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ORIGINAL ARTICLE
Year : 2010  |  Volume : 6  |  Issue : 22  |  Page : 111-115

Simultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD


1 Department of Biomaterials Engineering, School of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, Korea
2 TKM Converging Research Division, Korea Institute of Oriental Medicine, 483 Exporo, Yuseong-gu, Daejeon 305-811, Korea
3 Department of Biomaterials Engineering, School of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701; and Research Institute of Biotechnology, Kangwon National University, Chuncheon 200-701, Korea

Correspondence Address:
Choong Je Ma
Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University, Hyoja-2 Dong, Chuncheon 200-701
Korea
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Source of Support: Grant [K09040] from the Korea Institute of Oriental Medicine, Conflict of Interest: None


DOI: 10.4103/0973-1296.62896

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A HPLC-DAD method was established for the simultaneous evaluation of five bioactive compounds in Ssanghwa tang (SHT) including glycyrrhizin, paeoniflorin, cinnamic acid, decursin and 6-gingerol. These compounds were separated in less than 40 min using a Dionex C 18 column with a gradient elution system of water and methanol at a flow rate of 1 ml/min. Calibration curve of standard components presented excellent linear regression (R 2 > 0.9903) within the test range. Limit of detection and limit of quantification varied from 0.07 to 0.46 μg/ml and 0.13 to 1.11 μg/ml, respectively. The relative standard deviations (RSDs) of data of the intraday and interday experiments were less than 3.67 and 5.73%, respectively. The accuracy of recovery test ranged from 95.98 to 105.88% with RSD values 0.10- 4.82%.


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