in a utensil heated to a high temperature. Such processed moutan material is also called "charcoaled moutan bark" in TCM. According to the TCM theory, the raw moutan bark serves to clear excessive heat, cool the blood, promote blood circulation, and remove blood stasis; it is indicated for diseases associated with the "heat syndromes" (typically manifesting in inflammation and related symptoms), stagnated blood conditions, and traumatic injuries. On the other hand, the charcoaled moutan bark not only possesses blood cooling property but more importantly, it is hemostatic. It is indicated for hemoptysis and other kinds of bleeding. , Pharmacological studies have demonstrated that the RMC possesses anti-inflammatory, analgesia, and blood activating properties while the PMC was able to restore hemostasis and promoting blood clotting. ,
Moutan Cortex has been extensively studied for its chemical composition. ,,,,,, Qualitative and quantitative analyses using various chemical markers, ,,,,,,, as well as quality control protocols, ,,,,,,, have been reported for this herbal drug. However, little information is available to demonstrate chemical changes, if any that may occur during the stir-frying process. In this study, PMC was prepared from RMC and their chromatographic fingerprints were established and compared; at the same time, qualitative and quantitative analyses of eight marker components were accomplished using the high-performance liquid chromatography coupled with diode array detector and electrospray mass spectrometry (HPLC-DAD-ESIMS) method. Fingerprinting similarity analysis, principal components analysis (PCA), and the test of significance were performed in an attempt to detect the chemical differences between RMC and PMC.
| Materials and Methods|| |
Chemicals and materials
Samples of Moutan Cortex were collected from different regions of China [Table 1] and authenticated by Dr. Shumei-Wang of the School of Chinese Medicine, Guangdong Pharmaceutical University. Voucher specimens were deposited in the University Herbarium. The RMC samples were prepared according to the procedures specified in the Chinese Pharmacopoeia. From each batch of the RMC samples, a portion was randomly taken to undergo the stir-frying process to produce the PMC in compliance with the standard procedures. 
|Table 1: Sources of RMC and PMC samples, and the results of similarity tests|
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Reference standards (purity >98%) of 5-hydroxymethylfurfural (5-HMF), gallic acid, catechins, oxypaeoniflorin, paeoniflorin, quercetin, paeonol, and benzoylpaeoniflorin were obtained from the National Institute for Food and Drug Control, Beijing, China. All the structures of the references are listed in [Figure 1]. HPLC-grade acetonitrile (Yu-Wang Chemical Factory, Shandong, China), formic acid (Beijing Reagent Co., Beijing, China), and analytical grade methanol (East giant Experimental Instrument Co., Guangzhou, China) were used. Water was purified by the Milli-Q water system (Millipore, Bedford, MA, USA).
|Figure 1: Chemical structures of the identified compounds (1) gallic acid, (2) 5-hydroxymethylfurfural, (7) catechin, (9) oxypaeoniflorin, (12) paeoniflorin, (19) quercetin, (20) benzoylpaeoniflorin, (22) paeonol|
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Apparatus and chromatographic condition
An Agilent 1100 series HPLC system equipped with a photodiode array detector (Agilent, Palo Alto, CA, USA.), a mass spectrometer with an ESI detector (Applied Biosystems, Foster City, CA, USA), and an Ultimate TM XB-C18 analytical column (250 mm × 4.6 mm, 5 μm, Alltech Associates, Deerfield, IL, USA) were used for all analysis. The mobile phase consisted of acetonitrile and 0.1% (v/v) formic acid in water, with the following gradient program: Acetonitrile was increased from 5% to 15% (first 30 min), then to 50% in the second 30 min, finally increased to 95% in the following 10 min. The flow rate, injection volume, detection wavelength, and column temperature were set at 1.0 mL/min, 10 μL, 254 nm, and 30°C, respectively. Data were processed by using Chemstation for LC3D (Hewlett Packard, Palo Alto, CA, USA). For mass detection, nitrogen was used as dry gas (7 L/min, 400°C). Both positive and negative ESI modes were employed. The values for sprayer voltage, orifice voltage, and focusing ring voltage were set at 5000 V, 101 V, and 380 V, respectively. The scan range was m/z 100-1000. All data acquired were processed by the MaccChrom 1.1 software (Applied Biosystems, Grand Island, NY, USA).
Each RMC and PMC sample was ground and passed through a 180-mesh sieve. The powder (0.5 g) was accurately weighed and extracted with 50% aqueous methanol (50 mL) for 30 min by sonication at room temperature. The suspension was centrifuged for 10 min at 20,000 rpm by an EBA-20S centrifuge (Hettich, Tuttlingen, Germany). The supernatant was transferred to a volumetric flask, made up to 100 mL with 50% methanol and shaken vigorously. An aliquot (10 μL) was applied to HPLC analysis after filtration through a 0.22-μm membrane filter. Each sample was analyzed in triplicate. The reference solution of a mixture of eight marker compounds was prepared in methanol and serially diluted to appropriate concentrations for the construction of calibration curves.
Similarity analysis was performed using a professional software, the Similarity Evaluation System for Chromatographic Fingerprint of TCM Version 2004A issued by the Chinese Pharmacopoeia Commission, which was recommended for use by the State Food and Drug Administration of China. The software employs correlative coefficients in the process of evaluating similarities of different chromatograms. 
PCA was performed on the contents of eight marker compounds by a software called Soft Independent Modeling of Class Analogy-P + 11.0. And independent-samples t-test was performed by the SPSS software (SPSS Statistics 16, SPSS Inc., USA).
Linearity of the HPLC method was evaluated by the calibration curves [Table 2]. A range of six concentrations of eight marker compounds was analyzed in triplicates. The limit of detection (LOD) and limit of quantitation (LOQ) for each analyte were determined as the signal-to-noise ratio of 3 and 10, respectively.
|Table 2: Calibration curves, linear ranges, LOD and LOQ of eight marker compounds|
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The precision of the method was determined by intraday and interday measurements. The standard solution was analyzed in six replicates on the same day to obtain the intraday precision results, and the same replicate samples were analyzed daily for three successive days to obtain the interday results. Stability of the sample solution was evaluated by the same RMC and PMC sample solutions at 0, 2, 4, 8, 12, and 24 h. Meanwhile, recovery tests were performed to determine the accuracy of the method, in which an accurate amount of each standard was added to 0.25 g of Moutan Cortex sample and triplicate experiments were carried out. The results were expressed as % relative standard deviation (RSD) of the measurements [Table 3].
| Results and Discussion|| |
Optimization of the extraction conditions
For sample extraction, different extraction methods (sonication or reflux), extraction solvents (various concentrations of aqueous methanol or aqueous ethanol), and extraction time (30, 40, or 60 min) were attempted [Table 4]. The highest yields of the selected components were obtained after 30 min of sonication in 50% aqueous methanol.
The calibration curve of each analyte displayed good linearity (R 2 > 0.9991) over a range of concentrations, LOD and LOQ were within the range 2.4-37.5 ng and 0.011-0.2582 μg [Table 2], respectively [Table 2]. The RSD values of the precision test were 0.16-1.97% for intraday assays and 0.39-2.06% for interday assays, while the RSD value for sample solution stability was <3.0% [Table 3]. The recovery of the method was in the range of 91.8-109.5%, with RSD <5.0% [Table 3]. The system was thus considered suitable for the chemical analysis of RMC and PMC.
Optimization of the high-performance liquid chromatography system and peak identification
A mixture of acetonitrile and 0.1% (v/v) formic acid in water was selected as mobile phase after several attempts using different combinations of acetonitrile (or methanol) and water, with or without acid (acetic acid, formic acid, and phosphoric acid). The detection wavelength and column temperature were set at 254 nm and 30°C, respectively. Under the optimized conditions, a good baseline, maximum number of detectable peaks, good resolution, and reasonable analytical time were warranted.
Ten batches each of the RMC and PMC were analyzed under the optimized LC-MS condition [Figure 2]. Eight marker components in the RMC and PMC samples were identified by HPLC-DAD-ESIMS analysis on the basis of retention time, ultraviolet absorption profile, and MS molecular ion in both positive and negative modes, with the respective reference standards [Table 5]. A reference chromatogram, each for RMC and PMC [Figure 2]a and b, was synthesized using the software of the Similarity Evaluation System for Chromatographic Fingerprint of TCM,  by combining the characteristic features in ten individual LC chromatograms of the samples. These two reference chromatograms (one for RMC and the other for PMC) were subsequently employed in the similarity test for comparison of retentions time and peak area for each sample [Table 1].
|Figure 2: (a) Fingerprints of ten batches of samples detected at ultraviolet 254 nm (A: Raw Moutan Cortex). (b) Fingerprints of 10 batches of samples detected at ultraviolet 254 nm (B: Processed Moutan Cortex)|
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Similarity test results showed that within the RMC and PMC groups, most samples displayed high similarity with the reference chromatogram, with similarity indices above 0.93 and 0.86, respectively. The high similarity among the samples indicated high degree of consistency of the samples, on the other hand, when the RMC sample (before processing) was compared to its PMC product (after processing), indices as low as 0.018 were obtained. The low similarity results indicated that significant differences existed before and after the processing procedures. In addition, when the major component signals were compared among the chromatograms, significant variations was detected [Figure 3]a and b. For examples, peaks 15-17 and 19 in the RMC samples disappeared in the PMC samples, with concomitant generation of new peaks (peaks 2-4, 6, 13, and 23) after the stir-frying process.
|Figure 3: The representative high-performance liquid chromatography chromatograms of Raw Moutan Cortex (a) (b) The representative high-performance liquid chromatography chromatograms of Processed Moutan Cortex (b) (c) The representative high-performance liquid chromatography chromatograms of mixed standards solution (c) (1) gallic acid; (2) 5-hydroxymethylfurfural; (7) catechin; (9) oxypaeoniflorin; (12) paeoniflorin; (19) quercetin; (20) benzoylpaeoniflorin; (22) paeonol|
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The contents of eight marker constituents were estimated in the RMC and PMC samples [Table 6]. The independent samples t-test results suggested that the average contents of six components, namely, quercetin, oxypaeoniflorin, paeoniflorin, benzoylpaeoniflorin, paeonol, and catechin decreased after the stir-frying processing (P < 0.05). On the other hand, the content of gallic acid was increased. 5-HMF, which was not detected in the RMC samples, could be identified in all PMC samples as a newly formed substance. Such chemical changes could be attributed to thermal decomposition during the process of high-temperature treatment. ,, It is noteworthy that the four compounds (paeoniflorin, benzoylpaeoniflorin, paeonol, and catechin) that undergo decomposition during the stir-frying procedure have been reported to be able to inhibit platelet aggregation, promote blood circulation, and remove blood stasis, , while gallic acid had hemostatic and astringent activities.  It can, therefore, be rationalized that the RMC is richer in the blood-activating components than the PMC, whereas PMC has higher contents of blood hemostatic components, thus leading to their different clinical properties.
|Table 6: Contents of identified components in RMC and PMC samples (mg/g)|
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Principal components analysis
PCA was employed to compare the difference between RMC and PMC, using the eight markers as variables and ten batches of samples as the observation object. According to the factor loading matrix calculation, the PCA loading plot [Figure 4] indicated that 5-HMF, gallic acid, and quercetin had great influence on the component 1, component 2, and component 3, respectively. The three-dimensional graphics of PCA scores [Figure 5] showed that RMC and PMC samples formed their own cluster within a small region apart from each other, indicating the two groups are distinctively different. Thus, the PCA analysis clearly supports the observation that the PMC samples had significantly different chemical composition than the RMC samples, which had not gone through the stir-drying process.
|Figure 4: Loading scatter three-dimensional plot of eight variables on three principal components. (a) Component1; (b) component2; (c) component3. (1) gallic acid; (2) 5-hydroxymethylfurfural; (7) catechin; (9) oxypaeoniflorin; (12) paeoniflorin; (19) quercetin; (20) benzoylpaeoniflorin; (22) paeonol|
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|Figure 5: Three-dimensional graphics of principal components analysis scores for Raw Moutan Cortex and Processed Moutan Cortex|
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During the PCA analysis, it was also noted that a small number of samples distributed outside the core of the clusters. For example, samples RMC-4 and RMC-5 were outliers of the RMC group, and PMC-11, PMC-14, and PMC-19 were outliers of the PMC group. Indeed, these samples displayed lower chromatographic similarity (relative to the reference chromatogram) than other samples in the same group [Table 1]. For samples RMC-4 and RMC-5, the low similarity may have been caused by the relatively low oxypaeoniflorin and quercetin contents, respectively. On the other hand, in PMC-11, PMC-14, and PMC-19 samples, the low similarity could be attributed to the high contents of 5-HMF and gallic acid in these samples. Such variations in the chemical composition may have arisen from environmental and/or growing conditions of the Paeonia plants in the field.
| Conclusion|| |
The raw and processed samples of Paeonia bark were compared in the present study on the basis of chemical fingerprinting analysis and simultaneous qualitative and quantitative analysis of the marker components. The results clearly showed significant changes in their chemical compositions before and after the stir-frying process. Chemical variation in the herbal materials may explain, at least partially, the different medicinal properties of RMC and PMC.
Financial support and sponsorship
This work was supported by the National Science Foundation of China (No. 81473352) and a Specialized Research Fund for TCM from the State Administration of Traditional Chinese Medicine of China (201207004-7).
Conflicts of interest
There are no conflicts of interest.
| References|| |
Zhao Z, Liang Z, Chan K, Lu G, Lee EL, Chen H, et al.
A unique issue in the standardization of Chinese materia medica: Processing. Planta Med 2010;76:1975-86.
Jin SY, Wang Q. Studies on Processing of Chinese Medicinal Yinpian and its Clinical Applications. Beijing: Chemical Industry Press; 2004.
Ye DJ, Yuan ST. Dictionary of Chinese Herbal Processing Science. Shanghai: Shanghai Science and Technology Press; 2005.
Cai BC, Gong QF. Processing of Chinese Medicinal Herbs. Beijing: People Health Press; 2009.
Ding AW. Chinese Materia Medica Preparation. Beijing: Higher Education Press; 2007.
The Pharmacopoeia Committee of China. Pharmacopoeia of the People's Republic of China. Beijing: Chemical Industry Press; 2010.
Qiu ZC, Chen YX, Zhou RL. Effects of different processing on the pharmacodynamic actions of Cortex Moutan. Prog Mod Biomed 2009;9:2882-5.
Li X, Zhang L, Ding AW. Study on the hemostatic pharmacodynamics of Cortex Moutan Carbonisata. Chin Trad Pat Med 2010;32:129-30.
Ishida H, Takamatsu M, Tsuji K, Kosuge T. Studies on active substances in herbs used for oketsu ("stagnant blood") in Chinese medicine. VI. On the anticoagulative principle in paeoniae radix. Chem Pharm Bull (Tokyo) 1987;35:849-52.
Fan X, Wang Z, Li Q, Ma T, Bi K, Jia Y. UPLC characteristic chromatographic profile of Moutan Cortex. Zhongguo Zhong Yao Za Zhi 2011;36:715-7.
Yoshikawa M, Uchida E, Kawaguchi A, Kitagawa I, Yamahara J. Galloyl-oxypaeoniflorin, suffruticosides A, B, C, and D, five new antioxidative glycosides, and suffruticoside E, A paeonol glycoside, from Chinese moutan cortex. Chem Pharm Bull (Tokyo) 1992;40:2248-50.
Kwon OG, Kim SH, Chun BY, Park CK, Son KH. Isolation of antimicrobial components from Moutan Cortex. Korean J Pharmacogn 1999;30:340-4.
Kroes BH, van den Berg AJ, Quarles van Ufford HC, van Dijk H, Labadie RP. Anti-inflammatory activity of gallic acid. Planta Med 1992;58:499-504.
Koo YK, Kim JM, Koo JY, Kang SS, Bae K, Kim YS, et al.
Platelet anti-aggregatory and blood anti-coagulant effects of compounds isolated from Paeonia lactiflora
and Paeonia suffruticosa
. Pharmazie 2010;65:624-8.
Hu S, Shen G, Zhao W, Wang F, Jiang X, Huang D. Paeonol, the main active principles of Paeonia moutan
, ameliorates alcoholic steatohepatitis in mice. J Ethnopharmacol 2010;128:100-6.
Ding A, Guo R, Rong J. Determination of paeonol in carbonized bark of Paeonia suffruticosa
Andr. by HPLC. Zhongguo Zhong Yao Za Zhi 1996;21:23-4, 63.
Fang XC, An DK, Dong SS, Shen ZJ. Analysis of paeonol in moutan cortex and in Liuwei Dihuang pill with direct evaporation - High resolution gas chromatography. J China Pharm Univ 1989;2:109-11.
Yang GL, Song XR, Zhang HY, Wang DX, Sun HW. Determination of paeonol and paeoniflorin in Moutan cortex with micelle capillary electrophoresis. Chin J Anal Chem 1999;27:1-4.
Yu K, Wang YW, Cheng YY. Determination of paeonol and paeoniflorin in Chinese medicine Cortex Moutan and 'Shuangdan' granule by micellar electrokinetic capillary chromatography. J Pharm Biomes Anal 2006;40:1257-62.
Zhao XF, Wang W, Sun LQ. Capillary zone electrophoresis method for the determination of paeoniflorin in Paeonia suffruticosa
. China Trad Herb Drugs 2003;3:271-2.
Song Y, He Q, Li P, Cheng YY. Capillary high performance liquid chromatography coupled with electrospray ionization mass spectrometry for rapid analysis of pinane monoterpene glycosides in Cortex Moutan. J Sep Sci 2008;31:64-70.
Wu YT, Huang WY, Lin TC, Sheu SJ. Determination of moutan tannins by high-performance liquid chromatography and capillary electrophoresis. J Sep Sci 2003;26:1629-34.
Chen G, Zhang L, Zhu Y. Determination of glycosides and sugars in Moutan Cortex by capillary electrophoresis with electrochemical detection. J Pharm Biomed Anal 2006;41:129-34.
Ding Y, Wu EQ, Chen JB, Nguyen HT, Do TH, Park KL, et al
. Quality evaluation of Moutan Cortex radicis using multiple component analysis by high performance liquid chromatography. Bull Korean Chem Soc 2009;30:2240-4.
He Q, Ge ZW, Song Y, Cheng YY. Quality evaluation of cortex moutan by high performance liquid chromatography coupled with diode array detector and electrospary ionization tandem mass spectrometry. Chem Pharm Bull (Tokyo) 2006;54:1271-5.
Zhou G, Lv QH. Studies on content determination and fingerprint of active constituents in different parts of cortex moutan. Zhongguo Zhong Yao Za Zhi 2008;33:2070-3.
Wu MZ, He Y, Tang LY, Wang ZJ. Clustering analysis of HPLC chromatographic fingerprints of Paeonia suffruticosa. Zhongguo Zhong Yao Za Zhi 2007;32:1054-6.
Gao XB, Sun L, Qiao SY, Gao S, Che YZ. HPLC fingerprint of Liuwei Dihuang condensed pills. China Trad Herb Drugs 2013;7:900-4.
Xu SJ, Yang L, Zeng X, Zhang M, Wang ZT. Characterization of compounds in the Chinese herbal drug Mu-Dan-Pi by liquid chromatography coupled to electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom 2006;20:3275-88.
Xiao C, Wu M, Chen Y, Zhang Y, Zhao X, Zheng X. Revealing metabolomic variations in Cortex Moutan from different root parts using HPLC-MS method. Phytochem Anal 2015;26:86-93.
Zhao WJ, Lin Y, Li PF, Liu Y. Analysis of chemical composition in cortex moutan with HPLC - QTOFMS. J Pharm Pract 2014;4:261-5.
Drug administration of Beijing Municipal. Rules for the Processing of Traditional Chinese Medicine. Beijing: Chemical Industry Publishing Press; 2010.
Zhuo HX, Yang Z, Pei C, Pu J, Jin Y, Liang LY. Chromatographic fingerprint analysis and rutin and quercetin compositions in the leaf and whole-plant samples of di- and tetraploid Gynostemma pentaphyllum
. J Agric Food Chem 2011;59:3042-9.
Geng F, Wang XJ. 5-hydroxymethyl-2-furfural (5-HMF) in traditional Chinese medicines. Sci World Technol Mod Trad Chin Med 2005;7:52-6.
Ding X, Cai BC. Comparison of the active contents of Cornus officinalis
and the processed products.Chin Trad Pat Med 2006;28:1597-600.
Yamaguchi S, Kawamura T, Noro Y, Kawamura K, Tanaka T. Drying method of medicinal plants for the quality control of crude drugs (1). Numerical evaluation of surface color and commercial value. Nat Med 2001;55:139-42.
Lin Z, Fang Y, Huang A, Chen L, Guo S, Chen J. Chemical constituents from Sedum aizoon and their hemostatic activity. Pharm Biol 2014;52:1429-34.
| Authors|| |
Jiang Meng, Work at Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, China. Engage in the research of Chinese herbal medicine processing and the quality standard of decoction pieces.
Bin Zhao, Work at Department of Pharmacognosy , Zhongshan Torch Polytechnic, China. Engage in health products research and development and quality standard of Chinese medicine research.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6]
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