Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2014  |  Volume : 10  |  Issue : 37  |  Page : 65-71

In vitro and in silico studies on the anticancer and apoptosis-inducing activities of the sterols identified from the soft coral, subergorgia reticulata

Inter University Centre for Development of Marine Biotechnology, Cochin University of Science and Technology, Cochin, India

Correspondence Address:
S Muraleedharan Nair
Inter University Centre for Development of Marine Biotechnology, Cochin University of Science and Technology, Cochin - 682 016
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1296.127345

Rights and Permissions

Background: Gorgonians and other octocorals are known to possess a huge array of secondary metabolites in which sterols are the major group of secondary metabolites apart from sesquiterpenes and diterpenes, and the bioactive metabolites could show marked biomedical potential for future drug discovery. Objective : This study was intended for the isolation and identification of sterols from the octocoral Subergorgia reticulata and to evaluate the anticancer and apoptosis-inducing activities of the identified sterols through in vitro and in silico approach. Materials and Methods : The organism was collected from Lakshadweep Island. The isolated sterols were identified using Gas chromatography-mass spectrometry (GC-MS). The structure was confirmed by using comparison of their spectra those in National Institute of Standard Technology (NIST) library. The apoptosis inducing effect of identified sterols were determined by PASS online prediction. In vitro cytotoxity studies were carried out using Dalton's lymphoma ascites cells (DLA) and the cell viability was determined by trypan blue exclusion method. Results : Six sterols were identified from the soft coral S. reticulata. They are Cholesta-5,22-diene-3ol (3β), Ergosta-5-22-dien-3ol (3β,22E 24S), Cholesterol, 26,26-Dimethyl-5,24(28)-ergostadien-3β-ol. β-sitosterol, and Fucosterol. In silico predictions showed that the identified sterols exhibited remarkable apoptosis agonist activity. The probability of apoptosis agonist activity were found maximum for 26,26-Dimethyl-5,24 (28)-S. reticulata sterol fractions isolated were found to be having anticancer activity. Conclusions : These findings suggest that S. reticulata contained biologically active sterol compounds that may be useful in the treatment of cancer.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded134    
    Comments [Add]    
    Cited by others 10    

Recommend this journal