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  Indian J Med Microbiol
 

Figure 5: (a) Induction of apoptosis, showing the expected ladder pattern (100–1000 pb) on the left. On the right, DNA fragmentation of HeLa cells produced by: (1) the vehicle control, (2) F4 (obtained from the hexane extract of Rauvolfia tetraphylla leaves) and (3) cisplatin. (b) The translocation of phosphatidylserine was measured by flow cytometry. Q1 displays PI (*) annexin V (−), representing necrosis. Q2 portrays PI (*) annexin V (*), representing late apoptosis. Q3 depicts PI (−) annexin V (−), representing healthy cells. Q4 illustrates PI (−) annexin V (*), representing early apoptosis

Figure 5: (a) Induction of apoptosis, showing the expected ladder pattern (100–1000 pb) on the left. On the right, DNA fragmentation of HeLa cells produced by: (1) the vehicle control, (2) F4 (obtained from the hexane extract of <i>Rauvolfia tetraphylla</i> leaves) and (3) cisplatin. (b) The translocation of phosphatidylserine was measured by flow cytometry. Q1 displays PI (*) annexin V (−), representing necrosis. Q2 portrays PI (*) annexin V (*), representing late apoptosis. Q3 depicts PI (−) annexin V (−), representing healthy cells. Q4 illustrates PI (−) annexin V (*), representing early apoptosis