Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2013  |  Volume : 9  |  Issue : 34  |  Page : 96-102

Development and application of a method for determination of nucleosides and nucleobases in Mactra veneriformis

Marine Medicine Research and Development Center, College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing, China

Correspondence Address:
Hao Wu
College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing
Login to access the Email id

Source of Support: The financial grant of this work has been supported by the R&D Special Fund for Public Welfare Industry from State Ocean Administration P.R. China (No. 200709005) and National Natural Science Foundation of China (No. 0900293)., Conflict of Interest: None

DOI: 10.4103/0973-1296.111241

Rights and Permissions

Background: Mactra veneriformis, a typical marine bivalve mollusk, delicious sea food while low cost, is ubiquitous and abundant in Chinese coastal areas, especially in the coastal shoals of Jiangsu province. To our knowledge, previously reported analytical methods can not meet a set of quality control. Objective: For the simultaneous determination of eight components (uridine, inosine, guanosine, thymidine, adenosine, xanthine, thymine and hypoxanthine) in M. veneriformis, a high performance liquid chromatography with UV detector method was established. Materials and Methods: To develop the method, a reverse phase column, BioBasic-C 18 (5 μm, 4.6 mm × 250 mm) was used. The mobile phase consisted of methanol and water using a gradient elution. The UV wavelength was set at 245 nm. The analysis conditions including extraction methods, extraction solvents, and HPLC parameters were optimized systematically for achieving good separation. Linearity, accuracy, repeatability and detection limit was revealed and showed good performance. Results: The optimized HPLC method was successfully applied for the qualititation of 5 nucleosides namely, uridine, inosine, guanosine, thymidine, adenosine and 3 nucleobases namely, xanthine, thymine, hypoxanthine in M. veneriformis. Conclusion: A method with less time-consuming, more sensitive, and more precise was developed for the quantitative determination of nucleosides and nucleobases in M. veneriformis extractions. The established method might apply as an alternative approach for the quality assessment of M. veneriformis.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded98    
    Comments [Add]    
    Cited by others 3    

Recommend this journal