Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
Advanced search 

Year : 2010  |  Volume : 6  |  Issue : 21  |  Page : 67-70 Table of Contents     

Acute oral toxicity of Pereskia bleo and Pereskia grandifolia in mice

1 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
2 Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
3 Center for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

Date of Submission10-Dec-2009
Date of Decision14-Dec-2009
Date of Web Publication13-Feb-2010

Correspondence Address:
A M Sri Nurestri
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1296.59969

Rights and Permissions

Pereskia bleo and Pereskia grandifolia, belonging to the botanical family Cactaceae, have been traditionally used by the locals in Malaysia for treatment of various ailments. The current study reports the outcome of acute oral toxicity investigation of Pereskia bleo and Pereskia grandifolia, on ICR mice. No mortalities or evidence of adverse effects have been observed in ICR mice following acute oral administration at the highest dose of 2500 mg/ kg crude extracts of Pereskia bleo and Pereskia grandifolia. This is the first report on the acute oral toxicity of Pereskia bleo and Pereskia grandifolia and the findings of this study are in agreement with those of in vitro experiments and thus provide scientific validation on the use of the leaves of Pereskia bleo and Pereskia grandifolia.

Keywords: Acute oral toxicity, Cactaceae, Pereskia bleo, Pereskia grandifolia

How to cite this article:
Sim K S, Sri Nurestri A M, Sinniah S K, Kim K H, Norhanom A W. Acute oral toxicity of Pereskia bleo and Pereskia grandifolia in mice. Phcog Mag 2010;6:67-70

How to cite this URL:
Sim K S, Sri Nurestri A M, Sinniah S K, Kim K H, Norhanom A W. Acute oral toxicity of Pereskia bleo and Pereskia grandifolia in mice. Phcog Mag [serial online] 2010 [cited 2022 Sep 26];6:67-70. Available from: http://www.phcog.com/text.asp?2010/6/21/67/59969

   Introduction Top

Medicinal herbs have always been used as traditional primary healthcare agents, especially in Asian countries. Over the last 20 years, rapid changes have been observed in the popular use of natural products from plant sources for maintainence of health and for alternative therapy, in Western countries. [1]

Pereskia bleo and Pereskia grandifolia, commonly known as 'Jarum Tujuh Bilah' in Malaysia belong to the botanical family Cactaceae. Pereskia bleo can be easily confused with Pereskia grandifolia because they are vegetatively similar. However, they can be easily distinguished by the leaves, flowers, and spines. Pereskia bleo has thinner, corrugated leaves, and orangish-red flowers, with shorter spines compared to Pereskia grandifolia. In contrast, Pereskia grandifolia has thicker, uncorrugated leaves, and pink to purplish-pink flowers, with longer and lesser spines. [2]

Both Pereskia bleo and Pereskia grandifolia have been used as natural remedies in cancer-related diseases, either eaten raw or taken as a concoction brewed from fresh plants. Both are believed to have anti-cancer, anti-tumor, anti-rheumatic, anti-ulcer, and anti-inflammatory properties. They are also used as remedies for the relief of headache, gastric pain, ulcers, hemorrhoids, atopic dermatitis, and for revitalizing the body. [3],[4],[5] In Panama, the locals use the whole plant of Pereskia bleo to treat gastrointestinal problems, [6] while in India, Pereskia grandifolia is used to reduce swelling. [7],[8]

Although Pereskia bleo and Pereskia grandifolia are reported to be used in a large number of Malaysian traditional medicine preparations, there is no published report on the study of acute oral toxicity of both Pereskia bleo and Pereskia grandifolia. The acute oral toxicity test is the simplest, and often the first toxicity test to be conducted on a sample. A single, high dose of the test sample is given to each experimental animal and the mortality is observed; death within the observation period (usually of 14 days duration), whether caused by natural death or humane killing, is studied. [9] The findings of this study corroborated the need for a safety study on both the Pereskia species used for primary health care in Malaysia. Such studies need to be carried out before the continued widespread use of some species provokes long-term and irreversible damage.

Previous cytotoxic investigations on Pereskia bleo[5],[10],[11] and Pereskia grandifolia[2] reported that both Pereskia bleo and Pereskia grandifolia had cytotoxic effects on various cancer cell lines, using in vitro cytotoxic activity assays, but did not show cytotoxicity against the normal cell line. [2],[11] If this also occurs in vivo, the use of this plant, by locals, for cancer treatment, would have scientific support.

   Materials and Methods Top

Plant sample collection and identification

The fresh leaves of Pereskia bleo and Pereskia grandifolia were collected from Petaling Jaya, Selangor, Malaysia in September 2006 and February 2007, respectively. They were identified by Professor Dr. Halijah Ibrahim of the Institute of Biological Sciences, Faculty of Science, University of Malaya, Malaysia, and voucher specimens were deposited in the herbarium of the Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia, with voucher numbers of SN01-06 (Pereskia bleo) and SN01-07 (Pereskia grandifolia).


Anhydrous sodium sulfate was purchased from the Sigma-Aldrich Company, while Tween 80 and methanol were obtained from the Merck Company.

Preparation of extracts

The crude extracts were prepared as previously described. [11] Briefly, the fresh leaves of Pereskia bleo and Pereskia grandifolia were washed, dried, and ground to a fine powder, using a blender. The dried, ground leaves were then soaked in methanol (1.5 L) for three days, at room temperature. The solvent-containing extract was then decantered, dried with anhydrous sodium sulfate, and filtered. The extraction of the ground leaves was further repeated (2x) with methanol (1.5 L each time). The filtrates from each extraction were combined and the excess solvent was evaporated (Buchi, Rotavapor, Switzerland) under reduced pressure, using a rotary evaporator, to give a dark green crude methanol extract.

Test species

The experiment was performed on healthy ICR mice (five weeks of age, body weight 23-28 g), obtained from the Laboratory Animal Center, Faculty of Medicine, University of Malaya. The female mice were confirmed nulliparous and non-pregnant. The mice were assigned to five dosage groups and one control group with 10 mice (five male and five female) for each test group. The weight variation in the mice used did not exceed ± 20% SD of the mean body weight of each sex. The experimental procedures involving the animals were approved by the University of Malaya Animal Experimental Ethnics Committee [Ethical number: ISB/05/08/2009/SKS (R)] before commencing the study.

Procedure of acute oral toxicity

The acute oral toxicity of the crude methanol extracts of both the Pereskia species were evaluated in mice using the procedure described by the OECD (Organization for Economic Co-operation and Development), with some modifications. The mice were housed in suspended, stainless steel, wire-mesh cages in an experimental animal room. The temperature was maintained at 23 ± 3°C and the relative humidity was 50-60% before and after treatment with the extract. The animal room was artificially illuminated (fluorescent light) with an approximate 12-hour light/ dark cycle. The mice were acclimatized to the laboratory conditions for at least five days prior to commencement of the experiments. The mice were randomly selected for use in the study and marked to provide individual identification. Conventional mouse diets, with unlimited supply of drinking water, were available ad libitum, except during the fasting period. The mice were fasted approximately 12 hours prior to dosing, but they had free access to drinking water. Before and after treatment with the extract, the mice were caged in groups by sex and dose levels. The extracts were suspended in a vehicle (10% Tween-80 in distilled water). A stock concentration of 200 mg/ml was prepared and the mice were administrated with 0.2 ml of the extract for every 10 g of mice body weight. The mice were administered with doses of 500, 1000, 1500, 2000, and 2500 mg/kg of extracts. Food was started for the animals approximately three to four hours after dosing. The mice were observed carefully for any signs of toxicity in the first four hours after the treatment period, and daily thereafter for a period of 14 days. Observations for mortality, signs of illness, injury, pain, distress, allergic reactions, changes of outer appearance, behavioral alterations (i.e., ataxia, hyperactivity, hypoactivity), and general stimulation or sedation were conducted twice daily. The observations were recorded systematically; individual records were maintained for each mouse.

   Results and Discussion Top

Extraction yield of Pereskia bleo and Pereskia grandifolia

Solvent extraction is the most popular method used in sample preparation. The yields from methanol extracts of Pereskia bleo and Pereskia grandifolia are shown in [Table 1]. Before extraction, the plant material needs to be dried to avoid the presence of water in the extracts. The percentage of crude methanol extract yield is based on the weight of dried and ground plant materials. Methanol is used as the extraction solvent due to its polarity and its known ability to extract compounds such as, phenolics, flavonoids, and other polar materials. [12]

Acute oral toxicity assessment of Pereskia bleo and Pereskia grandifolia crude extracts

Investigation of acute toxicity is the first step in the toxicological analysis of herbal drugs. [13] Overall, animal models have a good predictability for human toxicities of around 70-80%. [14],[15] Generally, it is possible to get the first hints on complex toxicities by applying in vivo methods, as information on some toxic manifestations cannot be assessed by in vitro cytotoxicity methods. [16] Toxic manifestations that affect the entire organism such as pain, distress, allergic reactions, changes in outer appearance, behavioral alterations, and general stimulation or sedation can be detected by in vivo assays. However, the detection of effects on vital functions (cardiovascular, central nervous, and respiratory systems) is usually not assessed in acute toxicity studies.

Acute oral toxicity was undertaken in the present study to determine the safety parameters of the leaves of Pereskia bleo and Pereskia grandifolia. Mortality, clinical signs, gross findings, and body weights of mice were observed and measured for 14 days after the oral administration of crude methanol extracts to both Pereskia sp. The crude methanol extracts were used in this acute oral toxicity study to ensure that all components in the extract were included.

[Table 2] shows the results of the acute toxicity of the crude extracts of Pereskia bleo and Pereskia grandifolia. For all doses tested for crude methanol extracts of Pereskia bleo and Pereskia grandifolia, there were no deaths reported. Throughout the 14-day observation period, there were no significant changes in behavior (i.e., ataxia, hyperactivity, hypoactivity) in any of the mice, nor did they produce any variations in the general appearance. They gained weight with no adverse clinical signs of toxicity at any dose.

Traditionally, the aim of the acute oral toxicity study was the estimation of LD 50 . The LD 50 value - defined as the statistically derived dose, which when administered in an acute toxicity test, is expected to cause death in 50% of the treated animals in a given period - is currently the basis for toxicological classification of chemicals. For a classical LD 50 study, laboratory mice and rats are the species typically selected. Often both sexes must be used for regulatory purposes. [14]

As no deaths were found for all doses tested for crude methanol extracts of Pereskia bleo and Pereskia grandifolia, the LD 50 values of crude P. bleo and P. grandifolia extracts were >2500 mg/kg. This indicated that both Pereskia sp. did not cause any acute toxicity. According to the chemical labeling and classification of acute systemic toxicity, based on oral LD 50 values, which were recommended by OECD, [ 17],[18] the crude extracts of both Pereskia sp. were assigned to class 5 (LD 50 > 2000 mg/kg), which was termed as the lowest toxicity class (no label; unclassified). Oliver [19] pointed out that (i) the LD 50 value was not an absolute value, but was an inherently variable biological parameter that could not be described in terms of accuracy, but only of precision, (ii) the LD 50 value referred only to mortality and was illustrative of no other clinical expression of toxicity.

   Conclusion Top

In view of the increasing popular consumption of medicinal plants as alternative therapy, it is necessary to conduct research to support the therapeutic claims and also to ensure that the plants are indeed safe for human consumption. The present research findings have clearly met the objectives of the study. The result was in agreement with that of in vitro experiments, whereby, the crude extracts of Pereskia bleo and Pereskia grandifolia did not show cytotoxicity against normal MRC-5 cells. [2],[11] Based on the outcome of acute toxicity in experimental mice, the crude extracts of both Pereskia sp. could be regarded as safe in experimental mice. Further toxicity study over a longer period of time involving detection of effects on vital organ functions would ensure that the plants are safe for human consumption.

   Acknowledgment Top

This study was supported by research funding from the University of Malaya (PPP PS241/2008C) and the Fundamental Research Grant Scheme from the Ministry of Higher Education (FP040/2008C). We are also grateful to Mr V.T. Johgalingam for his technical support.

   References Top

1.Wills RB, Bone K, Morgan M. Herbal products: Active constituents, modes of action and quality control. Nutr Res Rev 2000;13:47-7.  Back to cited text no. 1  [PUBMED]  [FULLTEXT]  
2.Sri Nurestri AM, Sim KS, Norhanom AW. Phytochemical and cytotoxic investigations of Pereskia grandifolia Haw. (Cactaceae) leaves. J Biol Sci 2009;9:488-93.  Back to cited text no. 2      
3.Goh KL. Malaysian herbaceous plants. Malaysia: Advanco Press; 2002.  Back to cited text no. 3      
4.Rahmat A. Daun jarum tujuh bilah untuk barah, sakit kepala. Malaysia: Utusan Melayu (M) Bhd; 5 December 2004.  Back to cited text no. 4      
5.Tan ML, Sulaiman SF, Najimuddin N, Samian MR, Tengku Muhammad TS. Methanolic extract of Pereskia bleo (Kunth) DC: (Cactaceae) induces apoptosis in breast carcinoma, T47-D cell line. J Ethnopharmacol 2005;96:287-94.  Back to cited text no. 5      
6.Gupta MP, Monge A, Karikas GA, Lopez de Cerain A, Solis PN, De Leon E, et al. Screening of Panamanian medicinal plants for brine shrimp toxicity, crown gall tumor inhibition, cytotoxicity and DNA intercalation. Int J Pharmacog 1996;34:18-27.  Back to cited text no. 6      
7.Sahu NP, Banerji N, Chakravarti RN. A new saponin of oleanolic acid from Pereskia grandifolia. Phytochemistry 1974;13:529-30.  Back to cited text no. 7      
8.Anon. The wealth of India. Vol. VII. India: Council of Scientific and Industrial Research; 1969.  Back to cited text no. 8      
9.Stallard N. Optimal adaptive designs for acute oral toxicity assessment. J Statist Plann Inference 2006;136:1781-99.  Back to cited text no. 9      
10.Er HM, Chen E, Radhakrishnan. Anti-proliferative and mutagenic activities of aqueous and methanol extracts of leaves from Pereskia bleo (Kunth) DC leaves. J Ethnopharmacol 2007;113:448-56.  Back to cited text no. 10      
11.Sri Nurestri AM, Norhanom AW, Hashim Y, Sim KS, Hong SL, Lee GS, et al. Cytotoxic activity of Pereskia bleo (Cactaceae) against selected human cell lines. Int J Cancer Res 2008;4:20-7.  Back to cited text no. 11      
12.Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics on selected fruits, vegetables, and grain products. J Agric Food Chem 1998;46:4113-7.  Back to cited text no. 12      
13.Deciga-Campos M, Rivero-Cruz I, Arriaga-Alba M, Castaneda-Corral G, Angeles-Lopez GE, Navarrete A, et al. Acute toxicity and mutagenic activity of Mexican plants used in traditional medicine. J Ethnopharmacol 2007;110:334-42.  Back to cited text no. 13      
14.Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, et al. Concordance of the toxicity of pharmaceuticals in humans and in animals. Reg Toxicol Pharmacol 2000;32:56-67.  Back to cited text no. 14      
15.Kola I, Landis J. Can the pharmaceutical industry reduce attrition rates? Nat Rev Drug Discov 2004;3:711-6.  Back to cited text no. 15  [PUBMED]  [FULLTEXT]  
16.Ukelis U, Kramer PJ, Olejniczak K, Mueller SO. Replacement of in vivo acute oral toxicity studies by in vitro cytotoxicity methods: Opportunities, limits and regulatory status. Reg Toxicol Pharmacol 2008;51:108-18.  Back to cited text no. 16      
17.OECD (Organization for Economic Co-operation and Development). Harmonized Integrated Hazard Classification System for Human Health and Environmental Effects of Chemical Substances. Paris: OECD; 1998.  Back to cited text no. 17      
18.Walum E. Acute oral toxicity. Environ Health Perspect 1998;106:497-503.  Back to cited text no. 18  [PUBMED]  [FULLTEXT]  
19.Oliver JA. Opportunities for using fewer animals in acute toxicity studies. In: Chemicals testing and animal welfare. Sweden: The National Chemicals Inspectorate; 1986. p. 119-42.  Back to cited text no. 19      


  [Table 2]

  [Table 1]

This article has been cited by
1 GC-MS analysis of phytochemical compounds of Opuntia megarrhiza (Cactaceae), an endangered plant of Mexico
Madeleyne Cupido, Arturo De-Nova, María L. Guerrero-González, Francisco Javier Pérez-Vázquez, Karen Beatriz Méndez-Rodríguez, Pablo Delgado-Sánchez
PeerJ Organic Chemistry. 2022; 4: e5
[Pubmed] | [DOI]
2 Phytochemical profile, toxicological evaluation of Rhipsalis baccifera (Sol.) Stearn (Cactaceae) extract and their antitumor activity in Ehrlich carcinoma-bearing mice
Jéssica Alves Cavalcantea, Luiz da Silva Maia Neto, Anna Lígia de Castro Figueiredo, Weslley Oliveira, José Roberto Pimentel Cabral de Seixas, Caio César da Silva Guedes, Priscila Maria de Barros Rodrigues, Vanessa Silva de Almeida, Lucíola Abílio DM de Medeiros Rolim, Andrezo Adenilton Santos, Joicy Kelly Alves da Silva, Rômulo Carlos Dantas da Cruz, Raíra Justino Oliveira Costa, Taciana Michele de Lira Moura, Antônio Fernando Morais de Oliveira, Silvania Tavares Paz, Cleonice Régis de Figueiredo Neta, Ivone Antônia de Souza
Natural Resources for Human Health. 2021; 1(2): 63
[Pubmed] | [DOI]
3 Improvement of Bioactive Compound Levels, Antioxidant Activity, and Bioaccessibility of Carotenoids from Pereskia aculeata after Different Cooking Techniques
Bruna Vitória Neves, Leonardo M. de Souza Mesquita, Daniella Carisa Murador, Ana Isabel do Prado Cheberle, Anna Rafaela Cavalcante Braga, Adriana Zerlotti Mercadante, Veridiana Vera de Rosso
ACS Food Science & Technology. 2021; 1(7): 1285
[Pubmed] | [DOI]
4 Rheological and Functional Properties of Hydrocolloids from Pereskia bleo Leaves
Diana López-Barraza, Andres Ortega-Ramos, Edilbert Torregroza-Fuentes, Somaris E. Quintana, Luis A. García-Zapateiro
Fluids. 2021; 6(10): 349
[Pubmed] | [DOI]
5 Juice from leaves of cacti of the genus Pereskia: effect on the physiological parameters of Wistar rats
Débora Cristina Mariano Brasil, Rafaela Marchiori Mariano do Val, José Antônio de Souza Cruz Ramos, Martha Elisa Ferreira de Almeida
Ciência Animal Brasileira. 2020; 21
[Pubmed] | [DOI]
6 The application of phosphorus and potassium to increase drought tolerance in Pereskia bleo (Kunt) DC with proline and antioxidant indicators
Sulandjari, A T Sakya, D H Prahasto
IOP Conference Series: Earth and Environmental Science. 2020; 423(1): 012055
[Pubmed] | [DOI]
7 Bioactive compounds and health benefits of Pereskioideae and Cactoideae: A review
Tânia da Silveira Agostini-Costa
Food Chemistry. 2020; 327: 126961
[Pubmed] | [DOI]
8 Evaluation of acute, subacute oral toxicity and wound healing activity of mother plant and callus of Teucrium polium L. subsp. geyrii Maire from Algeria
H. Meguellati, S. Ouafi, S. Saad, N. Djemouai
South African Journal of Botany. 2019; 127: 25
[Pubmed] | [DOI]
9 Acute Toxicity and Cytotoxicity of Pereskia aculeata, a Highly Nutritious Cactaceae Plant
Debora O. Silva,Mauricio Seifert,Fabiana R. Nora,Vera L. Bobrowski,Rogerio A. Freitag,Heidi R. Kucera,Leonardo Nora,Nilesh W. Gaikwad
Journal of Medicinal Food. 2017; 20(4): 403
[Pubmed] | [DOI]
10 Pereskia aculeata Muller (Cactaceae) Leaves: Chemical Composition and Biological Activities
Lucèia Souza,Lucia Caputo,Ingrid Inchausti De Barros,Florinda Fratianni,Filomena Nazzaro,Vincenzo De Feo
International Journal of Molecular Sciences. 2016; 17(9): 1478
[Pubmed] | [DOI]
11 Pereskia aculeata: A plant food with antinociceptive activity
Nícolas de Castro Campos Pinto,Ana Paula do Nascimento Duque,Natália Ramos Pacheco,Renata de Freitas Mendes,Erick Vicente da Silva Motta,Paula Maria Quaglio Bellozi,Antônia Ribeiro,Marcos José Salvador,Elita Scio
Pharmaceutical Biology. 2015; 53(12): 1780
[Pubmed] | [DOI]
12 Pharmaco-toxic characterization of the aqueous extract from Pereskia grandifolia leaves
Henrique Souza Cesar Pedro,Henrique de Moura Oliveira Carlos,Marcussi Silvana
Journal of Medicinal Plants Research. 2015; 9(7): 216
[Pubmed] | [DOI]
13 Improvement of physiological parameters of rats subjected to hypercaloric diet, with the use of Pereskia grandifolia (Cactaceae) leaf flour
Martha Elisa Ferreira de Almeida,Anderson Assaid Simão,Angelita Duarte Corrêa,Regiane Victória de Barros Fernandes
Obesity Research & Clinical Practice. 2015;
[Pubmed] | [DOI]
14 Ethnobotanical survey of usage of fresh medicinal plants in Singapore
Yin-Yin Siew,Sogand Zareisedehizadeh,Wei-Guang Seetoh,Soek-Ying Neo,Chay-Hoon Tan,Hwee-Ling Koh
Journal of Ethnopharmacology. 2014; 155(3): 1450
[Pubmed] | [DOI]
15 The Biological Activities and Chemical Composition of Pereskia Species (Cactaceae)—A Review
Nícolas de Castro Campos Pinto,Elita Scio
Plant Foods for Human Nutrition. 2014;
[Pubmed] | [DOI]
16 A Review of Botanical Characteristics, Traditional Usage, Chemical Components, Pharmacological Activities, and Safety of Pereskia bleo (Kunth) DC
Sogand Zareisedehizadeh,Chay-Hoon Tan,Hwee-Ling Koh
Evidence-Based Complementary and Alternative Medicine. 2014; 2014: 1
[Pubmed] | [DOI]
17 Acute toxicity and the 28-day repeated dose study of a Siddha medicine Nuna Kadugu in rats
Ramaswamy Ramaswamy,Nettam Prathyusha,Ruthiramoorthi Saranya,Haridass Sumathy,Kutuva Mohanavalli,Raju Priya,Jayakothanda Venkhatesh,Chidambaram Babu,Kumarasamy Manickavasakam,Sadagopan Thanikachalam
BMC Complementary and Alternative Medicine. 2012; 12(1): 190
[Pubmed] | [DOI]
18 Involvement of arginine-vasopressin in the diuretic and hypotensive effects of Pereskia grandifolia Haw. (Cactaceae)
Caroline Calixto Kazama,Denise Thiemi Uchida,Karina Natally Canzi,Priscila de Souza,Sandra Crestani,Arquimedes Gasparotto Junior,Antonio Laverde Junior
Journal of Ethnopharmacology. 2012; 144(1): 86
[Pubmed] | [DOI]
19 Acute and subacute oral toxicity evaluation of Tephrosia purpurea extract in rodents
Talib Hussain,Sheeba Fareed,HH Siddiqui,M Vijaykumar,Chandana Venkateswara Rao
Asian Pacific Journal of Tropical Disease. 2012; 2(2): 129
[Pubmed] | [DOI]
20 Acute toxicity and the 28-day repeated dose study of a Siddha medicine Nuna Kadugu in rats
Ramaswamy, R.S. and Prathyusha, N. and Saranya, R. and Sumathy, H. and Mohanavalli, K.T. and Priya, R.J. and Venkhatesh, J.R. and Babu, C.S. and Manickavasakam, K. and Thanikachalam, S.
BMC Complementary and Alternative Medicine. 2012; 12(190)
21 Toxicological evaluation of Murraya paniculata (L.) leaves extract on rodents
Gautam, M.K. and Singh, A. and Rao, C.V. and Goel, R.K.
American Journal of Pharmacology and Toxicology. 2012; 7(2): 62-67
22 Acute and subacute oral toxicity evaluation of Tephrosia purpurea extract in rodents
Hussain, T. and Fareed, S. and Siddiqui, H.H. and Vijaykumar, M. and Rao, C.V.
Asian Pacific Journal of Tropical Disease. 2012; 2(2): 129-132
23 Utilization of cacti of the genus pereskia in the human diet in a municipality of minas gerais [Utilização de cactáceas do gênero pereskia na alimentação humana em um município de minas gerais]
de Almeida, M.E.F. and Corrêa, A.D.
Ciencia Rural. 2012; 42(4): 751-756
24 Cytotoxic and antioxidant activity of Pereskia aculeata Miller
Pinto, N.C.C. and dos Santos, R.C. and Machado, D.C. and Florêncio, J.R. and Fagundes, E.M.S. and Antinarelli, L.M.R. and Coimbra, E.S. and Ribeiro, A. and Scio, E.
Pharmacologyonline. 2012; 3: 63-69
25 Involvement of arginine-vasopressin in the diuretic and hypotensive effects of Pereskia grandifolia Haw. (Cactaceae)
Kazama, C.C. and Uchida, D.T. and Canzi, K.N. and De Souza, P. and Crestani, S. and Gasparotto Junior, A. and Laverde Junior, A.
Journal of Ethnopharmacology. 2012; 144(1): 86-93
26 Evaluation of acute and sub-acute toxicity of methanolic extract of caesalpinia bonducella (L) fleming
Pillaia, P.G., Suresh, P.
European Journal of Scientific Research. 2011; 53(3): 462-469
27 Pharmacognostical standardization and toxicity profile of the methanolic leaf extract of Plectranthus amboinicus (Lour) Spreng
Pillai, P.G. and Suresh, P. and Aggarwal, G. and Doshi, G. and Bhatia, V.
Journal of Applied Pharmaceutical Science. 2011; 1(2): 76-81
28 Acute and sub-chronic toxicity study of standardized extract of Fumaria indica in rodents
Singh, G.K., Kumar, V.
Journal of Ethnopharmacology. 2011; 134(3): 992-995
29 Acute and sub-chronic toxicity study of standardized extract of Fumaria indica in rodents
Gireesh Kumar Singh,Vikas Kumar
Journal of Ethnopharmacology. 2011; 134(3): 992
[Pubmed] | [DOI]
30 Cytotoxic activity of leaf and rhizome extracts of Alpinia scabra (Blume) Náves, a wild ginger from Peninsular Malaysia
Ibrahim, H., Sim, K.S., Syamsir, D.R., Nor, N.R.M., Sri Nurestri, A.M., Awang, K.
African Journal of Pharmacy and Pharmacology. 2010; 4(10): 708-711


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

  In this article
    Materials and Me...
    Results and Disc...
    Article Figures
    Article Tables

 Article Access Statistics
    PDF Downloaded317    
    Comments [Add]    
    Cited by others 30    

Recommend this journal