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Year : 2016  |  Volume : 12  |  Issue : 45  |  Page : 27-31  

Potential antidepressant constituents of Nigella sativa seeds

1 Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt
2 Department of Pharmacology, College of Medicine, Qassim University, Buraidah, AL-Qassim, Saudi Arabia
3 Department of Pharmacology, Faculty of Veterinary Medicine, Alexandria University, Egypt; Department of Veterinary Medicine, College of Agricultural and Veterinary Medicine, Qassim University, Buraidah, AL-Qassim, Saudi Arabia
4 Department of Pharmacology, Faculty of Veterinary Medicine, Alexandria University; Department of Food Hygiene, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt

Date of Web Publication10-Feb-2016

Correspondence Address:
Ehab S Elkhayat
Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut 71524
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1296.176118

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Background: Nigella sativa Linn. is well known seed in the Middle East, Asia, and the Far East as a natural remedy for many ailments and as a flavoring agent proclaimed medicinal usage dating back to the ancient Egyptians, Greeks, and Romans. An authentic saying of the Prophet Muhammad (Peace Be Upon Him) about black seed is also quoted in Al-Bukhari. Objective: This study was carried out to evaluate the antidepressant effect and isolate the potential antidepressant constituents of the polar extract of N. sativa seeds. Materials and Methods: The antidepressant effect was evaluated through the immobility duration in tail suspension and forced swim tests (FSTs). Albino mice were orally treated with N. sativa polar extract and its RP-18 column chromatography fractions (50 and 100 mg/kg,). Results: The polar extract and two of its sub-fractions were significantly able to decrease the immobility time of mice when subjected to both tail suspension and FSTs, the effects are comparable to standard drug (Sertraline, 5 mg/kg). However, these treatments did not affect the number of crossings and rearing in the open field test. Phytochemical investigation of the two active fractions led to the isolation of quercetin-3-O-α-L-rhamnopyranoside 1, quercetin-7-O-β-D-gluco- pyranoside 2, tauroside E 3, and sapindoside B as the potential antidepressant constituents.

Keywords: Antidepressant constituents, forced swim test, Nigella sativa, tail suspension test

How to cite this article:
Elkhayat ES, Alorainy MS, El-Ashmawy IM, Fat'hi S. Potential antidepressant constituents of Nigella sativa seeds. Phcog Mag 2016;12, Suppl S1:27-31

How to cite this URL:
Elkhayat ES, Alorainy MS, El-Ashmawy IM, Fat'hi S. Potential antidepressant constituents of Nigella sativa seeds. Phcog Mag [serial online] 2016 [cited 2022 Nov 28];12, Suppl S1:27-31. Available from: http://www.phcog.com/text.asp?2016/12/45/27/176118


  • Phytochemical and biological evaluation the antidepressant constituents in Nigella sativa using the tail suspension and forced swim methods afforded the isolation and identification of quercetin-3-O-α-L rhamnopyranoside, quercetin-7-O-β-D gluco pyranoside, tauroside E, and sapindoside B as the potential antidepressant constituents in the polar extract of N. sativa. The isolated compounds were identified through extensive NMR analysis (1D, 2D, ESI MS).

   Introduction Top

Most people feel anxious or depressed at times. Many difficult situations can lead a person to feel sad, lonely, scared, nervous, or anxious. These feelings are normal reactions to life's stressors. However some people experience these feelings daily or nearly daily for no apparent reason, making it difficult to carry on with normal life. These people may have an anxiety disorder, depression, or both.[1],[2] Depression is a common disorder of the modern life. There are more than 350 million people of all ages suffer from depression worldwide. It was estimated that 1 million suicides occur due to depression every year.[3] Despite the availability of antidepressant drugs as; tricyclic antidepressants, selective reversible inhibitors of monoamine oxidase-A, or selective serotonin reuptake inhibitors (SSRIs), approximately 50% of the people experiencing depression not receiving any treatment.[4] Although the vast majority of the drugs used in treating depression are synthetic, they have various adverse effects ranging from drowsiness and ataxia (benzodiazepines) to insomnia and libido (selective SSRIs). Drugs of natural sources seem to have fewer adverse effects while having the curing ability in the same way as their synthetic equivalents. Recently, the search for novel drugs from medicinal plants for the psychiatric disorder has stepped forward significantly disclosing promising pharmacological efficiency of a variety of plants used in complementary and alternative medicines for management of mood disorders.[5]

In view of the previously mentioned, the seeds of Nigella sativa were selected to evaluate their potential antidepressant constituents. N. sativa have been shown to be rich in diverse phytoconstituents including volatile and fixed oils, monoterpenes, alkaloids, triterpenes, and saponins.[6],[7],[8],[9],[10],[11] In addition to the wide spectrum of activity, such as antioxidant, antitumor, antiinflammatory, analgesic, antiaflatoxin, smooth muscle relaxant, cytotoxic, melanogenic, immunostimulant, and antidiabetic activities.[12],[13],[14],[15],[16],[17],[18],[19],[20] However, previous studies showed that the aqueous extract of N. sativa, fixed and volatile oils produced antianxiety effect,[21],[22] in this paper we try to explore the major constituents responsible for the antidepressant effect in the polar extract.

   Materials and Methods Top

Plant material

The seeds of N. sativa L. were purchased from the local herbal market in Makah.

Chemicals and instruments

Sertraline hydrochloride (Modapex®, APEX Pharmaceuticals, Egypt) was used as a reference standard for antidepressant activity. Vacuum liquid chromatography (VLC) and column chromatography were carried out using silica gel 60, 0.04–0.063 mm mesh size (Merck). Reversed phase silica gel for column chromatography, RP-18 (0.04–0.063 mm Merck). Sephadex LH-20 (0.25–0.1 mm, Merck). Precoated silica gel 60 F254 plates (Merck) were used for thin layer chromatography (TLC). The TLC plates were visualized by spraying with p-anisaldehyde/H2 SO4 reagent and heating at 110°C for 1–2 min.1 H and 13 C-NMR spectra were recorded on a JEOL-JNM-EX-400 spectrometer (400 MHz for 1 H and 100 MHz for 13 C). Mass spectra electrospray ionization mass spectrometry (ESI-MS) were recorded on a Finnigan MAT TSQ-7000 triple stage quadrupole mass spectrometer. All solvents for extraction and isolation were distilled prior to use. NMR grade solvents (Merck) were used for NMR analysis.

Extraction and isolation

The evaluation done by Raza et al. showed that the aqueous extract had high antidepressant activity. Thus, the study was planned to use the bioassay-guided fractionation for the polar extract of N. sativa seeds in order to isolate and identify the potential antidepressant constituents. N. sativa seeds (300 g) were crushed into fine powder and were extracted with chloroform, then dried, and extracted with 70% MeOH (3 L × 1 L) at room temperature till exhaustion to get the polar extract. The combined methanol extract was filtered and then evaporated under reduced pressure to get a dark brown oily residue of the polar extract (19.1 g). The preliminary assay using the forced swimming model (FSM) and tail suspension test (TST) confirmed the antidepressant–like activity of the extract. Then the dried extract was subjected to chromatographic fractionation using RP-18 column (0.04–0.063 mm; 750 mm × 35 mm, i.d.) eluted sequentially with H2O, H2O/MeOH (8:2, 1:1, 3:7) and finally with MeOH 100% to get five fractions N1-5, the TLC analysis revealed similarity in the composition of fractions N-1 and 2, thus N-1 and 2 were combined into one fraction (N-1). The preliminary antidepressant assay using FSM and TST revealed that only fractions N-3 and N-4 have antidepressant–like activity. Thus, these two fractions were subjected for the phytochemical investigation to isolate and identify the potential antidepressant components.

Isolation of compounds from fraction N-3

Fraction N-3 was subjected to RP-18 column chromatography (0.04–0.063 mm; 500 mm × 25 mm, i.d.) eluted with H2O/MeOH (4:6). The TLC analysis of the eluents combined them into 4 sub-fractions (N-3a – N-3d). TLC analysis of sub-fraction N-3c showed a major component (CHCl3/MeOH/H2O 65:30:5), which was isolated after silica gel column chromatography eluted with CHCl3/MeOH/H2O (65:25:4) to get compound 1 (Quercetin-3-O-α-L-rhamnopyranoside).[23] Sub-fraction N-3b was subjected to repeated silica gel column chromatography eluted with CHCl3/MeOH/H2O (60:30:5) to get compound 2 (Quercetin-7-O- β-D-glucopyaranoside).[24]

Isolation of compounds from fraction N-4

Fraction N-4 was subjected to VLC, on silica gel and eluted successively with CHCl3/MeOH gradients (85:15); (7:3); (1:1) and MeOH 100%, to get four sub-fractions. The TLC analysis (CHCl3/MeOH/H2O, 65:30:5), showed the similarity of the main composition of the obtained sub-fractions; hence they were combined in two sub-fractions N-4a and N-4b. VLC on silica gel eluted CHCl3/MeOH gradients of sub-fraction N-4a afforded five fractions, fraction N4-a3 was further purified by silica gel column chromatography (700 mm × 35 mm, i.d.), eluted with CHCl3/MeOH 8:2 to get compound 1 (Tauroside E).[25] Sub-fraction N-4b was passed through Sephadex LH-20 column (750 mm × 30 mm, i.d.) eluted with MeOH, followed by silica gel column chromatography eluted with CHCl3/EtOAC gradient to get compounds 2 (Sapindoside B).[26]

Structure elucidation of the isolated components 1–4

Elucidation of the chemical structures of the isolated compounds [Figure 1], was based on one-dimensional (1D) and 2D-NMR spectroscopic analysis (1 H,13 C-NMR, HMBC, and COSY), in conjunction with ESI-MS and comparison with compounds' previously published data.
Figure 1: Structures of the isolated compounds

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This study was approved by the Ethics Committee. All experiments were performed in accordance with Good Clinical Practice, as revealed by Helsinki Declaration and approved by the World Health Association; the guidelines were adhered during the whole experiment.

Swiss albino mice of either sex weighing between 20 and 25 g were used for the study. The animals were maintained under standard environmental conditions (25°C ± 2°C and relative humidity of 45–55%) and were fed with standard pellet diet and water were provided ad libitum, animals observed daily for signs of toxicity and behavioral changes. All experiments were carried out between 10:00 and 17:00 h.

Drug administration

Animals were distributed randomly on experiment day into control and experimental groups; each group is six animals. Tested materials were dissolved in distilled water to the desired dosages; animals of the control group received distilled water. Solutions of the standard drug, extract and tested fractions were administered orally via gastric intubation 1 h before experiment in case of acute study, and administration once a day at 10.00 am for 21 days in case of subchronic study. The animals were acclimatized 1 h before behavioral tests. Before running the TST and forced swim tests (FST) experiments, animals were observed in the open field test (OFT) in order to exclude the influence of motor alterations on the animal performance.

Acute toxicity study

Mice were fasted for 3 h prior to the experiment. An acute toxicity study was carried out by giving 4 doses (0.25, 0.50, 0.75 and 1.00 g/kg) of the extract to different mice groups (n = 5). The mortality and general behavior of the mice were observed for 48 h with special attention to the first 30 min and the first 4 h after the single oral administration then periodically during the 48 h and daily for a total of 2 weeks.

Open field test

The OFT was used as a complement for the TST and FST to reject nonspecific responses of the antidepressant materials. The OFT was carried out in a glass container (transparent walls and dark floor, 40 cm × 40 cm × 25 cm), subdivided into 10 equal areas. Respective treatment was given to the animals 60 min before the experiment and the animals were individually placed in the center of the open field and allowed to explore it freely. The following parameters were observed for the last 10 min of a 20 min testing period; Ambulation (number of squares crossed with all 4 paws) and number of grooming and rearing.[27] The open field was cleaned carefully with 70% alcohol between tests to eliminate odors. Mice were divided equally into four groups (n = 6), and treated for 3 weeks as follow, the first group given distilled water (10 ml/kg, control), the second and third groups given the polar extract 100 and 200 mg/kg respectively, and the fourth group was given sertraline (5 mg/kg).

Tail suspension test

TST was performed according to the method described by Steru et al.[28],[29] The test was conducted an hour after the last treatment in the dim lighted room. Animals were individually suspended by the tail using adhesive plaster 1 cm from the tip to the edge of a [table 50] cm from the floor. The suspension was continued for 6 min, the total period of immobility was recorded during the last 4 min. The animal was considered to be immobile when it did not make any struggling movement, any attempt to catch the adhesive tape, or body torsions.

Forced swim test

The test was carried out as proposed by Porsolt et al.[30],[31],[32] The test was conducted an hour after the last treatment in the dim lighted room. Mouse was individually forced to swim in open, transparent glass chamber (45 cm × 45 cm × 25 cm) containing fresh water to a height of 15 cm and maintained at 25°C ± 2°C. At this height of water, animals were not able to support themselves by touching the bottom or the side walls of the chamber with their hind-paws or tail. Water in the chamber was changed after each animal. Duration of immobility was manually recorded during the last 4 min of 6 min testing period. The mouse was considered immobile when it ceased struggling and remained floating motionless on water, making only those movements necessary to keep their head above water.

Statistical analysis

All data were expressed as a mean ± standard error of the mean. Normal distribution of data was analyzed using the Student's t-test. The statistical significance was evaluated by one-way analysis of variance. The values were considered to be statistically significant different when P < 0.05.

   Results Top

Biologically guided isolation

Despite the previous results which conferred antidepressant effect of the aqueous extract and of N. sativa,[22] there was no phytochemical investigation concerned with the nature of compounds in this extract. This work was aimed to evaluate the antidepressant–like effect the polar extract of N. sativa, as well as isolation and identification of the potential antidepressant components. The antidepressant assay using the TST and FST methods demonstrated that the polar extract of N. sativa in mice was able to induce antidepressant–like effect at dose levels of 100 and 200 mg/kg, where it showed immobility time reduction of 28.3 and 33.8% in the TST and 22.5 and 31.3% in the FST, respectively [Table 1] and [Table 2]. Chromatographic fractionation of the polar extract on RP-18 to yield 4 fractions (N-1 to 4), on evaluation of the antidepressant activities of the obtained fractions using the TST and FST experiments, it was evident that the antidepressant effect was restricted to fractions N-3 and 4, that could attributed to the type chemical constituents in these fractions. TLC analysis of the active factions (N-3 and N-4) revealed two major constituents in each of them. Therefore, N-3 and N-4 were individually subjected to further phytochemical investigations using repeated column chromatography in order to isolate the potential antidepressant constituents. Quercetin-3-O-α-L-rhamnopyranoside (1), and Quercetin-7-O-β-D-glucopyaranoside (2), were isolated from N-3, and tauroside E (3), and sapindoside B (4) were isolated from N-4. The isolated compounds were unambiguously identified through extensive NMR analysis (1D, 2D, ESI-MS) and comparison with previously published data.

Effect of the polar extract and fractions N-3 and 4 on the immobility period in forced swim tests and tail suspension test

Results showed that both the acute and subchronic administration [Table 1] and [Table 2] of the polar extract, as well as fractions 3 and 4 (50 and 100 mg/kg) produced a significant dose dependant reduction in the immobility time in both forced swimming and TSTs comparable to sertraline (5 mg/kg), with the highest antidepressant–like activity at dose of 100 mg/kg. These treatments did not affect the number of crossing and rearing in the OFT.
Table 1: Effect of oral administration of different fractions of Nigella sativa on immobility time in the TST

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Table 2: Effect of oral administration of different fractions of Nigella sativa on immobility time in the FST

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   Discussion Top

Depression is a neurological disorder that becomes more prevalent in recent decades [33] Due to the complexity of depression mechanism, many currently available synthetic antidepressants show low therapeutic response and even more adverse effects.[34] Therefore, there is an exaggerated need for more effective antidepressants with a lower adverse effects. Many medicinal plants showed antidepressant–like effects, such as Hypericum perforatum,[35]Passiflora edulis,[36]Plantago asiatica,[37] and Ginko biloba.[38] In addition, certain plant constituents have been linked to attenuation of depression including flavonoids, oligo- and poly-saccharides, alkaloids, and terpenoids.[39] This study aimed to isolate and identify the potential antidepressant constituents in N. sativa seeds using the FST and TST experiments as behavioral model tests.

Effect on immobility times in tail suspension test and forced swim tests experiments

Evaluation of the antidepressant–like effect was based on the most widely used behavioral animal models that practiced the clinical efficacy of many antidepressant treatments which are therapeutically effective in human depression; TST and the FST.[30],[31],[32],[33] The polar extract of N. sativa and two of its yielded chromatographic fractions (N-3 and N-4) exhibited antidepressant–like effect as shown in [Table 1] and [Table 2]. In order to detect any association between immobility in the tests with increase in locomotor activity, repeated administration of the tested materials did not increase the locomotor activity in the open field at the doses which showed the antidepressant–like effect, revealing that the effect of the tested materials is a consequence of antidepressant activity.

The phytochemical investigations of the active factions N-3 and N-4 afforded the isolation of 2 flavonoid glycosides and two triterpenoidal glycosides, respectively. Thus, it seems that the antidepressant–like effect of the N. sativa polar extract is attributed to the presence of flavonoid and triterpene phytoconstituents. It was reported that triterpenoids exert antidepressant–like effect by inhibiting the uptake of norepinephrine, serotonin, and dopamine.[40],[41] Meanwhile, flavonoids elicit the antidepressant–like effect through antioxidant protection against lipid peroxidation that leads to increase of 5-hydroxytryptamine and norepinephrine in the central nervous system.[34] Moreover, Zheng et al., reported that the flavonid extract of Apocynum venetum have reduced the immobility time in TST and FST showing antidepressant–like effect through increase of norepinephrin and dopamine along with their metabolites in the mice hippocampus.[42]

   Conclusion Top

This work confirms the antidepressant–like activity of the polar extract of N. sativa seeds, referring this activity to the presence of triterpenoid and flavonoid phytoconstituents.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

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   Authors Top

Dr. Ehab Saad Elkhayat, Associate professor of natural products and plant chemistry in Faculty of pharmacy, Al-Azhar University, Assiut, Egypt. Scientific Interests: Bioactive natural products, marine natural products, phytochemistry and special interest in endophytic fungi.


  [Figure 1]

  [Table 1], [Table 2]

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