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Year : 2012  |  Volume : 8  |  Issue : 29  |  Page : 22-28

Isolation and characterization of a bactericidal withanolide from Physalis virginiana

1 Department of Biology and Microbiology, South Dakota State University, Brookings, SD, USA
2 Department of Chemistry and Biochemistry, South Dakota State University, Brookings, SD, USA

Correspondence Address:
Kathleen A Gibson
Department of Biology and Microbiology, Box 2140D, South Dakota State University, Brookings, SD 57007
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1296.93307

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Background: Physalis virginiana (Virginia Groundcherry) is a member of the family Solenaceae. Several species of the Physalis genus have been used traditionally by American Indians as medicinal treatments. Materials and Methods: This study investigated the antibacterial activity of chemicals extracted from P. virginiana through antibacterial disc and cytotoxicity assays. Isolation and purification of an antimicrobial compound was achieved through flash chromatography and preparative HPLC. Finally, identification of chemical structure was determined from 1 H and 13 C NMR and MS. Results: Disc assays showed that crude ethanol extracts were effective antibacterial agents against one gram-negative and seven gram-positive bacterial strains. Cytotoxicity assays indicated that it is less toxic than gentamicin controls. Isolation of the active component showed it to be a relatively polar compound. 1 H and 13 C NMR chemical shifts together with HRMS indicated a similar structure to withanolides previously identified from Physalis angulata. HRMS analysis showed a molecular mass of 472.2857 which corresponds to a molecular formula C 28 H 40 O 6 . Conclusion: An antibacterial withanolide was isolated from P. virginiana using flash chromatography and HPLC separations. The chemical structure was determined by NMR and MS to be the withanolide physagulin V.

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