Close
  Indian J Med Microbiol
 

Figure 3: Changes of the mRNA and protein levels of Shh, Ptch, Smo and glioma-associated oncogene homolog 1 genes in the breast cancer stem-like cells after rosmarinic acid treatment. (a) Quantitative reverse transcriptase polymerase chain reactionwas used to compare the changes of the mRNA levels after 0, 10, 30, 90, 270 and 810 µmol/L rosmarinic acid treatment for 48 h. β-actin was used as an internal standard to calculate the relative amounts (2–ΔΔCT) of the mRNA of genes of interests. (b) Immunoblot analysis was used to compare the protein levels after 0, 10, 30, 90, 270 and 810 µmol/L rosmarinic acid treatment for 48 h. Left, the representative immunoblots. Right, protein levels of the immunoblot analysis were quantified and normalized to controls. *P < 0.05 compared to the control

Figure 3: Changes of the mRNA and protein levels of Shh, Ptch, Smo and glioma-associated oncogene homolog 1 genes in the breast cancer stem-like cells after rosmarinic acid treatment. (a) Quantitative reverse transcriptase polymerase chain reactionwas used to compare the changes of the mRNA levels after 0, 10, 30, 90, 270 and 810 µmol/L rosmarinic acid treatment for 48 h. β-actin was used as an internal standard to calculate the relative amounts (2<sup>–ΔΔCT</sup>) of the mRNA of genes of interests. (b) Immunoblot analysis was used to compare the protein levels after 0, 10, 30, 90, 270 and 810 µmol/L rosmarinic acid treatment for 48 h. Left, the representative immunoblots. Right, protein levels of the immunoblot analysis were quantified and normalized to controls. *<i>P</i> < 0.05 compared to the control