| ORIGINAL ARTICLE |
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| Year : 2015 | Volume
: 11
| Issue : 43 | Page : 546-554 |
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Microwave assisted extraction of polysaccharides from Yupingfeng powder and their antioxidant activity
Dan Wang1, Bi-Bo Zhang1, Xiao-Xia Qu1, Feng Gao1, Min-Yong Yuan2
1 Department of Medicinal Plants, Agronomy College, Sichuan Agricultural University, Wenjiang Region, Chengdu 611130, P. R. China
2 Department of Pharmacy, The First Affiliated Hospital of Chengdu Medical College, Xindu Region, Chengdu 610500, P. R. China
Correspondence Address:
Feng Gao
Department of Medicinal Plants, Agronomy College, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang Region, Chengdu 611130
P. R. China
 Source of Support: This research was funded by NSFC (No.81001383) and Applied Basic Research Programs of Science and Technology Department of Sichuan Province (No. 2012JY0035), Conflict of Interest: None declared.  | Check |
DOI: 10.4103/0973-1296.160468
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Background: Microwave assisted reflux extraction of polysaccharides YPF-P from the famous Chinese traditional drug, Yupingfeng powder, optimization of extracting conditions and evaluation of their antioxidant activity were conducted in this study. Results: Single factor effect trends were achieved through yields and contends of YPF P obtained from different extracting conditions. Then through a three level, four variable Box Behnken design of response surface methodology adopting yield as response, the optimal conditions were determined as follows: Material/solvent ratio 1:23.37, microwave power 560 W, Extraction temperature 64°C, and extraction time 9.62 min. Under the optimal conditions, the YPF P extraction yield was 3.23%, and its content was detected as 38.52%. In antioxidant assays, the YPF P was tested to possess 1,1 diphenyl 2 picrylhydrazyl (DPPH) radical scavenging activities with an IC50 value of 0.262 mg/ml. In addition, YPF P was also proved to have relatively low ferric reducing antioxidant power (FRAP), compared to Vc, through FRAP assay. Conclusion: In the microwave assisted reflux extraction research, good YPF P yield was achieved from materials with relatively low YPF P content. And for the first time, both DPPH and FRAP assays were conducted on YPF P, which proved that the antioxidant activity of YPF P contributed to the functions of this medicine.
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