| ORIGINAL ARTICLE |
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| Year : 2014 | Volume
: 10
| Issue : 37 | Page : 57-64 |
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Optimization of quercitrin and total flavonoids extraction from Herba Polygoni Capitati by response surface methodology
Fengwei Ma1, Yang Zhao1, Xiaojian Gong1, Yu Xie2, Xin Zhou1
1 The Research Center for Quality Control of Nature Medicine; Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou province, Guizhou Nomal University, Guiyang, Guizhou, China
2 Guizhou Warmen Pharmaceutical Co., Ltd., Guiyang, Guizhou, China
Correspondence Address:
Xin Zhou
The Research Center for Quality Control of Nature Medicine, Guizhou Normal University, Guiyang, Guizhou, 550 001
China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0973-1296.127343
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Objective: To optimize the conditions for extraction of quercitrin and total flavonoids (TF) from Herba Polygoni Capitati (Touhualiao in Chinese) by using response surface methodology (RSM). Materials and Methods: A central composite design (CCD) was adopted to investigate the effects of three independent variables including solvent composition (%), solvent-material ratio (ml/g) and extraction time (min) on the responses, quercitrin and TF yields. Results: The optimized conditions of the extraction are as follows: Ethanol concentration, 65.63%; solvent-material ratio, 10.55:1 (ml/g); extraction time, 54.33 min. The established mathematical model described the factors of experimental parameters well and provided a statistically accurate prediction of the optimum yields of quercitrin and TF. Conclusion: The experimental values agreed with those predicted by the established mathematical model, thus indicating the suitability of the model employed and the success of RSM in optimizing the extraction conditions. |
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