Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2015  |  Volume : 11  |  Issue : 41  |  Page : 14-23

Flavonoid profiles of three Bupleurum species and in vitrohepatoprotective activity of Bupleurum flavum Forsk.

1 Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria
2 Department of Pharmacology, Pharmacotherapy and Toxicology, Laboratory of Drug Metabolism and Drug Toxicity, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria

Correspondence Address:
Reneta Gevrenova
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, 2, Dunav Street, 1000 Sofia
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1296.149680

Rights and Permissions

Background: Bupleurum L. (Aspiaceae) species are used as herbal remedy in Chinese traditional medicine. Objective: The aim was to investigate the flavonoids in three annual European Bupleurum species, including B. baldense, B. affine and B. flavum, and to test their antioxidant and possible hepatoprotective effects. Materials and Methods: Flavonoids from the methanol-aqueous extracts were quantified by solid-phase extraction-high-performance liquid chromatography. Bupleurum extracts (1-220 mg/ml) were tested for their antioxidant activity in DPPH and ABTS assays, as well as on isolated liver rat microsomes. In vitro hepatoprotective activity of B. flavum flavonoid (BFF) mixture and rutin, and narcissin, isolated from the same mixture, were evaluated on carbon tetrachloride (CCl 4 ) and tert-butyl hydroperoxide (t-BuOOH) toxicity models in isolated rat hepatocytes. Results: Narcissin was the dominant flavonol glycoside in B. flavum being present at 24.21 ± 0.19 mg/g, whilst the highest content of rutin (28.63 ± 1.57 mg/g) was found in B. baldense. B. flavum possessed the strongest DPPH (IC 50 22.12 μg/ml) and ABTS (IC 50 118.15 μg/ml) activity. At a concentration 1 mg/ml of BFF (rutin 197.58 mg/g, narcissin 75.74 mg/g), a stronger antioxidant effect in microsomes was evidenced in comparison with silymarin, rutin and narcissin. The hepatoprotective effect of BFF significantly reduced the elevated levels of lactate dehydrogenase and malondialdehyde, and ameliorated glutathione, being most active in t-BuOOH-induced injury model when compared with CCl 4 toxicity (P < 0.001). Conclusion: In BFF, synergism of rutin and narcissin could be responsible for stronger protection against mitochondrial induced oxidative stress.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded110    
    Comments [Add]    
    Cited by others 4    

Recommend this journal