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Cytotoxic and apoptotic effects of Ebenus boissieri Barbey on human lung cancer cell line A549
Esra Arslan Aydemir1, Ece Simsek2, Nilüfer Imir3, Ramazan Süleyman Göktürk1, Erdem Yesilada4, Kayahan Fiskin1
1 Department of Biology, Faculty of Science, Akdeniz University, 07058, Antalya, Turkey
2 Department of Nutrition and Dietetics, Antalya School of Health, Akdeniz University, 07058, Antalya, Turkey
3 Department of Biology Education, Faculty of Education, and Institute of Life Sciences, Akdeniz University, 07058, Antalya, Turkey
4 Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, Atasehir 34755, Istanbul, Turkey
Correspondence Address:
Dr. Esra Arslan Aydemir
Department of Biology, Faculty of Science, Akdeniz University, 07058, Antalya
Turkey
 Source of Support: This work was supported by The Scientific
Research Project Coordination Unit of Akdeniz University (Project no:
2012.06.0115.032) and Akdeniz University, Science Faculty, Department of
Biology, Conflict of Interest: None  | Check |
DOI: 10.4103/0973-1296.157679
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Background: Fabaceae family members are known to possess preventive and therapeutic potentials against various types of cancers. Objective: The aim of this study was to investigate the cytotoxic and apoptotic effects of hydroalcoholic extracts from the aerial parts and roots of an endemic Ebenus species; Ebenus boissieri Barbey in human lung cancer cell line. Materials and Methods: After treatment with hydroalcoholic extracts cytotoxic activities of both extracts were measured by 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay, whereas caspase-3 activity, tumor necrosis factor-alpha (TNF-α) and interferon gamma (IFN-γ) release were measured by enzyme linked immunosorbent assay. Results: According to in vitro assay results, the increase in all caspases activity suggested that extracts induce cells to undergo apoptosis. Especially, induction in caspase-3 activity was the most remarkable result of this study. Both aerial part and root extracts induced apoptosis by increasing caspase-3 activity, TNF-α and IFN-γ release. When compared to their relative controls, the concentrations of both TNF-α and IFN-γ in extract-treated groups were significantly and dose dependently exalted. Conclusion: Taken together, our results indicate that the hydroalcoholic extracts of E. boissieri can be considered as a source of new anti-apoptotic and therefore anti-carcinogenic agent. |
