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Evaluating Nothapodytes nimmoniana population from three localities of Western Ghats using camptothecin as phytochemical marker and selection of elites using a new-content range chart method
Gireesh Ankad1, Vinayak Upadhya1, Sandeep R Pai2, Mansingraj S Nimbalkar3, Harsha V Hegde4, Rajesh K Joshi5, Sanjiva D Kholkute6
1 Ethnomedicine Division, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India
2 Plant Biotechnology and Tissue Culture Division, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India
3 Department of Botany, Shivaji University, Kolhapur, Maharashtra State, India
4 Ethnomedicine Division; Plant Biotechnology and Tissue Culture Division, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India
5 Phytochemistry Division, Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India
6 Ethnomedicine Division; Plant Biotechnology and Tissue Culture Division; Phytochemistry Division, Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India
Correspondence Address:
Sandeep R Pai
Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belgaum - 590 010, Karnataka State
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0973-1296.149712
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Background: Nothapodytes nimmoniana (Grah.) Mabb. is a high valued medicinal plant endemic to Western Ghats of India, distributed in fragmented populations. The plant is valued for potent anticancer drug camptothecin (CPT). Objective: The study compares and expounds variation in CPT content from leaves and stems of N. nimmoniana obtained from three populations of Western Ghats, India. The study also describes a method for categorizing these populations using content range chart (CRC) method for percent yield of CPT. Materials and Methods: A total of 60 samples were investigated including ten each of leaves and stems from three localities. Micro-extraction method was implemented to extract CPT. reversed phase ultra-performance liquid chromatography photo diode array technique was used to quantify CPT. Results: Leaf samples of an individual collected from Joida, yielded lowest CPT content (0.002 ± 0.000 g/100 g), whereas a stem sample from Amgaon, yielded highest CPT content (0.123 ± 0.006 g/100 g). The findings suggest great variation in individuals producing and accumulating CPT. Using this data along with earlier published work, five categories of CPT yielding plants were made viz. I: Very low: <0.020, II: Low: 0.021-0.039, III: Moderate: 0.040-0.059, IV: High: 0.060-0.079 and V: Very high: >0.080. Based on CPT content in leaves, majority of individuals were under very low category (I st ) and on the other hand stem samples were in 'II' category. Besides, very few individuals were observed in category 'V'. Conclusion: The study expounds use of CRC method for identifying elite population and suggests the need for its conservation. |
