Assay method for quality control and stability studies of a new anti-diabetic and anti-dyslipidemic flavone (S002-853)*
Arshi Naqvi1, Anuja Pandey1, Varsha Gupta1, Richa Malasoni1, Akansha Srivastava1, Rishi Ranjan Pandey1, M Satyanarayana2, Ram Pratap2, Anil Kumar Dwivedi1
1 Division of Pharmaceutics, Central Drug Research Institute, Lucknow, Uttar Pradesh, India
2 Medicinal and Process Chemistry Division, Central Drug Research Institute, Lucknow, Uttar Pradesh, India
|Date of Submission||24-Jun-2014|
|Date of Acceptance||18-Sep-2014|
|Date of Web Publication||27-May-2015|
Dr. Anil Kumar Dwivedi
Division of Pharmaceutics, Central Drug Research Institute, BS 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow - 226 021, Uttar Pradesh
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Flavonoid-rich extract of the plant is long known for its anti-diabetic activities in traditional medicine. S002-853, a new flavone derivative synthesized by Central Drug Research Institute (CDRI) has been used for the present study. Objectives: The present study aimed at development of an assay method for quality control (QC) and stability studies of a new anti-diabetic and anti-dyslipidemic agent CDRI compound S002-853. Materials and Methods: A validated high-performance liquid chromatography analysis method for S002-853 was developed for in process QC and stability studies. The separation was achieved on a RP-C18 (25 cm × 0.4 cm, 5 μm, Phenomenex) at 240 nm with flow rate of 1.0 ml/min. This method was applied successfully in establishing forced degradation and drug-excipient testing protocols as per International Conference on Harmonization guidelines. Results: The result of estimation and stress testing studies indicated a high degree of selectivity of this method. S002-853 was most stable at pH 7 and under photolytic conditions. The temperature degradation pattern of S002-853 was found to follow the zero order degradation. Conclusion: The method described is easy and simple hence can be easily reproduced. This method can be very useful for bulk manufacture QC, and drug development process.
Keywords: Anti-diabetic, anti-dyslipidemic, flavone derivative, high performance liquid chromatography, in process quality control
|How to cite this article:|
Naqvi A, Pandey A, Gupta V, Malasoni R, Srivastava A, Pandey RR, Satyanarayana M, Pratap R, Dwivedi AK. Assay method for quality control and stability studies of a new anti-diabetic and anti-dyslipidemic flavone (S002-853)*. Phcog Mag 2015;11, Suppl S1:53-9
|How to cite this URL:|
Naqvi A, Pandey A, Gupta V, Malasoni R, Srivastava A, Pandey RR, Satyanarayana M, Pratap R, Dwivedi AK. Assay method for quality control and stability studies of a new anti-diabetic and anti-dyslipidemic flavone (S002-853)*. Phcog Mag [serial online] 2015 [cited 2021 Apr 23];11, Suppl S1:53-9. Available from: http://www.phcog.com/text.asp?2015/11/42/53/157689
FNx01C.D.R.I. Communication No. 8847
| Introduction|| |
Diabetes mellitus is one of the most common endocrine metabolic disorders and had become a global epidemic with significant impact on society and economy. There are 382 million people living with diabetes and may reach 471 million by 2035. The rapid increase in type-2 diabetes mellitus (T2DM) among people aged between 30 and 39 years, and in children including adolescents, is of particular concern.  The prevalence increases with age and varies widely between different populations and ethnic groups. T2DM (non-insulin-dependent) accounts for approximately 90% of patients with diabetes mellitus, with type-1 (insulin-dependent) accounting for the remainder.  One of the main contributing factors to this burden is the changed sedentary life style, which promotes obesity as a result incidence of diabetes.  Patients with T2DM have increased cardiovascular mortality due to recurrent thrombotic events. , Several epidemiology studies have revealed that patients with T2DM are at increased risk for developing memory impairment, dementia and Alzheimer's disease.  Characteristic factors of this heterogeneous disorder include insulin resistance, obesity, hypertension and a common form of dyslipidemia, low high-density lipoprotein cholesterol, in insulin sensitivity and impaired insulin secretion by pancreatic β cells.  Although the primary cause of type-2 diabetes remains unclear, the inability of β cells to compensate for reduced insulin sensitivity in peripheral tissues eventually leads to β-cell failure and deterioration in glucose homeostasis, ultimately leading to overt T2DM. T2DM is a complex disease and both genetic, and environmental factors appear to contribute to its development. ,,
Medicinal plants play an important role in the management of diabetes mellitus especially in developing countries where resources are meager. Flavonoids and chalcones are among the most ubiquitous groups of polyphenolic compounds in foods of plant origin. As integral constituents of the diet, they may exert a wide range of beneficial effects on human health. Flavonoid-rich extract of the plant is long known for its anti-diabetic activities in traditional medicines. ,, Flavonoids and chalcones produce such biological effects through their free radical scavenging anti-oxidant activities and metal ion chelating abilities. , Within the secondary metabolite of the flavonoid class, flavones define one of the largest subgroups. They express various biological activities such as anti-bacterial, anti-cancer, anti-tumor, anti-protozoal, anti-oxidant, anti-convulsant. ,
In the quest to develop compound with efficacy, low toxicity, and more ever affordable for treatment we utilized flavones for the synthesis of hybrid molecule as anti-diabetic and anti-dyslipidemic agents by substitution with thermogenic as well as insulin sensitizing pharmacophores. Central Drug Research Institute (CDRI), Lucknow while shouldering the responsibility of this project, accepted the challenge to develop new anti-diabetic and anti-dyslipidemic as well as to test their activity. A few compounds have had been launched in the market, and several are still in the preclinical phase from this institute. S002-853 [Figure 1], C 36 H 37 NO 6 ] is such derivative, demonstrated to exhibit promising anti-diabetic and anti-dyslipidemic activity. ,,, Compound hence was taken up for further studies pertaining to the development of pharmaceutical dosage form.
| Materials and Methods|| |
Standard S002-853 was prepared in the Medicinal and Process Chemistry Division. Milli-Q pure water was obtained from a Millipore Elix water purification system (Millipore India Pvt. Ltd. [New Delhi, India]). High-performance liquid chromatography (HPLC) grade methanol and acetonitrile (ACN) were purchased from Merck, Ltd. (Mumbai, India). Sodium hydroxide, hydrochloric acid, hydrogen peroxide, acetic acid, sodium bicarbonate, sodium metabisulphite, sodium chloride, sodium nitrate, potassium chloride of certified grades were purchased from SD Fine Chem Ltd, Mumbai, India. Other reagents used were of analytical grade. Excipients namely hydroxypropyl methyl cellulose (HPMC), methyl cellulose, ethyl cellulose, lactose, polyvinyl pyrrolidone (PVP), micro crystalline cellulose, magnesium stearate was purchased from SD Fine Chem Ltd, Mumbai, India and Sigma Aldrich.
Apparatus and chromatographic conditions
The HPLC workstation used was from Shimadzu, Japan; equipped with SCL-10A VP system controller, LC-10AT VP twin pump, SPD-10A VP UV-VIS detector, photo diode array detector (SPD M10A), Rheodyne injector with 20 μl injection loop. The separation was achieved on a reverse phase analytical column (RP) C-18 (25 cm × 0.4 cm, 5 μm, Phenomenex). The mobile phase consisted of a mixture of ACN: 0.05% glacial acetic acid in water (70:30) system. Both the solvents were filtered and degassed before use. Chromatography was performed at 27°C ± 3°C at a flow rate of 1.0 ml/min. The monitoring wavelength for the column effluent was taken at 240 nm. The data acquisition and processing were done using Shimadzu HPLC software Class-VP (V 5.03). Humidity chambers and photostability chambers (Thermolab, Mumbai, India) used were equipped with UV and visual light tubes.
Preparation of calibration standards and quality control samples
Stock standard solution of compound S002-853 was prepared by weighing ~ 10 mg of the compound and making the volume to 10 ml with ACN. Working standard solutions were prepared by serial dilution in ACN in the range of 1-50 μg/ml. The quality control (QC) samples were prepared at three different concentration levels (1 μg/mL [low QC], 6.25 μg/mL [medium QC] and 25.0 μg/mL [high QC]) for S002-853.
Preparation of sample solution
The sample of the compound S002-853 was prepared by weighing 5 mg of different batches and dissolving in 25 ml of ACN, and then 1 ml of that was further diluted in 10 ml. Similarly, the samples from various studies were weighed and diluted.
The method was validated with respect to parameters including selectivity, limit of quantitation (LOQ), limit of detection (LOD), linearity, precision, accuracy, ruggedness, robustness and recovery according to International Conference on Harmonization (ICH) guidelines.
Specificity and selectivity
The chromatographic interferences were assessed by comparing chromatograms of blank ACN with that of samples spiked with S002-853 in ACN. Specificity was established by determination of peak purity index of the drug peak using a photodiode array (PDA) detector.
The linearity of standard solutions was evaluated by analyzing a set of standards ranging from 1 to 50 μg/mL. Plots of peak area (response) against analyte concentration were used. The slope, intercept and correlation coefficient of each calibration curve were determined using linear regression (LINREG) analysis. In the regression equation y = mx + c, where m corresponds to the slope of line, x is the concentration of standard solution in μg, y is the peak area and c is the intercept of the straight line on y axis.
Limits of detection and quantitation
The LOD and LOQ were measured according to the ICH guidelines (ICH 2003). LOD and LOQ were estimated with the signal to noise ratio of 3 and 10, respectively.
Intra-day and inter-day accuracy and precision
In order to evaluate the intra- and inter-day precision and accuracy of the assay, QC samples at low, medium and high concentrations were prepared as described above. The intra-day precision of the assay was assessed by calculating the coefficients of variation (CV) for the analysis of QC samples in three replicates and inter-day precision was determined by the analysis of three replicates QC samples on three different days. The concentrations of S002-853 were quantitated using the LINREG line of the calibration standards. The percent deviation of the calculated concentrations of the standard from the actual concentrations (% deviation from the actual concentration, [% DFA]) was used to calculate the intraday and inter-day accuracy. The % relative standard deviation (RSD) was used to calculate inter-day precision.
The variable factors used to determine the robustness of the analytical method were percentage of ACN, flow rate of mobile phase and age of the column (new and old column).
The ruggedness of the method was tested by using two HPLC systems of Class-VP, Shimadzu Corporation, Kyoto, Japan consisted of binary pump having UV detector and HPLC system described above. The parameters were checked within replicates in intraday and inter-day accuracy and precision by variations.
Known amount of S002-853 was added to the mixed contents of the samples, and the quantity of S002-853 was determined by interpolation on the corresponding calibration graphs. The recovery was calculated by using the following equation: Recovery (%) = (Amount detected) ×100/(Amount spiked) %.
The purity of the compound was checked, and its identity was established by HPLC and nuclear magnetic resonance/fast atom bombardment-mass, respectively.
The solubility of S002-853 was checked in various solvents such as methanol, ethanol, chloroform, acetone, ACN, ethyl acetate, and dichloromethane. This was done by taking 1 ml of each solvent and adding an excess amount of the compound to it. The amount dissolved in the solvent was determined by HPLC. All the experiments were performed in triplicate.
Partition coefficient (log P)
Log P for S002-853 was calculated by shaking the compound with the octanol/buffer (0.05M phosphate buffer, pH = 7.4) and octanol/water layer mixture for about 4 h, then centrifugation for 20 min at 4000 rpm. Then, after the layers were separated, each was analyzed by HPLC after proper dilution and concentration were calculated.
The dissociation constant determination (pKa) value of any compound can be determined using different methods such as the potentiometry, spectrophotometry and capillary electrophoresis. Spectrophotometric determination of dissociation constant (pKa) for S002-853 using Albert equation was carried out. In this method, absorbance of S002-853 in different pH (ranging from acidic pH 2, 3, 4, 5, 6 and basic pH 8, 9, 10, 11 and 12 solutions) using spectrophotometric method was observed.
Where, A I and A M represent the absorbance of the basic and the acidic form, respectively, of S002-853, and A denotes the absorbance at the given pH and wavelength.
Effect of pH
Acid-base degradation studies were carried out to obtain the pH for maximum stability. This information is required for the preparation of the final formulation. Effect of pH on this compound was checked by taking 1 ml of stock solution (each) of S002-853 in 10 ml volumetric flasks and the volumes were made up with buffers of pH 2-10. Samples were withdrawn at different time intervals, diluted appropriately, and 20 μl were injected on to the HPLC column to analyze as described above. The reaction rate constants were calculated by LINREG program.
Effect of temperature
For conducting accelerated temperature stability studies, the samples of S002-853 (50 mg) were kept at 37°C, 50°C and 60°C for 2 months in different ovens. The samples so stored were examined for caking, liquefaction, discoloration, odor and gas formation. The sample (10 mg) was withdrawn from vials at every 10 days up to 2 months and dissolved in ACN and diluted suitably for estimation of products by HPLC method.
Effect of temperature and moisture as per international conference on harmonization guidelines
In the presence of moisture, many drug substances hydrolyze, react with other excipients or oxidize. These reactions can be accelerated by exposing the solid drug to different relative humidity conditions. Three vials of S002-853 were kept at 4°C (in refrigerator), 30°C ± 2°C with 65% ±5% RH and 40°C ± 2°C with 75% ±5% RH (in Thermolab humidity cum photo stability chambers). The stored samples were examined for discoloration and odor or gas formation. The sample (2 mg) was withdrawn from each vial after regular intervals (0, 3, 6, 9, 12, 18 and 24 months). They were dissolved, diluted suitably in ACN and analyzed by HPLC.
The photolytic stability of S002-853 was performed by exposing S002-853 to light from UV lamp in a photolytic chamber (Thermolab) at 1.2 million/h Luxes. The drug (20 mg) was placed in Petri dish More Detailses and exposed to UV light for a period of 57 h. The samples were withdrawn after 5, 24, and 57 h and analyzed by HPLC. The drug stored under the same conditions but protected from light was used as control for comparison.
Forced degradation studies
S002-853 was subjected to various forced degradation conditions as per ICH guidelines to effect partial degradation preferably in 20-30% range. S002-853 was refluxed (40°C) with three different reagents, that is, alkali (0.1 N NaOH), acid (0.1 N HCl) and peroxide (3% H 2 O 2 ) for 1 h, followed by neutralization with acetic acid, sodium bicarbonate and sodium metabisulfite for alkali, acid and peroxide, respectively, and final volume was made up to 10 ml with methanol. This was further diluted five times with ACN and injected (20 μl) in triplicate on HPLC. The % remaining concentration of S002-853 was calculated with the help of the reference standard.
Drug excipient studies
Interaction between the active constituent and excipients can alter stability and bioavailability of drugs, thereby, affecting its safety and/or efficacy. A number of experimental techniques (i.e. differential scanning calorimetry, Fourier transform infrared spectroscopy, X-ray powder diffraction, scanning electron microscopy, HPLC etc.) have been used to investigate the interaction between drug and excipients. , Equimolar mixtures (100 mg each) of S002-853 and the chosen excipients were maintained at 40°C ± 2°C for a period of 7 days in glass vials. A control sample containing only the extract was also kept under similar conditions. After 7 days, all samples were withdrawn, and 5 ml methanol-water (1:1) solution was added to each vial. These samples were then individually vortexed to ensure mixing and then filtered. The subsequent filtrate achieved of concentration 20 mg/ml was further diluted to achieve the final test samples of 1 mg/ml each. These were analyzed by HPLC for estimation of S002-853 content and to observe any changes in the fingerprint pattern or additional peaks.
| Results|| |
Final separation was achieved in an isocratic mobile phase consisting of a mixture of ACN: 0.05% glacial acetic acid (70:30) system at a flow rate of 1 mL/min. This method was capable in resolving S002-853 at a retention time of about 7.9 min with quality peak shape [Figure 2]. The PDA studies indicated that the method was sufficiently specific. The calibration curves (n = 3) showed a linear relationship between peak area and concentration over the range of 1-50 μg/mL with the correlation coefficient (r2 ) of 0.998. Based on the signal-to-noise ratio of 3 and 10, the LOD came out to be 0.284 μg/ml. However, the LOQ was 2.84 μg/ml. The recoveries of the analyte samples were in the range of 98-101% and the % CV and % DFA were not more than 3% [Table 1].
The compound S002-853 was found 98.85% pure by HPLC. The solubility data are given in [Table 2]. The partition coefficient of S002-853 in octanol and water is 2.77, and that in octanol and phosphate buffer pH 7.4 is 3.00. Spectrophotometric determinations of the pKa and pKb values of S002-853 were found to be 4.2 and 9.8, respectively.
It was found that the drug is most stable at pH 7 [Table 3]. The temperature degradation rate constant at 25°C was calculated to be K = 0.17457/week, t 1/2 = 5.5 years, t 10 = 57.28 weeks and Ea. =59.73 kJ/mole [Table 4]. At controlled humidity environments and upon exposure to UV lamp (negligible decomposition) drug is quite stable. In forced degradation studies, stress conditions utilizing alkali medium afforded maximum degradation, followed by acidic and consecutively peroxide. The drug was not found compatible with PVP [Figure 3].
|Figure 3: Differential scanning calorimetry of S002-853 with various excipients|
Click here to view
| Discussion|| |
The purpose of the study was to develop a validated HPLC method for in process QC and stability studies of CDRI compound S002-853. The chromatographic conditions were optimized to obtain chromatograms with better peak shape within a short time. Out of the many permutations and combinations employed the most suitable and optimized solvent system was chosen. Initially, we tried the separation experiments with columns like C-18 endcapped (250 mm, 4 mm, 5 μm, Merck) or C 8 (250 mm, 4 mm, 5 μm, Merck) or CN (250 mm, 4 mm, 5 μm, Merck). Several solvent systems comprising of different percentage of methanol-water or ACN-water were used, but the resolution among the peaks of the main compound not found satisfactory. The best separation was achieved on a RP C-18 (25 cm × 0.4 cm, 5 μm, Phenomenex).
The PDA studies indicated that the method was sufficiently specific. The purity angle value was less than the threshold angle, indicating that the drug peak was pure by nature. The result of estimation and stress testing studies indicated a high degree of selectivity of this method. No interfering peaks were observed at the retention time of the analyte. The method provided adequate sensitivity for the determination of S002-853 in bulk drug substance and dosage forms. Precision of the assay was investigated with respect to both repeatability and reproducibility. The percentage RSD of assay S002-853 during assay method precision and in intermediate precision study were within acceptable limits (ICH 2003) confirming good precision of the method. The accuracy was also found to be within acceptable limits at all calibration points. The analytical method was found to be rugged with instrumental and environmental variation and also sufficiently robust within the range of tested conditions.
Physico-chemical parameters and stability studies
The solubility data indicate that the compound S002-853 is lipophilic in nature, and it is slightly soluble in water.
The samples of pH studies were collected at different days and analyzed by HPLC. The % remaining concentration was calculated from the calibration curves. The temperature degradation pattern of S002-853 was found to follow the zero order degradation, which indicated that the degradation of the compound is concentration independent. The rate constant (K), half-life (t 1/2 ), shelf-life (t 10 ) and energy of activation (E a ) at 25°C were calculated by LINREG. The % concentration remaining of S002-853 was found to be 23.87% in alkaline medium (0.1 N NaOH), 50.43% in acidic medium (0.1 N HCl), and 65.81% in 3% H 2 O 2 . In the drug excipients interaction, it was found that micro crystalline cellulose; lactose and magnesium stearate has no effect on S002-853. HPMC, EC and MC retard the release of the drug from the matrix [Figure 3].
| Conclusion|| |
The developed and validated HPLC method is reproducible and can be routinely used for QC of S002-853 in bulk manufacture, with ongoing stability studies for drug development process. Analytical studies were attempted on CDRI compound S002-853 to generate data beneficial for formulation development, further studies and Investigational New Drug application on the same. It provides base line separation of the compound of interest S002-853. The bulk samples of compound S002-853 from the Medicinal and Process Chemistry were checked by the above method. The results of stress testing undertaken according to the ICH guide lines reveal that the method is also selective and stability-indicating for S002-853. The solubility data indicate that the compound S002-853 is lipophilic in nature, and it is slightly soluble in water. The drug candidate is most sensitive towards alkaline condition as compared to acidic and oxidative conditions. The reported method can be very useful for further studies on bulk manufacture QC and in drug development process.
| Acknowledgments|| |
Authors are thankful to Council of Scientific and Industrial Research, University Grant Commission, Ministry of Health and Family Welfare, Government of India, New Delhi, for providing financial assistance.
| References|| |
World Diabetes Foundation (EDF). Diabetes Facts. IDF Diabetes Atlas. 6 th
ed. Brussels, Belgium: International Diabetes Federation; 2013. Available from: http://www.idf.org/diabetes
atlas. [Last accessed on 2014 May 20].
Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047-53.
Gillies CL, Abrams KR, Lambert PC, Cooper NJ, Sutton AJ, Hsu RT, et al
. Pharmacological and lifestyle interventions to prevent or delay type 2 diabetes in people with impaired glucose tolerance: Systematic review and meta-analysis. BMJ 2007;334:299.
Krempf M, Parhofer KG, Steg PG, Bhatt DL, Ohman EM, Röther J, et al.
Cardiovascular event rates in diabetic and nondiabetic individuals with and without established atherothrombosis (from the REduction of Atherothrombosis for Continued Health [REACH] Registry). Am J Cardiol 2010;105:667-71.
Viswanathan GN, Marshall SM, Balasubramaniam K, Badimon JJ, Zaman AG. Differences in thrombus structure and kinetics in patients with type 2 diabetes mellitus after non ST elevation acute coronary syndrome. Thromb Res 2014;133:880-5.
Qiu CX, Winblad B, Fratiglioni L. Risk factors for dementia and Alzheimer′ s disease-findings from a community-based cohort study in Stockholm, Sweden. Zhonghua Liu Xing Bing Xue Za Zhi 2005;26:882-7.
Henry RR. Glucose control and insulin resistance in non-insulin-dependent diabetes mellitus. Ann Intern Med 1996;124:97-103.
Zhai Y, Zhao J, You H, Pang C, Yin L, Guo T, et al.
Association of the rs11196218 polymorphism in TCF7L2 with type 2 diabetes mellitus in Asian population. Meta Gene 2014;2:332-41.
Mandrup-Poulsen T. Diabetes. BMJ 1998;316:1221-5.
Pickup JC, Crook MA. Is type II diabetes mellitus a disease of the innate immune system? Diabetologia 1998;41:1241-8.
Sharma B, Viswanath G, Salunke R, Royet P. Effects of flavonoid-rich extract from seeds of Eugenia jambolana
0 (L.) on carbohydrate and lipid metabolism in diabetic mice. Food Chem 2008;110:697-705.
Takashi S, Hiroyuki O, Yu M, Mitsuru K. Oxazolidinedione derivatives, their production and use, U.S. Patent, 5932601; 1999.
Liao Z, Chen X, Wu M. Antidiabetic effect of flavones from Cirsium japonicum
DC in diabetic rats. Arch Pharm Res 2010;33:353-62.
Cotelle N, Bernier JL, Hénichart JP, Catteau JP, Gaydou E, Wallet JC. Scavenger and antioxidant properties of ten synthetic flavones. Free Radic Biol Med 1992;13:211-9.
Vaya J, Belinky PA, Aviram M. Antioxidant constituents from licorice roots: Isolation, structure elucidation and antioxidative capacity toward LDL oxidation. Free Radic Biol Med 1997;23:302-13.
Kowalski K, Koceva-Chyła A, Szczupak L, Hikisz P, Bernasińska J, Rajnisz A, et
al. Ferrocenylvinyl-flavones: Synthesis, structure, anticancer and antibacterial activity studies. J Organomet Chem 2013;741:153-61.
Sharma A, Bharadwaj S, Maan AS, Jain A, Kharya MD. Screening methods of antioxidant activity: An overview. Pharmacogn Rev 2007;1:232-8.
Sandhar HK, Kumar B, Prasher S, Tiwari P, Salhan M, Sharma P. A review of phytochemistry and pharmacology of flavonoids. Int Pharm Sci 2011;1:25-41.
Verma AK, Singh H, Satyanarayana M, Srivastava SP, Tiwari P, Singh AB, et al.
Flavone-based novel antidiabetic and antidyslipidemic agents. J Med Chem 2012;55:4551-67.
Pratap R, Singh H, Verma AK, Singh AB, Tiwari P, Srivastava M, et al. S-(+)-7-[3-N-Substituted amino-2-hydroxypropoxy] flavone. European Patent EP 2057137 B1; 2013.
Singh H, Pratap R. A convenient one-pot synthesis of 7-hydroxyisoflavones from resorcinol with substituted phenylacetic acids. Tetrahedron Lett 2006;47:8161-3.
Shukla P, Singh AB, Srivastava AK, Pratap R. Chalcone based aryloxypropanolamines as potential antihyperglycemic agents. Bioorg Med Chem Lett 2007;17:799-802.
Bharate SS, Bharate SB, Bajaj AN. Interactions and incompatibilities of pharmaceutical excipients with active pharmaceutical ingredients: A comprehensive review. J Excip Food Chem 2010;1:3-26.
Bozdag-Pehlivan S, Subasi B, Vural I, Unlü N, Capan Y. Evaluation of drug-excipient interaction in the formulation of celecoxib tablets. Acta Pol Pharm 2011;68:423-33.
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3], [Table 4]