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Year : 2014  |  Volume : 10  |  Issue : 37  |  Page : 22-29

Simultaneous determination of five major compounds in the traditional medicine Pyeongwee-San by high performance liquid chromatography-diode array detection and liquid chromatography-mass spectrometry/mass spectrometry

1 Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Korea
2 Department of Medical Biomaterials Engineering, College of Biomedical Science; Research Institute of Biotechnology, Kangwon National University, Chuncheon 200-701, Korea

Correspondence Address:
Choong Je Ma
Department of Biomaterials Engineering, Division of Biotechnology and Bioengineering, Kangwon National University, Hyoja-2 Dong, Chuncheon 200-701
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1296.127335

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Background: Pyeongwee-San (PWS) has been widely used for treating acute gastritis, chronic, and gastritis. Objective: In this paper, simultaneous determination of five compounds (naringin, hesperidin, glycyrrhizin, atractylenolide III, and magnolol) from traditional medicine PWS using the high performance liquid chromatography (HPLC) was established for quality control. Materials and Methods: Optimum separations were obtained with a SHISEIDO C18 reverse-phase column by gradient elution with 0.1% Trifluoroacetic acid (TFA) water-acetonitrile as the mobile phase. The flow rate was 1 mL/min and detection wavelength was set at 205 nm and 250 nm. Validation of the analytical method was evaluated by linearity, precision, and accuracy test. Results: The calibration curves were linear over the established range with R 2 > 0.9978. The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.09 to 0.43 and 0.27 to 1.29 μg/mL. The method exhibited intra-day and inter-day precision range between 0.01-1.86% and 0.04-0.35% respectively. The recoveries of five compounds in PWS were in the range between 93.18-106.40%, and 0.20-1.51%. The application of this method was identified through the successful analysis of five compounds in 12 batches of PWS. In addition, identification of five compounds was confirmed by a liquid chromatography method and mass spectrometry. Conclusion: The HPLC method was could be accomplished to the quality control and stable experiment for the preparations consisted of five major compounds.

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