Anti-aging effect of polysaccharide from Bletilla striata on nematode Caenorhabditis elegans
Yusi Zhang1, Ting Lv1, Min Li2, Ting Xue2, Hui Liu2, Weiming Zhang1, Xiaoyu Ding1, Ziheng Zhuang1
1 College of Life Sciences, Nanjing Normal University, Nanjing, 210046, China
2 School of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, 213164, China
|Date of Submission||03-Jun-2014|
|Date of Decision||19-Jun-2014|
|Date of Web Publication||10-Jul-2015|
College of Life Sciences, Nanjing Normal University, Nanjing, 210046
School of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, 213164
Source of Support: Nil, Conflict of Interest: None declared.
| Abstract|| |
Background: Polysaccharide isolated from Bletilla striata, a well known traditional Chinese medicine (Bletilla striata polysaccharide [BSP]) has been found to play important roles in endothelial cells proliferation, inducible nitric oxide stimulation, wound healing acceleration and other processes. Recent studies found that B. striata has anti oxidative properties, however, potential anti aging effects of BSP in whole organisms has not been characterized. Objective: To investigate whether BSP has anti aging effects on Caenorhabditis elegans. Materials and Methods: After treatment with BSP, the lifespan, locomotion ability, and stress resistance of C. elegans was determined. To provide insight into the underlying mechanism for the anti aging effect of BSP, we measured its effect on bacterial growth, brood size of C. elegans, and the insulin/insulin like growth factor (IGF) signaling pathway. Results: After BSP treatment, the lifespan of C. elegans was extended, and its locomotion ability and stress resistance were increased. BSP was found to have no effect on bacterial growth or on reproduction of C. elegans, However, mRNA levels of age-1 and hcf-1 were reduced after BSP treatment. Additionally, we observed that BSP did not extend the lifespan of daf 16 mutant animals. Conclusion: BSP produces an anti aging effect on C. elegans through the insulin/IGF signaling pathway and holds promise for future development as a functional food.
Keywords: Anti-aging, Bletilla striata polysaccharide, Caenorhabditis elegans, lifespan, stress resistance
|How to cite this article:|
Zhang Y, Lv T, Li M, Xue T, Liu H, Zhang W, Ding X, Zhuang Z. Anti-aging effect of polysaccharide from Bletilla striata on nematode Caenorhabditis elegans. Phcog Mag 2015;11:449-54
|How to cite this URL:|
Zhang Y, Lv T, Li M, Xue T, Liu H, Zhang W, Ding X, Zhuang Z. Anti-aging effect of polysaccharide from Bletilla striata on nematode Caenorhabditis elegans. Phcog Mag [serial online] 2015 [cited 2021 May 16];11:449-54. Available from: http://www.phcog.com/text.asp?2015/11/43/449/160447
| Introduction|| |
Bletilla striata is a famous traditional Chinese medicine that is widely used in the treatment of ulcers, bone injuries, acne and many other diseases in China. Many compounds have been isolated from B. striata such as bibenzyls, phenanthrenes, phenolic acid, and polysaccharide., Among these compounds, polysaccharide isolated from B. striata has been found to have many functions. Bletilla striata polysaccharide (BSP) has been found to induce endothelial cells proliferation and vascular endothelial growth factor expression. BSP has also been found to stimulate inducible nitric oxide synthase and proinflammatory cytokine expression in macrophages. Hydrogel prepared from BSP has potential wound healing effects and has been demonstrated to accelerate wound closure in a full thickness trauma mouse model. In addition to its direct medicinal functions, BSP has been successfully used in the delivery of drugs such as oligonucleotide and antibiotics.,
Recent studies found that B. striata has anti oxidative effect. This anti oxidative ability was first demonstrated through DPPH radical scavenging activity assay and ferric reducing antioxidant power assay. The anti oxidative ability was further confirmed by measuring reactive oxygen species levels in response to H2O2 in the HepG2 cell line. This property has been demonstrated for both the B. striata extract as well as polysaccharide isolated from B. striata. Many drugs with the anti oxidative ability have been found to have anti aging effects in organisms. Therefore, we wanted to test if BSP similarly exhibits anti aging effects. For these studies, we used the model organism Caenorhabditis elegans, widely used in aging mechanism research and anti aging drug discovery due to its relatively short lifespan and conserved mechanisms for regulation of aging.
| Materials and methods|| |
Preparation of Bletilla striata polysaccharide
Bletilla striata polysaccharide was prepared and stored in our lab using a protocol described previously. Briefly, homogenized, dry B. striata was dispersed in hot distilled water for 4 h and then filtered. Polysaccharide was then precipitated using 3 volume of 95% ethanol as crude extract. Proteins were removed from this extract using the Sevag method. The crude extract was then purified by DEAE Cellulose column and Sephadex G 100 column to result in a single peak corresponding to purified BSP extract.
Worm strain maintenance
The strains used in this study were wild type N2 and mutant strain daf 16 (obtained from the Caenorhabditis Genetics Center). Nematodes were maintained on nematode growth medium (NGM) plates seeded with Escherichia coli OP50 at 20°C as described. Age synchronous populations of C. elegans were obtained as described previously. BSP was added to the NGM plates just before plating.
Lifespan assay and reproduction
The lifespan assay was performed as described. BSP treatment was performed throughout the lifespan from the L4 larvae stage. During the lifespan assay, the worms were transferred daily for the first several days of adulthood. The surviving nematodes were measured, and recorded every day and worms were scored as “dead” when they did not respond to the stimulation of a platinum wire. The results showed are representative of at least three trials.
Reproduction was assessed by the brood size, which was determined as the number of offspring at all stages beyond the egg. Ten replicates were performed.
For the locomotion behavior assay, BSP treatment was performed throughout the lifespan from L4 larvae. Head thrash and body bend were used as endpoints for locomotion behavior. Head thrashes are defined as a change in the direction of bending at the mid body. Body bends are defined as a change in the direction of the part of nematodes corresponding to the posterior bulb of the pharynx along the y axis, assuming that nematode was traveling along the x axis.
During the locomotion behavior assay, the examined nematodes were transferred into the assay plate containing K medium on top of the agar. After a recovery time of 1 min, head thrashes, and body bends were counted for 1 min and 20 s respectively. Twenty replicates were performed for each experiment.
Stress resistance assays
To assay thermal stress resistance, worms pre treated with BSP for 48 hr were transferred to the 35°C condition, and then the lifespan was measured and analyzed as described above. To assay oxidative stress resistance, worms pre treated with BSP for 48 hr were transferred to medium containing paraquat, and then the lifespan was measured and analyzed as described above.
Bacterial growth assay
Escherichia coli OP50 was seeded into sterilized liquid medium with or without 50 μg/ml BSP, and initial OD595 values were measured against sterile medium. Samples were transferred to rocking shaker at 37°C, and OD595 values were measured for 10 h.
RNA isolation and Quantitative real time polymerase chain reaction
Total RNA was isolated using RNAiso Plus (Takara) from worms treated with or without 50 μg/ml of BSP for 48 h. Total RNA was then reverse transcribed using PrimeScript 1st strand cDNA synthesis kit (Takara). Quantitative real time polymerase chain reaction (RT PCR) was used to determine the relative quantification of the targeted genes in comparison to the reference act 1 gene, and the results were expressed as the relative expression ratio (between targeted gene and internal control act 1).The primers used in this study were as follows: Daf 2, forward 5’ - CCAACCGAACGGAGACCT-3’, reverse 5’- CGATAGCCGAACACGAAC-3’; age 1, forward 5’ - AATGGCAAAGGATCGCTG-3’, reverse 5’- GGAGTTTCGTTCGGATTG-3’; daf 16, forward 5’- CGTTTCCTTCGGATTTCA-3’, reverse 5’- ATTCCTTCCTGGCTTTGC-3’; hcf 1, forward 5’- CGGAAGGCTTGGAGTAAC-3’, reverse 5’- ATGGTTGCTTGCGAGGAG-3’ ; act 1, forward 5’- TGACGGACAAGTCATCACCG-3’, reverse 5’- CATGGTGGTTCCTCCGGAAA-3’.
Data are presented as means ± standard error of the mean. Graphs were generated using Microsoft Excel (Microsoft Corp., Redmond, WA). Statistical analysis was performed using SPSS 12.0 (SPSS Inc., Chicago, USA). Differences between groups were determined using analysis of variance. Probability levels of 0.05 and 0.01 were considered statistically significant. The lifespan data were statistically analyzed using a 2 tailed, 2 sample t test (Minitab Ltd., Coventry, UK).
| Results|| |
Bletilla striata polysaccharide extends lifespan of Caenorhabditis elegans
Nematodes were treated with different doses of BSP from L4 larvae stage in order to investigate BSP’s effect on lifespan of C. elegans. As shown in [[Figure 1]a] and [[Figure 1]b], all three concentrations of BSP tested showed extended lifespan for C. elegans and the best lifespan extending effect was observed in worms treated with 50 μg/ml of BSP.
|Figure 1: Bletilla striata polysaccharide (BSP) extends lifespan of Caenorhabditis elegans. (a) Survival curves of C. elegans treated with different dose of BSP. (b) Comparison of mean lifespans in nematodes treated with different dose of BSP (*P < 0.05)|
Click here to view
Bletilla striata polysaccharide improves the locomotion behavior of Caenorhabditis elegans
Caenorhabditis elegans shows gradually impaired locomotion ability during its aging process especially at the last stage. We next investigated the effect of BSP on the locomotion ability of C. elegans. Two important endpoints for locomotion ability–head thrash and body bend of C. elegans were recorded every 4 days during its lifespan. As shown in [[Figure 2]a] and [[Figure 2]b], the C. elegans treated with BSP showed significantly enhanced locomotion ability during its aging process compared with untreated animals, indicating that BSP treatment enhances the locomotion behavior of C. elegans and it can improve the life quality of aged nematodes.
|Figure 2: Bletilla striata polysaccharide (BSP) improves the locomotion behavior of Caenorhabditis elegans. (a) Body bends of C. elegans treated with 50 ug/ml of BSP during its lifespan. (b) Head thrashes of C. elegans treated with 50 ug/ml of BSP during its lifespan (**P < 0.01)|
Click here to view
Bletilla striata polysaccharide improves the stress resistance of Caenorhabditis elegans
We next investigated the effect of BSP on the stress resistance of C. elegans as many drugs with anti aging effect affects the survival of animals under stress conditions. Nematodes pretreated with BSP for 48 h were moved to 35°C or exposed to paraquat and their survival was recorded in order to determine the effect of BSP on stress resistance of C. elegans. As shown in [[Figure 3]a] and [[Figure 3]b], C. elegans pretreated with 50 μg/ml of BSP showed significantly increased lifespan after both thermal and oxidative stress, indicating that BSP improves the stress resistance of C. elegans.
|Figure 3: Bletilla striata polysaccharide (BSP) improves the stress resistance of Caenorhabditis elegans. (a) Survival curves of C. elegans pretreated with 50 ug/ml of BSP in 35°C. (b) Mean lifespans of C. elegans pretreated with or without BSP in 35°C. (c) Survival curves of C. elegans pretreated with 50 ug/ml of BSP in paraquat-containing medium. (d) Mean lifespans of C. elegans pretreated with BSP in paraquat-containing medium (*P < 0.05, **P < 0.01)|
Click here to view
The effect of Bletilla striata polysaccharide on bacterial growth and reproduction of Caenorhabditis elegans
Previous work showed that the inhibition of E. coli OP50, present in these assays as the food source for the nematodes, can lead to the extended lifespan of C. elegans. In order to investigate whether the anti aging of BSP is dependent on its anti microbial effect, we studied the effect of BSP on E. coli growth. As shown in [Figure 4], BSP has no effect on growth of E. coli OP50 at the doses effective to extend lifespan of C. elegans, indicating that the anti aging effect of BSP is not through inhibition of bacterial growth.
|Figure 4: The effect of Bletilla striata polysaccharide (BSP) on bacterial growth. Escherichia coli OP50 was seeded to medium with or without BSP and then transferred to rocking shaker at 37°C. OD595 values were measured for 10 h and growth curves of Escherichia coli OP50 in both mediums were prepared. Data are representative of three experiments|
Click here to view
The aging process of C. elegans can be delayed when its reproduction is reduced., To investigate whether the anti aging effect of BSP is due to reproduction reduction, we measured and compared the brood size of nematodes with or without BSP treatment. As shown in [Figure 5], there is no significant difference on brood size of C. elegans, indicating that the anti aging effect of BSP does not occur by altering the reproductive system of C. elegans.
|Figure 5: The effect of Bletilla striata polysaccharide (BSP) on reproduction of Caenorhabditis elegans.The reproduction of C. elegans was assessed by its brood size. worms were treated with BSP during their egg-laying period, and the endpoint was measured when worms developed into the adults|
Click here to view
Bletilla striata polysaccharide extends lifespan of Caenorhabditis elegans through insulin/insulin like growth factor signaling pathway
The aging process of C. elegans is regulated by several signaling pathways. Among all the pathways regulating C. elegans aging, the insulin/insulin like growth factor (IGF) signaling pathway might be the most well studied. Mutations of Daf 2 and age 1, key components of the insulin/IGF signaling pathway, have been found to extend the lifespan of C. elegans and transcription factor Daf 16 extends lifespan through regulation of longevity genes., In addition to its roles in lifespan extension, the insulin/IGF signaling pathway also regulates resistance to thermal stress, oxidative stress, and infections.
It is interesting to know whether BSP extends lifespan of C. elegans through insulin/IGF signaling pathway considering that BSP extends the lifespan of C. elegans and enhances the stress resistance of C. elegans. We first analyzed the expression change of daf 2, age 1, daf 16, and hcf 1, which are key components of the insulin/IGF signaling pathway, after BSP treatment through RT PCR. As shown in [[Figure 6]a], the expression of age 1 and hcf 1 were down regulated after BSP treatment. The expression of other genes, however, was not significantly changed. We next examined whether BSP can extend the lifespan of daf 16 mutant animals. As shown in [[Figure 6]b], the lifespan of daf 16 mutant animals was not extended after BSP treatment. These results indicate that the insulin/IGF signaling pathway is the target of BSP involved in the lifespan extension of nematodes.
|Figure 6: Bletilla striata polysaccharide (BSP) extends lifespan of Caenorhabditis elegans through insulin/insulin-like growth factor (IGF) signaling pathway. (a) Effects of BSP on expression patterns of key members in insulin/IGF signaling pathway. The nematodes were treated with 50 ug/ml of BSP for 48 h and then subjected to real-time-polymerase chain reaction. The expression levels of target genes were normalized to act-1 (**P < 0.01). (b) Survival curves of mutant nematodes daf-16 treated with 50 ug/ml of BSP|
Click here to view
| Discussion|| |
In the present study, we demonstrated that polysaccharide isolated from B. striata significantly extended the lifespan of C. elegans and it had best lifespan extending effect at the concentration of 50 μg/ml [Figure 1]. Moreover, worms treated with BSP showed greatly improved locomotion behavior during the aging process compared with control animals, especially in the latter stage of life [Figure 2]. These results showed that BSP has potential anti aging effects.
Worms pre treated with BSP exhibited increased survival rate compared to control animals under both thermal stress and oxidative stress conditions [Figure 3]. These results indicated that BSP has beneficial effects on C. elegans under stress conditions in addition to its lifespan extending effects under normal conditions.
In an effort to investigate the possible mechanisms for its anti-aging effect, we found that BSP has no effect on E. coli growth or brood size of C. elegans [Figure 4] and [Figure 5], indicating that the anti aging effect of BSP is neither mediated by effects on bacterial growth nor effects on the reproductive system of C. elegans.
The aging process of C. elegans is regulated by several signaling pathways such as the insulin/IGF signaling pathway, the target of rapamycin (TOR) signaling pathway, and the germline signaling pathway. In the insulin/IGF signaling pathway, the receptor DAF 2 activates a kinase cascade consisting of phosphatidiylinositol 3 kinase (PI3K/AGE 1), 3 phosphoinositide dependent kinase 1, and serine/threonine protein kinase (SGK 1). SGK 1 then inactivates FOXO transcription factor DAF 16 resulting in the blocking of expression of DAF 16 regulated genes.
We studied the mRNA levels of key genes from the insulin/IGF signaling pathway after BSP treatment and found the mRNA levels of age 1 and hcf 1 but not daf 16, were significantly reduced [[Figure 6]a]. We next studied the lifespan extending effect of BSP on daf 16 mutant animals and found that BSP could not extend the lifespan of daf 16 mutant animals [[Figure 6]b]. These results indicate that the anti aging effect of BSP is through its interaction with insulin/IGF signaling pathway. Because BSP does not change the mRNA level of daf 16, its lifespan extending effect is not through the direct control of daf 16 expression. However, our findings do not exclude the possibility that BSP may exert additional effects to extend the lifespan of C. elegans through other mechanisms such as by altering the nuclear translocation of DAF 16 protein.
| Conclusion|| |
Our results demonstrated that BSP has lifespan extending effects on nematode C. elegans. In addition, BSP was found to have the ability to improve the locomotion behavior and stress resistance of C. elegans. Moreover, our data revealed that the lifespan extending effect of BSP is dependent on insulin/IGF signaling pathway and is not via effects on either bacterial growth or reproduction of C. elegans.
Our current study provides the first evidence that BSP has anti aging properties. Future studies are warranted to examine the anti aging effects of BSP in mammals and to develop BSP as a functional food.
| Acknowledgments|| |
This work was supported by National Natural Science Foundation of China (31300707 and 31100224), Scientific Starting Fund from Changzhou University (ZMF11020011), National Science and Technology Support Program (2011BAD33B03).
| References|| |
Gutie´rrez RM. Orchids: A review of uses in traditional medicine, its phytochemistry and pharmacology. J Med Plants Res 2010;4:592 638.
Jiang F, Li W, Huang Y, Chen Y, Jin B, Chen N, et al
. Antioxidant, antityrosinase and antitumor activity comparison: The potential utilization of fibrous root part of Bletilla striata (Thunb.) Reichb.f. PLoS One 2013;8:e58004.
Wang C, Sun J, Luo Y, Xue W, Diao H, Dong L, et al
. A polysaccharide isolated from the medicinal herb Bletilla striata
induces endothelial cells proliferation and vascular endothelial growth factor expression in vitro. Biotechnol Lett 2006;28:539 43.
Diao H, Li X, Chen J, Luo Y, Chen X, Dong L, et al
. Bletilla striata
polysaccharide stimulates inducible nitric oxide synthase and proinflammatory cytokine expression in macrophages. J Biosci Bioeng 2008;105:85 9.
Luo Y, Diao H, Xia S, Dong L, Chen J, Zhang J. A physiologically active polysaccharide hydrogel promotes wound healing. J Biomed Mater Res A 2010;94:193 204.
Wu XG, Xin M, Chen H, Yang LN, Jiang HR. Novel mucoadhesive polysaccharide isolated from Bletilla striata
improves the intraocular penetration and efficacy of levofloxacin in the topical treatment of experimental bacterial keratitis. J Pharm Pharmacol 2010;62:1152 7.
Dong L, Xia S, Luo Y, Diao H, Zhang J, Chen J, et al
. Targeting delivery oligonucleotide into macrophages by cationic polysaccharide from Bletilla striata
successfully inhibited the expression of TNF alpha. J Control Release 2009;134:214 20.
Rui HY, Wu GR, Chen JY, Lu CM. Study on antioxidant activity of neutral polysaccharide from Bletilla striata. J Nanjing Norm Univ (Nat Sci Ed) 2003;26:94 9.
Fusco D, Colloca G, Lo Monaco MR, Cesari M. Effects of antioxidant supplementation on the aging process. Clin Interv Aging 2007;2:377 87.
Obrenovich ME, Li Y, Parvathaneni K, Yendluri BB, Palacios HH, Leszek J, et al
. Antioxidants in health, disease and aging. CNS Neurol Disord Drug Targets 2011;10:192 207.
Cho SC, Park MC, Keam B, Choi JM, Cho Y, Hyun S, et al
. DDS, 4,4’ diaminodiphenylsulfone, extends organismic lifespan. Proc Natl Acad Sci U S A 2010;107:19326 31.
Zhang W, Lv T, Li M, Wu Q, Yang L, Liu H, et al
. Beneficial effects of wheat gluten hydrolysate to extend lifespan and induce stress resistance in nematode Caenorhabditis elegans
. PLoS One 2013;8:e74553.
Wilkinson DS, Taylor RC, Dillin A. Analysis of aging in Caenorhabditis elegans
. Methods Cell Biol 2012;107:353 81.
Shen L, Hu Y, Cai T, Lin X, Wang D. Regulation of longevity by genes required for the functions of AIY interneuron in nematode Caenorhabditis elegans
. Mech Ageing Dev 2010;131:732 8.
Evason K, Huang C, Yamben I, Covey DF, Kornfeld K. Anticonvulsant medications extend worm life span. Science 2005;307:258 62.
Sangha JS, Sun X, Wally OS, Zhang K, Ji X, Wang Z, et al
. Liuwei Dihuang (LWDH), a traditional Chinese medicinal formula, protects against ß-amyloid toxicity in transgenic Caenorhabditis elegans
. PLoS One 2012;7:e43990.
Yang X, Zhang P, Wu J, Xiong S, Jin N, Huang Z. The neuroprotective and lifespan extension activities of Damnacanthus officinarum extracts in Caenorhabditis elegans
. J Ethnopharmacol 2012;141:41 7.
Rui Q, Lu Q, Wang DY. Administration of Bushenkangshuai Tang alleviates the UV irradiation and oxidative stress induced lifespan defects in nematode Caenorhabditis elegans
. Front Med China 2009;3:76 90.
Chen JY, Wu GR, Wang JA, Lu CM, Zhang WM, Jiang JH. Sulfation techniques of Bletilla striata
polysaccharide by orthogonal design. Chin Tradit Herb Drugs 2005;36:43 6.
Sulston JE, Brenner S. The DNA of Caenorhabditis elegans
. Genetics 1974;77:95 104.
Donkin S, Williams PL. Influence of developmental stage, salts and food presence on various end points using Caenorhabditis elegans
for aquatic toxicity testing. Environ Toxicol Chem 1995;14:2139 47.
Zhuang Z, Zhao Y, Wu Q, Li M, Liu H, Sun L, et al
. Adverse effects from clenbuterol and ractopamine on nematode Caenorhabditis elegans
and the underlying mechanism. PLoS One 2014;9:e85482.
Wilson MA, Shukitt Hale B, Kalt W, Ingram DK, Joseph JA, Wolkow CA. Blueberry polyphenols increase lifespan and thermotolerance in Caenorhabditis elegans
. Aging Cell 2006;5:59 68.
Hsin H, Kenyon C. Signals from the reproductive system regulate the lifespan of C. elegans
. Nature 1999;399:362 6.
Kim YS, Seo HW, Lee MH, Kim DK, Jeon H, Cha DS. Protocatechuic acid extends lifespan and increases stress resistance in Caenorhabditis elegans
. Arch Pharm Res 2014;37:245 52.
Lapierre LR, Hansen M. Lessons from C. elegans
: Signaling pathways for longevity. Trends Endocrinol Metab 2012;23:637 44.
Kaletsky R, Murphy CT. The role of insulin/IGF like signaling in C. elegans
longevity and aging. Dis Model Mech 2010;3:415 9.
Kenyon C. The first long lived mutants: Discovery of the insulin/IGF 1 pathway for ageing. Philos Trans R Soc Lond B Biol Sci 2011;366:9 16.
Evans EA, Kawli T, Tan MW. Pseudomonas aeruginosa suppresses host immunity by activating the DAF
2 insulin like signaling pathway in Caenorhabditis elegans
. PLoS Pathog 2008;4:e1000175.
McColl G, Rogers AN, Alavez S, Hubbard AE, Melov S, Link CD, et al
. Insulin like signaling determines survival during stress via posttranscriptional mechanisms in C. elegans
. Cell Metab 2010;12:260 72.
Lithgow GJ, White TM, Melov S, Johnson TE. Thermotolerance and extended life span conferred by single gene mutations and induced by thermal stress. Proc Natl Acad Sci U S A 1995;92:7540 4.
Larsen PL. Aging and resistance to oxidative damage in Caenorhabditis elegans
. Proc Natl Acad Sci U S A 1993;90:8905 9.
Kenyon CJ. The genetics of ageing. Nature 2010;464:504 12.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]
|This article has been cited by|
||Preparation, Characterization, and Antioxidative Activity of Bletilla striata Polysaccharide/Chitosan Microspheres for Oligomeric Proanthocyanidins
| ||Kang Liu,Zhanqin Feng,Lu Shan,Tingting Yang,Meng Qin,Jinbao Tang,Weifen Zhang |
| ||Drying Technology. 2017; |
|[Pubmed] | [DOI]|
||Current Perspective in the Discovery of Anti-aging Agents from Natural Products
| ||Ai-Jun Ding,Shan-Qing Zheng,Xiao-Bing Huang,Ti-Kun Xing,Gui-Sheng Wu,Hua-Ying Sun,Shu-Hua Qi,Huai-Rong Luo |
| ||Natural Products and Bioprospecting. 2017; |
|[Pubmed] | [DOI]|
||Bletilla striata: Medicinal uses, phytochemistry and pharmacological activities
| ||Xirui He,Xiaoxiao Wang,Jiacheng Fang,Zefeng Zhao,Linhong Huang,Hao Guo,Xiaohui Zheng |
| ||Journal of Ethnopharmacology. 2017; 195: 20 |
|[Pubmed] | [DOI]|
||Partial structural characterization, as well as immunomodulatory and anti-aging activities of CP2-c2-s2 polysaccharide from Cordyceps militaris
| ||Xiaojuan Liu,Yicheng Huang,Yunjiao Chen,Yong Cao |
| ||RSC Adv.. 2016; 6(106): 104094 |
|[Pubmed] | [DOI]|
||Optimization of infrared-assisted extraction of Bletilla striata polysaccharides based on response surface methodology and their antioxidant activities
| ||Yan Qu,Chunxue Li,Chen Zhang,Rui Zeng,Chaomei Fu |
| ||Carbohydrate Polymers. 2016; 148: 345 |
|[Pubmed] | [DOI]|
||Screening lifespan-extending drugs in Caenorhabditis elegans via label propagation on drug-protein networks
| ||Hui Liu,Mengmeng Guo,Ting Xue,Jihong Guan,Libo Luo,Ziheng Zhuang |
| ||BMC Systems Biology. 2016; 10(S4): 509 |
|[Pubmed] | [DOI]|
||A Living Model for Obesity and Aging Research:Caenorhabditis elegans
| ||Peiyi Shen,Yiren Yue,Yeonhwa Park |
| ||Critical Reviews in Food Science and Nutrition. 2016; : 00 |
|[Pubmed] | [DOI]|
||Preparation and evaluation of novel hydrogel based on polysaccharide isolated fromBletilla striata
| ||Xiuming Cui,Xingying Zhang,Ye Yang,Chengxiao Wang,Chaoyu Zhang,Gang Peng |
| ||Pharmaceutical Development and Technology. 2016; : 1 |
|[Pubmed] | [DOI]|