| ORIGINAL ARTICLE |
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| Year : 2010 | Volume
: 6
| Issue : 23 | Page : 159-165 |
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Screening of molluscicidal strain against Oncomelania hupensis from the rhizosphere of medicinal plant Phytolacca acinosa Roxb
Danzhao Guo1, Jun Chen2, Xiangping Du2, Bangxing Han2
1 College of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu; College of Life Science and Technology, Henan Institute of Science and Technology, Henan, China
2 College of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu, China
Correspondence Address:
Jun Chen
College of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu
China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0973-1296.66928
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The research aimed to screen and exploit molluscicidal microorganisms against Oncomelania hupensis, from the rhizosphere of medicinal plant, Phytolacca acinosa Roxb., and one strain named as SL-30 was obtained with excellent molluscicidal activity. The freeze-dried powder of exocellular broth (EXB) of SL-30 could kill 100% of snails at a concentration of 48 mg/l for a submerged period of 24 h, with stabile molluscicidal activity at a temperature lower than 60°C; furthermore, it could be gradually degraded after exposure to illumination for 15 days. The freeze-dried powder of SL-30's EXB was safe to fresh fish and shrimp, even at a concentration beyond LC 90 of 24-h exposure period. The glycogen and total protein content of soft tissues of snails decreased after treating with SL-30's EXB, and glycogen content's decreasing rate had a linear relationship with molluscicidal activity. Finally, phylogenetic analysis based on ITS sequence showed that strain SL-30 had a higher similarity to Aspergillus fumigatus with bootstrap value 98%; accordingly, it was identified as a species of Aspergillus. |
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