Anti-mycobacterial Activity of Extracts from Plants used in Mexico for the Treatment of Infectious Diseases
Samuel Estrada-Soto1, Valeria Arteaga-Gonzalez1, Patricia Castillo-Espana2, Gloria Maria Molina-Salinas3, Salvador Said-Fernandez3, Gabriel Navarrete-Vazquez1
1 Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Col. Chamilpa, 62209, Cuernavaca, Morelos, Mexico
2 Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Col. Chamilpa, 62209, Cuernavaca, Morelos, Mexico
3 División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, IMSS, San Luis Potosí y Dos de Abril, Colonia Independencia 64720, Monterrey, Nuevo León, Mexico
Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Col. Chamilpa, 62209, Cuernavaca, Morelos
Source of Support: None, Conflict of Interest: None
Twenty seven extracts from eight medicinal plants used in Morelos, Mexico, for the treatment of several infectious diseases were screened for their in vitro anti-Mycobacterium tuberculosis (anti-MTB) activity on H37Rv strain of Mycobacterium tuberculosis (MTB), using the microplate Atamar assay test. Extracts (hexane, dichloromethane and methanol) from wild specimen of Valerians edulis showed the most significant (p<0.05) activity against this strain (MICs values of 3.125, 25.0 and 50.0 μg/mL, respectively). On the other hand, hexanic and dichlorometanic extracts from in vitro-cultivated species of V. edulis also showed significantly activity against of M. tuberculosis (MICs values of 12.5 and 50.0 μg/mL, respectively). Most active extract (hexanic) obtained from V. edulis was also assayed against the drug-resistant strain (CIBIN 99), and the MIC value was 50 μmL. A qualitative TLC analysis of extracts from wild and in vitro-cultivated species of V. edutis showed that both content different chemical constituents. So, this last result explains the difference between anti-MTB activities of the extracts. Finally, dichloromethanic extracts from Lepechinia caulescens also showed a significantly activity against of M. tuberculosis (MIC 100 μglmL). In conclusion, V. edutis and L. caulescens hexanic extracts are sources for the isolation of new or known compounds that could be used as leads for development of potential anti-MTB drugs.