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Year : 2011  |  Volume : 7  |  Issue : 25  |  Page : 53-59  

Chemical composition and antimicrobial activities of the essential oils from three ecotypes of Zataria multiflora

1 Center of Basic Researches in Infectious Diseases; Department of Medical Mycology and Parasitology, School of Medicine, Shiraz University of Medical Sciences, Post code 71348-45794, Shiraz, Iran
2 Department of Horticultural Sciences, Faculty of Agriculture, Shiraz University, Shiraz, Iran
3 Center of Basic Researches in Infectious Diseases, School of Medicine, Shiraz University of Medical Sciences, Post code 71348-45794, Shiraz, Iran
4 Department of Medical Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Post code 71348-45794, Shiraz, Iran

Date of Submission09-Sep-2010
Date of Decision08-Dec-2010
Date of Web Publication20-Jan-2011

Correspondence Address:
M J Saharkhiz
Department of Horticultural Sciences, Faculty of Agriculture, Shiraz University, Shiraz
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Source of Support: The study was financially supported by the Shiraz University of Medical Sciences (grant number 88-01-01-1397), Conflict of Interest: None

DOI: 10.4103/0973-1296.75902

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Background: Zataria multiflora Boiss. is a traditional and popular spice in Iran. The effects of 3 ecotypes (ECTPs) of Z. multiflora essential oils (EOs) against most common causes of food-borne and nosocomial infections were evaluated. Materials and Methods: The antimicrobial activities of the EOs were examined by broth microdilution method as recommended by the Clinical and Laboratory Standards Institute (CLSI). The chemical compositions of the EOs from 3 ECTPs of Z. multiflora have been analyzed by gas chromatography-mass spectrometry. Results: Analysis of the EOs indicated that 3 chemotypes were present in Z. multiflora, including carvacrol, thymol-carvacrol, and linalool, whereas previous studies have only found carvacrol and thymol. Inhibition studies showed that the tested EOs entirely inhibited the growth of yeasts at concentrations of less than 1 μL/mL. Moreover, the oils exhibited significant bacteriostatic and bactericidal activities against Gram-positive and Gram-negative bacteria at concentrations ranging from 0.12 to 8 μL/mL. Conclusion: These results suggest that the EOs from Z. multiflora should be investigated further for possible use in antimicrobial products and food preservatives.

Keywords: Antimicrobial activity, chemotype, carvacrol, essential oil, linalool, thymol, Zataria multiflora

How to cite this article:
Zomorodian K, Saharkhiz M J, Rahimi M J, Bandegi A, Shekarkhar G, Bandegani A, Pakshir K, Bazargani A. Chemical composition and antimicrobial activities of the essential oils from three ecotypes of Zataria multiflora. Phcog Mag 2011;7:53-9

How to cite this URL:
Zomorodian K, Saharkhiz M J, Rahimi M J, Bandegi A, Shekarkhar G, Bandegani A, Pakshir K, Bazargani A. Chemical composition and antimicrobial activities of the essential oils from three ecotypes of Zataria multiflora. Phcog Mag [serial online] 2011 [cited 2022 Dec 4];7:53-9. Available from: http://www.phcog.com/text.asp?2011/7/25/53/75902

   Introduction Top

Zataria multiflora Boiss. with the common Persian name "Avishan-e Shirazi" is a thyme-like essential oil (EO)-bearing plant that belongs to the Lamiaceae family and grows extensively wild in the central and southern parts of Iran, Pakistan, and Afghanistan. [1] The dry aerial parts of the plant have been used for their flavor and preservative properties in the food products industry. [2] In Iran, Z. multiflora is mainly used in traditional folk remedies for its antiseptic, analgesic, and carminative (antiflatulence and intestine-soothing) properties. [1] It also has been reported that the EOs and extracts of Z. multiflora can stimulate innate immunity [3] and have antibacterial and antifungal activities. [1],[4],[5] In addition, Z. multiflora EOs have been shown to cause inhibitory effects against radial fungal growth and aflatoxin production by Aspergillus flavus in cheese. [2] Moreover, the oil and extracts of Z. multiflora successfully inhibited the growth of bacteria associated with gastrointestinal infections, including Staphylococcus aureus, [6] enterohemorrhagic  Escherichia More Details coli, [7]  Salmonella More Details Typhi and Paratyphi, [8] and Shigella flexneri and Bacillus cereus. [8],[9] In the past 2 decades, the emergence of resistance to various antibiotics has accelerated dramatically. Methicillin-resistant S. aureus (MRSA), vancomycin-resistant Enterococcus (VRE) species, third-generation cephalosporin-resistant (TGCsR) Escherichia coli, imipenem and quinolone-resistant Pseudomonas aeruginosa, antibacterial-resistant Salmonella and Shigella species, as well as azole-resistant Candida species are the top resistant pathogens responsible for food-borne or nosocomial infections. [10],[11] To overcome antibiotic resistance, there is a great tendency toward using natural products and phytochemicals in the medicine and food industries. EOs, especially with known antibacterial effects, have the potential to be used in the food industry as a preservative, for spoilage prevention, and to increase the shelf life of products. Therefore, determining the antimicrobial properties of EOs might help to overcome microorganism resistance to antibiotics.

To the best of our knowledge, only a few published reports are available regarding the antimicrobial effects of the Z. multiflora EOs, especially against the above-mentioned resistant microorganisms. In the present study, the chemical constituents of 3 ecotypes (ECTPs) of Z. multiflora were studied and their components were compared with each other and to previously reported data. In addition, the antimicrobial effects of these ECTPs were evaluated against standard strains and clinical isolates of nosocomial infections as well as some food-borne agents.

   Materials and Methods Top

Collection of plant material

Three ECTPs of Z. multiflora were used to extract the EOs. The plant ECTPs from which the EOs were extracted were collected from 3 different ecologic areas. The aerial parts of ECTP A, B, and C, including flowers were obtained from wild plants in Lamerd, Darab, and Zarghan regions in Fars province, Iran, respectively. Lamerd and Darab are about 855 m above the mean sea level with warm-dry climate, whereas Zarghan is about 1602 m and has a semi-arid climate.

The plant species were identified and authenticated by Dr. A.R. Khosravi, a plant taxonomist, at Shiraz University, Herbarium, Shiraz, Iran. Voucher specimens (No. 24984, 24985, and 24986) were deposited in the herbarium.

EO preparation

At full flowering stage, the aerial parts of the ECTPs were hydrodistillated for 2.5 h, using an all-glass Clevenger-type apparatus, according to the method outlined by the British Pharmacopoeia. [12] The sample oils were dried over anhydrous sodium sulfate and stored in sealed vials at 4°C before gas chromatography and gas chromatography-mass spectrometry (GC-MS) analysis.

EO analysis by gas chromatography-mass spectrometry

The EOs were analyzed by GC-MS. The analysis was carried out on a Thermoquest-Finnigan Trace GC-MS instrument equipped with a DB-5 fused silica column (60 m ×0.25 mm i.d., film thickness 0.25 mm). The oven temperature was programmed to increase from 60°C to 250°C at a rate of 4°C/min and finally held for 10 min; transfer line temperature was 250°C. Helium was used as the carrier gas at a flow rate of 1.1 mL/min with a split ratio equal to 1/50. The quadrupole mass spectrometer was scanned over the 35-465 amu with an ionizing voltage of 70 eV and an ionization current of 150 mA.

GC- flame ionization detector (FID) analysis of the oil was conducted using a Thermoquest-Finnigan instrument equipped with a DB-5 fused silica column (60 m × 0.25 mm i.d., film thickness 0.25 mm). Nitrogen was used as the carrier gas at the constant flow of 1.1 mL/min; the split ratio was the same as for GC-MS. The oven temperature was raised from 60°C to 250°C at a rate of 4°C/min and held for 10 min. The injector and detector (FID) temperatures were kept at 250°C and 280°C, respectively. Semi-quantitative data were obtained from FID area percentages without the use of correction factors.

Identification of EO components

Retention indices (RI) were calculated by using retention times of n-alkanes (C6-C24) that were injected after the oil at the same temperature and conditions. The compounds were identified by comparing their RI with those reported in the literature, and their mass spectrum was compared with those reported in Wiley Library. [13]

Determination of antimicrobial activities


The antifungal activities of the EO against 5 American Type Culture Collection (ATCC) strains of fungi, including Candida albicans (ATCC 10261), C. tropicalis (ATCC 750), C. krusei (ATCC 6258), C. glabrata (ATCC 90030), and C. parapsilosis (ATCC 4344) were determined. In addition, the antimicrobial activities of the EO against 40 clinical isolates of yeasts identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were also examined. [14],[15] The antibacterial activities of the EO against standard species of S. aureus (ATCC 25923 and ATCC 700698), E. faecalis (ATCC11700), Escherichia coli (ATCC 25922), enterohemorrhagic E. coli (ATCC 43894), P. aeruginosa (ATCC 27853), Sh. flexneri (NCTC 8516), Salmonella enterica subsp. enterica (ATCC 14028), and clinical isolates of S. aureus, E. faecalis, E. faecium, E. coli, and P. aeruginosa collected from the Dr. Faghihi Hospital (Shiraz, Iran) were also determined in this study. The susceptibility of all clinical isolates of bacteria and fungi against select antibiotics were examined by microdilution and disk diffusion methods. [16],[17]

Determination of minimum inhibitory concentration

MICs were determined using the broth microdilution method recommended by the CLSI with some modifications. [16],[17] Briefly, for determination of antimicrobial activities against yeast, serial dilutions of the EOs (0.007-32.0 μL/mL) were prepared in 96-well microtiter plates using RPMI-1640 media (Sigma, St. Louis, MO, USA) buffered with MOPS (Sigma). To determine the antibacterial activities, serial dilutions of the EOs (0.03-128.0 μL/mL) were prepared in Muller-Hinton media (Merck, Darmstadt, Germany). Test yeasts or bacteria strains were suspended in media and the cell densities were adjusted to 0.5 McFarland standards at 530 nm wavelength using a spectrophotometeric method (this yields stock suspension of 1-5 × 10 6 cells/mL for yeasts and 1-1.5 Χ 10 8 cells/mL for bacteria). Working inoculums (0.1 mL) was added to the microtiter plates, which were incubated in a humid atmosphere at 30°C for 24-48 h (yeast) or at 37°C for 24 h (bacteria). Uninoculated medium (200 μL) was included as a sterility control (blank). In addition, growth controls (medium with inoculums but without EO) were also included. The growth in each well was compared with that of the growth in the control well. MICs were visually determined and defined as the lowest concentration of the EO produced ≥50% growth inhibition for fungi and ≥95% growth reduction for bacteria compared with the growth in the control well. Each experiment was performed in triplicate.

In addition, media from wells with fungi showing no visible growth were further cultured on Sabouraud dextrose agar (Merck, Darmstadt, Germany) and from wells with bacteria showing no visible growth on Muller-Hinton agar (Merck, Darmstadt, Germany) to determine the minimum fungicidal concentration (MFC) and minimum bactericidal concentration (MBC). MBCs and MFCs were determined as the lowest concentration yielding no more than 4 colonies, which corresponds to a mortality of 98% of the microbes in the initial inoculums.

   Results Top

The qualitative and quantitative compositions of the EOs of the full flowering, aerial parts of Z. multiflora ECTPs are presented in [Table 1]. GC-MS analyses showed that the main constituents of the EOs from ECTPs A and B were carvacrol (82.7%) and thymol-carvacrol (38.88%/27.16%), whereas that of ECTP C was linalool (87.35%).
Table 1: Chemical components (%) of the essential oils distilled from 3 ecotypes of Zataria multiflora

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The antibacterial activities of Z. multiflora EOs against the tested bacteria are shown in [Table 2]. The EOs inhibited the growth of all Gram-positive cocci at concentrations of 0.12-4 μL/mL. Furthermore, the EOs exhibited bactericidal activity (MBC) for all of the above-mentioned Gram-positive cocci at concentrations ranging from 1 to 8 μL/mL. Among the studied EOs, ECTP A had the highest inhibitory effect against the Gram-positive cocci with minimum inhibitory concentrations (MICs) about half of those of ECTPs B and C. No significant differences in inhibitory concentrations were found between antibiotic-resistant and -susceptible strains. All of the E. coli strains were susceptible to Z. multiflora EOs at concentrations of 0.12-8 μL/mL, while the EOs only inhibited the growth of about half of the isolates of P. aeruginosa at concentrations of 2-128 μL/mL. In addition, the EOs had bactericidal activity against all of the strains of E. coli and some strains of P. aeruginosa at concentrations of 0.12-8 μL/mL and 4-128 μL/mL, respectively.
Table 2: Antibacterial activity (MIC and MBC) of essential oils distilled from Zataria multiflora's ecotypes

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The antimicrobial activities of Z. multiflora EOs against yeasts are shown in [Table 3]. For the clinical and standard yeasts tested, the MICs for the EOs were 0.003-0.5 μL/mL. Among the examined EOs, ECTP A had the highest fungicidal activity with MFC values ranging from 0.03 to 0.25 μL/mL, followed by ECTP B (MFC: 0.03-1 μL/mL) and ECTP C (MFC: 0.25-4 μL/mL).
Table 3: Antifungal activity (MIC and MFC) of the essential oils of Zataria multiflora's ecotypes

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   Discussion Top

The composition of the EOs may vary greatly depending on the geographic region from which they are collected. The chemical composition of Z. multiflora EOs has already been reported. [1],[3],[18],[19] Based on GC-MS analyses of the oils collected from different regions of Fars province in this study, 3 distinct chemotypes were identified. Similar to the majority of earlier studies, [9],[19] we found carvacrol (82.7%) as the major ingredient of the EO extracted from the aerial parts of Z. multiflora collected from the Lamerd region (ECTP A). The higher concentration of carvacrol in this study as compared to those of previous reports [9],[19] may reflect variations due to geographical location from which the plants were collected. Alternatively, it may be due to differences in collecting fresh plants from fields as was done in this study vs purchasing them from herbal stores as in some studies, which may lead to the loss of parts of their volatile compounds.

Similar to previous reports, [1],[3],[20] a combination of 2 phenolic compounds, including carvacrol and thymol as the main constituents, was identified in the Z. multiflora EO collected from the Darab region (ECTP B), whereas very low amounts of thymol (<0.1%) were detected in the EO from the Lamerd region (ECTP A). Moreover, we found linalool (87.35%) as the main component of the EO collected from the Zarghan region (ECTP C). The identification of a linalool chemotype (ECTP C) in this study is in agreement with the study by Mohagheghzadeh et al. that reported high concentrations of linalool (60.39%) and linalyl acetate (8.55%) in the Z. multiflora EO collected from Kolahghazi (near Isfahan, Iran), which introduced an alcoholic chemotype as well as phenolic ones. [21]

Candida species are associated with mucocutaneous infections and currently are considered as the fourth most common causes of bloodstream infections. During the past several years, resistance to traditional triazole antifungal drugs, such as itraconazole and fluconazole, among clinical isolates of Candida has increased dramatically, justifying demands for novel antifungals. [22] The MICs and MFCs of the EOs of the 3 ECTPs of Z. multiflora were determined for the examined yeasts, showing strong anti-Candida activities with MIC values ranging from 0.007 to 0.5 μL/mL. This finding is similar to that of the study by Mahboubi et al. who reported strong anti-Candida activity of Z. multiflora EO with high thymol and carvacrol concentrations. [20] The lower MICs and MFCs of the examined EOs in this study as compared with that of a previous report [23] may be due to differences in the oil constituents or in the method used to assay antimicrobial activity (they used a macrodilution method and Sabouraud dextrose broth instead of a microdilution method and RPMI). Although the EO concentrations that caused inhibition were higher than those of antifungal drugs, the results are of interest because the EO is a mixture of components and not a pure compound. [23] As the 0.1% Z. multiflora cream was used successfully in the treatment of vaginal candidiasis, [24] the tested EOs may be potentially valuable as natural treatments of mucocutaneous candidiasis and geotrichosis.

Based on previous epidemiologic studies, E. coli O157:H7 accounts for many food-borne outbreaks in different countries. In this study, the EOs inhibited the growth of this strain at concentrations of 0.12, 0.25, and 2 μL/mL for ECTPs A, B, and C, respectively, which can be best compared to the study reported by Fazlara et al. on the same strain. [6] Similar to the study by Abbasgholizadeh et al., the EOs showed bactericidal effects against the clinical isolates of TGCsR and TGCsS E. coli at concentrations ranging from 0.12 to 8 μL/mL. [25] In addition, the EOs exhibited inhibitory and bactericidal activities against S. entrica and Sh. flexneri at concentrations ranging from >0.12 to 2 μL/mL. The antibacterial activity of ECTP B in this study is most comparable to that of Dakhili et al. who reported significant antibacterial effects against S. Typhimurium from the EO of Z. multiflora, which is rich in thymol and carvarole. [26] Moreover, they reported that Z. multiflora EO had strong antibacterial activity against S. Typhimurium, which is most comparable with the ECTP B in the present study. [26]

Staphylococcus aureus is one of the 4 most common causes of nosocomial infections, often causing postsurgical wound contamination. It is also considered as one of the main etiologic agents of food-borne infections. [11] In addition, there is major concern about this species due to the fast development of methicillin resistance. Similar to previous reports, [27] the growth of the standard and clinical isolates of MRSAs and MSSAs was inhibited by ECTPs A, B, and C of Z. multiflora EOs at concentrations of 0.55 to 1.41 μL/mL, respectively. In another study, Mahboubi and Ghazian found a chemotype of the Z. multiflora oil rich in thymol/carvacrol that significantly inhibited the growth of both MRSA and MSSA at concentrations ranging from 0.06 to 1 μL/mL, which is comparable to the ECTP B results found in this study. [18] Interestingly, the tested EOs had bactericidal activities and killed all of the S. aureus at concentrations less than 8 μL/mL. Moreover, it has been shown that the EO significantly prevented production of staphylococcal enterotoxin C during the manufacturing process of white brined cheese at concentrations as low as 5 μL/100 mL[28] ; hence, it might be used as a preservative additive in the food industry.

Vancomycin-resistant E. faecium is a problematic pathogen with few treatment options. All the tested ECTPs exhibited strong antimicrobial activities against vancomycin-resistant E. faecium as well as both vancomycin-resistant E. faecalis (VREf) and vancomycin-sensitive E. faecalis (VSEf). The MICs of ECTP A of Z. multiflora EO against VREfs and VSEfs in this study were much lower than those reported by Ravanshad et al., which used commercial EO and a macrodilution method. [29]

Among Gram-negative bacteria, P. aeruginosa appears to be the least sensitive to the EOs. In the present study, the tested EOs (ECTPs of Z. multiflora) killed 30%-50% of the susceptible and multidrug-resistant strains of P. aeruginosa at concentrations of up to 128 μL/mL (with MIC values ranging from 32 to 128 μL/mL).

The MICs and MBCs of the EOs against the examined Gram-negative bacteria were almost the same, whereas the MBCs of Gram-positive bacteria were 2-4 times higher than their corresponding MICs. One of the main characteristics of EOs is their hydrophobicity, which enables their incorporation into the cell membrane. [17] Among the tested ECTPs, ECTP A had the lowest MICs followed by ECTP B and ECTP C against both susceptible and resistant strains. ECTPs A and B were both rich in phenolic monoterpenes, including carvacrol and thymol. It has been shown that these phenolic monoterpenes have hydroxyl groups at different positions around the phenolic ring and exhibit their antimicrobial activities through disruption of the cytoplasmic membrane, which leads to leakage of ions and ATP. [30]

   Conclusion Top

Among the studied ECTPs, ECTP A with high concentration of carvacrol showed better antimicrobial activities than ECTP B and C. As the food industry tends to reduce the use of chemical preservatives in the food products, the EO of Z. multiflora with potential active antimicrobial properties might be considered as a natural source for the maintenance or extension of the shelf life of products. In addition, delectable taste of the EO at the concentrations needed for antimicrobial properties was a bonus to its antimicrobial effects. On the other hand, these EOs might also be considered for developing antibiotics and disinfectants for controlling infections caused by nosocomial pathogens. As these tests have all been done in vitro, the next step maybe is to further investigate in animal models to see if infection can be inhibited by these EOs.

   References Top

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20.Mahboubi M, Feizabadi MM, Safara M. Antifungal activity of essential oils from Zataria multiflora, Rosmarinus officinalis, Lavandula stoechas, Artemisia sieberi Besser and Pelargonium graveolens against clinical isolates of Candida albicans. Pharmacog Mag 2008;4:15-8.   Back to cited text no. 20
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25.Abbasgholizadeh N, Ettehad GH, Arab A, Nemati A, Barak M, Pirzadeh A, et al. Antibacterial effects of Zataria multiflora Boiss (Shiraz organo essence) on Enterobacteriaceae species. Res J Biol Sci 2008;3:345-7.  Back to cited text no. 25
26.Dakhili M, Zahraei Salehi T, Torabi Goodarzi M, Khavari A. Evaluation of antimicrobial effects of 4 medicinal plants against Salmonella typhymurium and comparision them with common antibiotics in veterinary medicine. J Med Plants 2006;5:21-6.   Back to cited text no. 26
27.Zahraei MT, Vojgani M, Bayat M, Tarshizi H, Akhoundzadeh Bastia A. Determination of minimum inhibitory concenteration (MIC) of extract of Zataria multiflora against the clinical isolates of Streptococcus agalactiae, Staphylococcus aureus and E. coli. J Vet Res 2005;60:107- 10.   Back to cited text no. 27
28.Parsaeimehr M, Akhondzadeh Bastia A, Radmehr B, Misaghi A, Abbasifar A, Ghity K, et al. Effect of Zataria multiflora Boiss. essential oil, nisin, and their combination on the production of enterotoxin C and alpha-hemolysin by Staphylococcus aureus. Foodborne Pathog Dis 2010;7:299-305.  Back to cited text no. 28
29.Ravanshad Sh, Basiri E, Dastgheib B. Antimicrobial activity of different consenterations of essential oil of Zataria multiflora on Enterococcus faecalis. Shiraz Univ Dent J 2007;8:28-36.  Back to cited text no. 29
30.Burt S. Essential oils: Their antibacterial properties and potential applications in foods-a review Int J Food Microbiol 2004;94:223-53.  Back to cited text no. 30


  [Table 1], [Table 2], [Table 3]

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Kah Min Yap, Mahendran Sekar, Shivkanya Fuloria, Yuan Seng Wu, Siew Hua Gan, Nur Najihah Izzati Mat Rani, Vetriselvan Subramaniyan, Chandrakant Kokare, Pei Teng Lum, M Yasmin Begum, Shankar Mani, Dhanalekshmi Unnikrishnan Meenakshi, Kathiresan V Sathasivam, Neeraj Kumar Fuloria
International Journal of Nanomedicine. 2021; Volume 16: 7891
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7 Nanoencapsulation enhances the contact toxicity of Eucalyptus globulus Labill and Zataria multiflora Boiss essential oils against the third instar larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)
Lena Emamjomeh, Sohrab Imani, Khalil Talebi Jahromi, Saeed Moharramipour
International Journal of Pest Management. 2021; : 1
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8 Effect of ethanolic extract of Zataria multiflora Boiss (Shiraz thyme extract) on the kidney and spleen tissues of mice infected with visceral candidiasis and the stimulation of Th1, Th17, and Treg immune cells
Mansour Bayat, Niloofar Hassannejad, Hadi Ghazanfari
Reviews in Medical Microbiology. 2020; 31(4): 201
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9 Nanofibrous cellulose acetate/gelatin wound dressing endowed with antibacterial and healing efficacy using nanoemulsion of Zataria multiflora
Hossein Farahani, Aboulfazl Barati, Mohammad Arjomandzadegan, Elham Vatankhah
International Journal of Biological Macromolecules. 2020; 162: 762
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10 Antimicrobial Effects of Zataria multiflora and Ocimum basilicum on Escherichia coli O157:H7 During Ripening of Traditional Lighvan Cheese
Mohammad B. Zendeh, Vadood Razavilar, Hamid Mirzaei, Khosrow Mohammadi
Current Nutrition & Food Science. 2020; 16(3): 373
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11 The Effect of Adding Thyme Extracts on Microbiological, Chemical and Sensory Characteristics of Yogurt
Sarmad Ghazi Al-Shawi, Haider Ibrahim Ali, Zena Kadhim Al-Younis
Journal of Pure and Applied Microbiology. 2020; 14(2): 1367
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12 Biological Activities of Essential Oils from Leaves of Paramignya trimera (Oliv.) Guillaum and Limnocitrus littoralis (Miq.) Swingle
Nhan Trong Le, Duc Viet Ho, Tuan Quoc Doan, Anh Tuan Le, Ain Raal, Donatella Usai, Giuseppina Sanna, Antonio Carta, Paola Rappelli, Nicia Diaz, Piero Cappuccinelli, Stefania Zanetti, Hoai Thi Nguyen, Matthew Gavino Donadu
Antibiotics. 2020; 9(4): 207
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13 Phytochemical Compositions and Biological Activities of Essential Oils from the Leaves, Rhizomes and Whole Plant of Hornstedtia bella Škornick
Matthew Gavino Donadu, Nhan Trong Le, Duc Viet Ho, Tuan Quoc Doan, Anh Tuan Le, Ain Raal, Marianna Usai, Mauro Marchetti, Giuseppina Sanna, Silvia Madeddu, Paola Rappelli, Nicia Diaz, Paola Molicotti, Antonio Carta, Sandra Piras, Donatella Usai, Hoai Thi Nguyen, Piero Cappuccinelli, Stefania Zanetti
Antibiotics. 2020; 9(6): 334
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14 Plant-Based Phytochemicals as Possible Alternative to Antibiotics in Combating Bacterial Drug Resistance
Hana Mohammed Al AlSheikh, Insha Sultan, Vijay Kumar, Irfan A. Rather, Hashem Al-Sheikh, Arif Tasleem Jan, Qazi Mohd Rizwanul Haq
Antibiotics. 2020; 9(8): 480
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15 The Possible Therapeutic Effects of Some Medicinal Plants for Chronic Cough in Children
S. Gholamreza Mortazavi Moghaddam, Majid Kianmehr, Mohammad Reza Khazdair, Andresa A. Berretta
Evidence-Based Complementary and Alternative Medicine. 2020; 2020: 1
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16 Ultrasound-assisted extraction of antimicrobial compounds from Thymus daenensis and Silybum marianum: Antimicrobial activity with and without the presence of natural silver nanoparticles
Mohammad Safarpoor, Mehrorang Ghaedi, Arash Asfaram, Masoumeh Yousefi-Nejad, Hamedreza Javadian, Hossein Zare Khafri, Marzieh Bagherinasab
Ultrasonics Sonochemistry. 2018; 42: 76
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17 Zein films and coatings as carriers and release systems of Zataria multiflora Boiss. essential oil for antimicrobial food packaging
Mahboobeb Kashiri,Josep P. Cerisuelo,Irene Domínguez,Gracia López-Carballo,Virginia Muriel-Gallet,Rafael Gavara,Pilar Hernández-Muñoz
Food Hydrocolloids. 2017; 70: 260
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18 Chemical Composition and Antimicrobial Activities of Three Satureja Species Against Food-borne Pathogens
Mohammad Jamal Saharkhiz,Kamiar Zomorodian,Azin Taban,Keyvan Pakshir,Keyvan Heshmati,Mohammad Javad Rahimi
Journal of Essential Oil Bearing Plants. 2016; 19(8): 1984
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19 Comparing the Effect of Garlic, Zataria multiflora and Clotrimazole Vaginal Cream 2% on Improvement of Fungal Vaginitis: A Randomized Controlled Trial
Azizeh Farshbaf-Khalili,Behnam Mohammadi-Ghalehbin,Mahnaz Shahnazi,Soltan Asghari,Yusef Javadzadeh,Payman Azghani
Iranian Red Crescent Medical Journal. 2016; Inpress(Inpress)
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20 Ecotype-correlated variations in germination and seedling growth ofZataria multiflora
Hossein Sadeghi,Zahra Robati
Botany. 2016; 94(10): 975
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21 Variability in Zataria multiflora Bioss. essential oil of twelve populations from Fars province, Iran
Hossein Sadeghi,Zahra Robati,Mohammad Jamal Saharkhiz
Industrial Crops and Products. 2015; 67: 221
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22 Chemical Compositions and Antimicrobial Activities of Ocimum sanctum L. Essential Oils at Different Harvest Stages
Mohammad Jamal Saharkhiz,Amir Alam Kamyab,Narges Khatoon Kazerani,Kamiar Zomorodian,Keyvan Pakshir,Mohammad Javad Rahimi
Jundishapur Journal of Microbiology. 2014; 8(1)
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23 Effects of Zataria Multi-Flora, Shirazi thyme, on the Severity of Premenstrual Syndrome
Marzieh Sodouri,Negin Masoudi Alavi,Nahid Fathizadeh,Mohsen Taghizadeh,Zohreh Azarbad,Mohammadreza Memarzadeh
Nursing and Midwifery Studies. 2013; 2(2): 57
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24 The Efficacy ofSatureja khuzistanicaEssential Oil Treatment in ReducingEscherichia coli O157:H7 Load on Alfalfa Seeds Prior to Sprouting
Azin Taban,Mohammad Javad Rahimi,Mohammad Jamal Saharkhiz,Javad Hadian,Kamiar Zomorodian
Journal of Food Safety. 2013; 33(2): 121
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25 Zataria multiflora Boiss. (Shirazi thyme)—An ancient condiment with modern pharmaceutical uses
Hassan Sajed,Amirhossein Sahebkar,Mehrdad Iranshahi
Journal of Ethnopharmacology. 2013; 145(3): 686
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26 Scolicidal effectiveness of essential oil from Zataria multiflora and Ferula assafoetida: disparity between phenolic monoterpenes and disulphide compounds
Gholamreza Kavoosi,Amin Mahammadi Purfard
Comparative Clinical Pathology. 2013; 22(5): 999
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27 The Efficacy of Satureja khuzistanica Essential Oil Treatment in Reducing Escherichia coliO157: H7 Load on Alfalfa Seeds Prior to Sprouting
Taban, A. and Rahimi, M.J. and Saharkhiz, M.J. and Hadian, J. and Zomorodian, K.
Journal of Food Safety. 2013; 33(2): 121-127
28 Zataria multiflora Boiss. (Shirazi thyme) - An ancient condiment with modern pharmaceutical uses
Sajed, H. and Sahebkar, A. and Iranshahi, M.
Journal of Ethnopharmacology. 2013; 145(3): 686-698
29 Radical scavenging properties of essential oils from Zataria multiflora and Ferula assafoetida
Kavoosi, G. and Purfard, A.M. and Aram, F.
Asian Pacific Journal of Tropical Biomedicine. 2012; 2(3 SUPPL.): S1351-S1356
30 Chemical composition and antibacterial activity of Zataria multiflora essential oil
Eftekhar, F. and Isazadeh, H. and Yousefzadi, M. and Hadian, J. and Ebrahimi, S.N.
Journal of Essential Oil-Bearing Plants. 2012; 15(3): 461-466
31 Chemical composition, radical scavenging, antibacterial and antifungal activities of zataria multiflora bioss essential oil and aqueous extract
Mahammadi Purfard, A. and Kavoosi, G.
Journal of Food Safety. 2012; 32(3): 326-332
32 Essential oils from aromatic herbs as antimicrobial agents
Solórzano-Santos, F., Miranda-Novales, M.G.
Current Opinion in Biotechnology. 2012; 23(2): 136-161
33 Comparative study of rosmarinic acid content in some plants of Labiatae family
Shekarchi, M., Hajimehdipoor, H., Saeidnia, S., Gohari, A.R., Hamedani, M.P.
Pharmacognosy Magazine. 2012; 8(29): 37-41
34 Essential oils from aromatic herbs as antimicrobial agents
Fortino Solórzano-Santos,Maria Guadalupe Miranda-Novales
Current Opinion in Biotechnology. 2012; 23(2): 136
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35 Radical scavenging properties of essential oils from Zataria multiflora and Ferula assafoetida
Gholamreza Kavoosi,Amin Mohammadi Purfard,Faezaneh Aram
Asian Pacific Journal of Tropical Biomedicine. 2012; 2(3): S1351
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Journal of Food Safety. 2012; 32(3): 326
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37 Chemical Composition and Antimicrobial Activities of Essential Oils from Nepeta cataria L. against Common Causes of Food-Borne Infections
Kamiar Zomorodian,Mohammad Jamal Saharkhiz,Samaneh Shariati,Keyvan Pakshir,Mohammad Javad Rahimi,Reza Khashei
ISRN Pharmaceutics. 2012; 2012: 1
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