Leucas aspera inhibits the Dalton's ascitic lymphoma in Swiss albino mice: A preliminary study exploring possible mechanism of action

Bibin Baby Augustine; Suvakanta Dash; Mangala Lahkar; Usha Sarma; Pavan Kumar Samudrala; Jaya Mary Thomas

doi:10.4103/0973-1296.131022

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ORIGINAL ARTICLE

Year : 2014 | Volume : 10 | Issue : 38 | Page : 118-124

Leucas aspera inhibits the Dalton's ascitic lymphoma in Swiss albino mice: A preliminary study exploring possible mechanism of action

Bibin Baby Augustine¹, Suvakanta Dash², Mangala Lahkar¹, Usha Sarma³, Pavan Kumar Samudrala¹, Jaya Mary Thomas⁴
¹ Laboratory of Tumor Pharmacology, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Guwahati, Assam, India
² Department of Pharmaceutics, Girijananda Institute of Pharmaceutical Sciences, Azara, Guwahati, Assam, India
³ Department of Pathology, Gauhati Medical College, Guwahati, Assam, India
⁴ Department of Pharmacology, College of Pharmaceutical Sciences, Medical College, Trivandrum, India

Correspondence Address:
Bibin Baby Augustine
Laboratory of Tumor Pharmacology, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Guwahati - 781 032, Assam
India

Source of Support: NIPER-Guwahati, Department of Pharmaceuticals, Ministry of Chemicals and Fertilisers, Government of India, Conflict of Interest: None

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DOI: 10.4103/0973-1296.131022

Background: North East India is a rich source of medicinal plants and a number of plant extracts are used by tribal peoples living in this area for various disorders. L.aspera is such a plant, traditionally used as an antitumor agent. Aim: In the present study, aerial parts of L.aspera were investigated for antitumor activity in Dalton's lymphoma (DAL) bearing mice. The ability of plant extract in free radical scavenging, neoangiogenesis inhibition and macrophage stimulation were also checked. Materials and Methods: Based on the preliminary in vitro cytotoxicity studies ethyl acetate fraction of L.aspera (EALA) was selected for the detailed study. DAL ascites tumor model was performed to check the antitumor activity of EALA (200 and 400mg/kg of body weight). Hematological and histopathological parameters were estimated. Antioxidant levels, neoangiogenesis and peritoneal macrophage count were also determined. Results: In vitro MTT and Trypan blue assay results showed the cytotoxic effect of EALA in DAL cells lines. EALA treatment resulted in significant decrease in ascites tumor volume and viable cell count. Hematological and liver antioxidant parameters were normalised by EALA treatment. It was also found that EALA treatment inhibits neovascularisation and produce macrophage stimulation in treated mice. Conclusion: The results showed that EALA is a promising anticancer agent and its activity is comparable to the standard drug 5-Flouro uracil (5-FU).

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