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ORIGINAL ARTICLE
Year : 2013  |  Volume : 9  |  Issue : 34  |  Page : 96-102

Development and application of a method for determination of nucleosides and nucleobases in Mactra veneriformis


Marine Medicine Research and Development Center, College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing, China

Correspondence Address:
Hao Wu
College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing
China
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Source of Support: The financial grant of this work has been supported by the R&D Special Fund for Public Welfare Industry from State Ocean Administration P.R. China (No. 200709005) and National Natural Science Foundation of China (No. 0900293)., Conflict of Interest: None


DOI: 10.4103/0973-1296.111241

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Background: Mactra veneriformis, a typical marine bivalve mollusk, delicious sea food while low cost, is ubiquitous and abundant in Chinese coastal areas, especially in the coastal shoals of Jiangsu province. To our knowledge, previously reported analytical methods can not meet a set of quality control. Objective: For the simultaneous determination of eight components (uridine, inosine, guanosine, thymidine, adenosine, xanthine, thymine and hypoxanthine) in M. veneriformis, a high performance liquid chromatography with UV detector method was established. Materials and Methods: To develop the method, a reverse phase column, BioBasic-C 18 (5 μm, 4.6 mm × 250 mm) was used. The mobile phase consisted of methanol and water using a gradient elution. The UV wavelength was set at 245 nm. The analysis conditions including extraction methods, extraction solvents, and HPLC parameters were optimized systematically for achieving good separation. Linearity, accuracy, repeatability and detection limit was revealed and showed good performance. Results: The optimized HPLC method was successfully applied for the qualititation of 5 nucleosides namely, uridine, inosine, guanosine, thymidine, adenosine and 3 nucleobases namely, xanthine, thymine, hypoxanthine in M. veneriformis. Conclusion: A method with less time-consuming, more sensitive, and more precise was developed for the quantitative determination of nucleosides and nucleobases in M. veneriformis extractions. The established method might apply as an alternative approach for the quality assessment of M. veneriformis.


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