Standardization of spray-dried powder of Piper betle hot water extract
Liyanage Dona Ashanthi Menuka Arawwawala1, Horadugoda Gamage Sujatha Pushpakanthi Hewageegana2, Lakshmi Sriyani Rajapaksha Arambewela1, Hettiarachchige Sami Ariyawansa2
1 Industrial Technology Institute, Bauddhaloka Mawatha, Colombo 7, Sri Lanka
2 Department of Nidana Chikitsa, Institute of Indigenous Medicine, University of Colombo, Rajagiriya, Sri Lanka
|Date of Submission||20-May-2010|
|Date of Decision||27-Nov-2010|
|Date of Web Publication||9-May-2011|
Liyanage Dona Ashanthi Menuka Arawwawala
Industrial Technology Institute, Bauddhaloka Mawatha, Colombo 7
Source of Support: None, Conflict of Interest: None
| Abstract|| |
The leaves of Piper betle Linn. (Family: Piperaceae) possess several bioactivities and are used in the Traditional Medical systems of Sri Lanka. The present investigation was carried out to standardize the spray-dried powder of P. betle by (a) determination of physicochemical parameters, presence or absence of heavy metals, and microbial contamination; (b) screening for phytochemicals; and (c) development of High Pressure Liquid Chromatography (HPLC) fingerprint and densitogram. The percentages of moisture content, total ash, acid insoluble ash, water-soluble ash, and ethanol extractable matter of spray-dried powder of P. betle were 2.2-2.5, 6.8-7.0, 0.003-0.005, 4.1-4.3, and 15.8-16.2, respectively. The concentrations of all the tested heavy metals were below the WHO acceptable limits and bacterial species, such as Escherichia coli, Salmonella spp, Staphylococcus aureus, and Pseudomonas aeroginosa were not present in the P. betle spray-dried powder. Phenolic compounds, tannins, flavonoids steroids, and alkaloids were found to be present in the spray-dried powder of P. betle and HPLC fingerprint and densitogram clearly demonstrated the proportional differences of these chemical constituents. In conclusion, the results obtained from this study can be used to standardize the spray-dried powder of P. betle.
Keywords: Piper betle , physicochemical parameters, phytochemicals
|How to cite this article:|
Arawwawala LA, Hewageegana HS, Arambewela LR, Ariyawansa HS. Standardization of spray-dried powder of Piper betle hot water extract. Phcog Mag 2011;7:157-60
|How to cite this URL:|
Arawwawala LA, Hewageegana HS, Arambewela LR, Ariyawansa HS. Standardization of spray-dried powder of Piper betle hot water extract. Phcog Mag [serial online] 2011 [cited 2021 May 11];7:157-60. Available from: http://www.phcog.com/text.asp?2011/7/26/157/80678
| Introduction|| |
Plant-based drugs have been in use against various diseases since time immemorial. The primitive man used herbs as therapeutic agents, which they were able to procure easily.  Piper betle Linn. (Family: Piperaceae) is a perennial dioecious, semi-woody climber and cultivated in Sri Lanka, India, Malaysia, Indonesia, Philippine Islands, and East Africa. Stems are strongly swollen at the nodes, papillose when young, soon entirely glabrous. Leaves alternate, simple, and yellowish green to bright green in color. Leaves of fertile branches with a petiole 1-2 cm long, 1.2-1.8 mm thick when dry and glabrous at maturity. , More than 12 P. betle cultivars are reported in Sri Lanka  and except cultivar called Malabulath, which is not used for chewing, other cultivars constitute the "commercial betel" of Sri Lanka. According to Kumaratunga,  chemical constituents and their relative proportions in essential oil of "commercial betel" of Sri Lanka are different from those of other countries. In Sri Lanka, research work has been carried out to evaluate some selected bioactivities, such as antimotility effects on washed human spermatozoa,  antiaphrodisiac activity,  antifertility effects of male rats,  antimicrobial,  antidiabetic,  antinociceptive  and antioxidant  activities using hot water extract of P. betle leaves.
In modern herbal drug industry, there is a trend to manufacture tablets and capsules from spray-dried powders of bioactive plant extracts. Examples include Amla spray-dried powder, Neem spray-dried powder, Arjuna spray-dried powder, Turmeric spray-dried powder, Noni spray-dried powder, Aleo vera spray-dried powder and so on. Therefore, an attempt was made to prepare a spray-dried powder from P. betle hot water extract and standardize the spray-dried powder by (a) determining the physicochemical parameters, (b) screening the phytochemical constituents, and (c) developing the High Pressure Liquid Chromatography (HPLC) fingerprints and densitograms.
| Materials and Methods|| |
P. betle leaves were purchased from the main vegetable markets in the Western province of Sri Lanka. The leaves were identified and authenticated by the Curator of National Herbarium, Royal Botanical Gardens, Peradeniya, Sri Lanka. A voucher specimen (PS 01) was deposited in the Industrial Technology Institute, Colombo 7, Sri Lanka.
Preparation of spray-dried powder from hot water extract of Piper betle0
P. betle leaves were air dried for 3-5 days in the shade, cut into small pieces and 20 kg were boiled with 100 L of distilled water for 4 h. The extract was filtered and the filtrate was concentrated under vacuum until the total solid content exceeded more than 25%. The concentrated extract was further subjected to a spray dryer (Kestner Patent Spray Dryer, made in UK; inlet: 175°C and outlet: 100°C) to obtain the powder form the extract.
Determination of physicochemical parameters of Piper betle spray-dried powder
Physicochemical parameters such as moisture content, total ash content, acid insoluble and water soluble ash contents and ethanol extractable matter were determined for P. betle spray-dried powder according to the methods described in guidelines of WHO. 
Determination of moisture content
The powdered material (1 g) was placed in a moisture dish and dried to a constant weight in an oven at 100 °C-105 °C. The loss of weight in mg/g of air-dried material was calculated.
Determination of total ash content
The powdered material (2 g) was accurately weighed and placed in a crucible. The material was ignited to a constant weight by gradually increasing the heat to 500 °C-600 °C until it was white. The residual ash was allowed to cool in a desicator. The content of total ash in mg/g of air dried material was calculated.
Determination of acid insoluble ash content
Hydrochloride acid (2 N; 25 mL) was added to the crucible containing the total ash, covered with a watch glass and boiled gently for 5 min. The watch glass was rinsed with 5 mL of hot water and the rinsed contents were added to the crucible. The acid insoluble matter was collected on an ashless filter paper and washed with hot water until the filtrate was neutral. The filter paper containing acid insoluble matter was transferred to the original crucible, dried and ignited to a constant weight. The residue was allowed to cool in a desicator and weighed. The content of the acid insoluble ash in mg/g of air dried material was calculated.
Determination of water soluble ash content
Water (25 mL) was added to the crucible containing the total ash, covered with a watch glass and boiled gently for 5 min. The watch glass was rinsed with 5 mL of hot water and added to the crucible. The water insoluble matter was collected on an ashless filter paper and washed with hot water. The filter paper containing the water insoluble matter was transferred to the original crucible, dried on a hot plate and ignited to a constant weight. The water soluble ash content was calculated.
Determination of ethanol extractable matter
Accurately weighed powdered material (4 g) was placed in a glass stoppered conical flask. Ethanol (95%; 100 mL) was added to the flask and it was weighed to obtain the total weight including the flask. Then, the flask was shaken well and kept for 1 h. A reflux condenser was attached to the flask and boiled gently for 1 h, and then it was cooled and weighed. The weight was readjusted to the original total weight by adding required amount of 95% ethanol. The contents were filtered rapidly. After that, 25 mL of the filtrate was evaporated to dryness on a water bath. Then the dish was dried at 105 °C for 6 h, cooled in a desiccator, and weighed. The content of extractable matter in mg/g air dried material was calculated.
Determination of heavy metals in the spray-dried powder
Presence or absence of heavy metals (Hg, Pb, Cd, and As) were determined according to SLS  and AOAC  standards.
Determination of microbial contamination in the spray-dried powder
Presence or absence of Escherichia More Details coli, Salmonella More Details spp, Staphylococcus aureus and Pseudomonas aeroginosa were determined according to the standards of SLS. ,,
Preliminary phytochemical screening of Piper betle spray-dried powder
The qualitative chemical tests were performed for the P. betle spray-dried powder according to the methods described by Farnsworth  with some modifications.
Determination of presence/absence of phenolic compounds
Two to three drops of 1% FeCl 3 solution was added into 2 mL portions (1%) of the spray-dried powder. Phenolic compounds produce a deep violet color with ferric ions.
Determination of presence/absence of tannins
The spray-dried powder was diluted with water and added to diluted FeCl 3 solution. Tannins give a blackish blue or green blackish color in the presence of FeCl 3.
Determination of presence/absence of flavonoids
The spray-dried powder was dissolved in methanol (50%, 1-2 mL) by heating. Then metal magnesium and 5-6 drops of conc. HCl were added. The solution turns red when flavonoids are present.
Determination of presence/absence of steroid glycosides
The spray dried powder was dissolved in equal volumes of acetic anhydride and CHCl 3 . The mixture was transferred to a dry test tube and conc. H 2 SO 4 acid was added at the bottom of the tube. Formation of a reddish brown or violet-brown ring at the interface of the two liquids indicates the presence of steroids.
Determination of presence/absence of alkaloids
The alkaloids were extracted by refluxing the sample with sufficient amount of water for about 2 h. The extract was concentrated, adjusted to a high pH (pH = 9.2) with NH 4 OH and was extracted with CHCl 3 (three times). Then the content was concentrated and 2 drops were spotted separately on a Thin Layer Chromatography plate. After the spots were dried, Dragendorff's reagent was sprayed onto them. Alkaloids give an orange color with Dragendorff's reagent.
Development of HPLC fingerprint
P. betle spray-dried powder (10 mg) was dissolved in 10 mL of distilled water and purified using Sep-Pak C 18 cartridge.
Injection volume : 20 μ L
Apparatus : Waters 501 HPLC pump SPD 10AV UV-vis detector (Shimadzu, Japan)
Column : Hypersil® ODS (C 18 )
Solvent system : Acetonitrile:Water (1:1)
Flow rate : 1 mL/min
Detection : 254 nm
Development of densitogram
P. betle spray-dried powder (10 mg) was dissolved in 10 mL of distilled water and 5 μL was spotted on Silica Gel 254
Absorbent : Silica Gel 254
Solvent system : Butanol:Methanol:Water (1:1:0.2)
Scanning : Densitometer (CS-9301PC, Shimadzu, Japan) at 254 nm.
| Results|| |
Results are listed in [Table 1].
|Table 1: Physicochemical parameters of spray-dried powder of Piper betle hot water extract |
Click here to view
Phytochemical screening studies
Phytochemical screening studies revealed the presence of phenolic compounds, tannins, flavonoids, steroids and alkaloids in the spray-dried powder of P. betle hot water extract.
HPLC fingerprint and densitogram
As shown in [Figure 1], spray-dried powder of P. betle hot water extract was standardized by using HPLC fingerprint and densitogram.
|Figure 1: (a) HPLC fingerprint; and (b) densitogram of spray-dried powder of Piper betle hot water extract|
Click here to view
| Discussion|| |
The residue remaining after incineration of plant material is the ash content or ash value, which simply represents inorganic salts, naturally occurring in crude drug or adhering to it or deliberately added to it, as a form of adulteration. The ash value was determined by 3 different methods, which measured total ash, acid insoluble ash, and water soluble ash. The total ash is used to measure the total amount of material remaining after ignition. This includes both "physiologic ash," which is derived from the plant tissue itself, and "nonphysiologic ash," which is the residue of the extraneous matter adhering to the plant surface. Acid insoluble ash measures the amount of silica or acid insoluble matter present. Water soluble ash is the water soluble portion of the total ash. These ash values are important quantitative standards. 
Extractive values are representative of the presence of the polar or nonpolar compounds in a plant material. The water soluble extractive of ginger is expected to be in the range of the 10% with respect to air dried material, lowering of this extractive value indicates the addition of exhausted material with the original drug. Moisture is an inevitable component of crude drugs, which must be eliminated as far as practicable. Insufficient drying favors spoilage by molds and bacteria and enzymatic destruction of active principles. ,
The presence or absence of 4 heavy metals, namely, As, Hg, Cd, and Pb, was analyzed in the P. betle spray-dried powder and results are shown in [Table 1]. The concentrations of all the tested heavy metals were below the WHO acceptable limits. As shown in [Table 1], bacterial species, such as Escherichia coli, Salmonella spp, Staphylococcus aureus, and Pseudomonas aeroginosa were not found in the P. betle spray-dried powder. Therefore, this is a good indicator of the hygiene of the preparation.
Standardization of herbal products/drugs is more challenging than synthetic drugs. Herbal extracts contain number of constituents of complex chemical nature and are inconsistent in composition.  In most of the cases the biological activity is not exclusively dependent on the so-called active constituents, but is due to synergistic effect of all the chemical constituents of the plant. Even though biologically inert, many constituents affect the pharmacokinetics and stability of the active constituents.  Phytochemical screening revealed the presence of phenolic compounds, tannins, flavonoids, steroids, and alkaloids in the P. betle spray-dried powder and standardized by using a HPLC fingerprint and a densitogram.
In conclusion, the results obtained from physicochemical parameters, phytochemical screening studies and development of HPLC fingerprint and densitogram can be used to standardize P. betle spray-dried powder.
| References|| |
|1.||Bhatti GR, Qureshi R, Shah M. Ethnobotany of Calotropis procera with especial reference to the people of Nara Desert. Scientific Sindh. Ann J Res 1988;5:13-22. |
|2.||Jayaweera DM. Medicinal Plants Used in Ceylon. Vol. 5. Colombo: National Science Council of Sri Lanka; 1982. p. 201. |
|3.||Dassanayake MD, Fosberg FR. A Revised Hand Book to the flora of Ceylon. New Delhi: Amreind; 1987. p. 287-8. |
|4.||Ratnasoma HA, Senevirathna JM. Betel cultivation. Sri Lanka: Department of Agriculture; 1985. p. 3-5. |
|5.||Kumaratunga KG. Gas chromatographical and antimicrobial studies on Alpinia calcarata and Piper betle from Sri Lanka. M. Phil Thesis, Sri Lanka: University of Kelaniya; 2003. |
|6.||Ratnasooriya WD, Jayawardena KG, Premakumara GAS. Antimotility effects of Piper betle (L) leaf extract on washed human spermatozoa. J Nat Sci Council Sri Lanka 1990;18:53-60. |
|7.||Ratnasooriya WD, Premakumara GAS. Piper betle leaves impairs masculine sexual behavior of rats. Med Sci Res 1996;24:303-6. |
|8.||Ratnasooriya WD, Premakumara GAS. Piper betle leaves reversibly inhibits fertility of male rats. Vidyodaya J Sci 1997;7:15-21. |
|9.||Arambewela LSR, Arawwawala LDAM, Ratnasooriya WD. Antidiabetic activities of aqueous and ethanolic extracts of Piper betle leaves in rats. J Ethnopharmacol 2005;102:239-45. |
|10.||Arambewela LSR, Arawwawala LDAM, Ratnasooriya WD. Antinociceptive activity of aqueous and ethanolic extracts of Piper betle leaves. Pharmaceut Biol 2005;43:766-72. |
|11.||Arambewela LSR, Arawwawala LDAM, Rajapaksa R. Piper betle: A potential natural antioxidant. Int J Food Sci Tech 2006;41:10-4. |
|12.||Quality control methods for medicinal plant materials. Geneva: World Health Organization; 1998. |
|13.||Sri Lanka Standard (SLS) 242: 1973, Bureau of Ceylon Standards. |
|14.||Association of Analytical Communities (AOAC) 999.10, 999.11:2000. |
|15.||Sri Lanka Standard (SLS) 516, Part 3:1982, Bureau of Ceylon Standards. |
|16.||Sri Lanka Standard (SLS) 516, Part 5:1992, Bureau of Ceylon Standards. |
|17.||Sri Lanka Standard (SLS) 516, Part 6:1992, Bureau of Ceylon Standards. |
|18.||Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci 1996;55:225-76. |
|19.||Singh MP, Sharma CS. Pharmacognostical evaluation of Terminalia chebula fruits on different market samples. Int J ChemTech Res 2010;2:57-61. |
|20.||Mukherjee PK. Quality Control of Herbal Drugs. Business Horizons, New Delhi, India, 2002. |
|21.||Jadhav RB, Patil CR, Bhpoe S, Murumkar CV. Herbal drug standardization and quality assurance of raw materials: a rational approach. Nat Prod Radat 2003;2:134-40. |
|22.||Handa SS. 4 th International Congress of Ethnopharmacology, precongress workshop on medicinal plants. Lucknow, India.1994. |