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  Indian J Med Microbiol
 

Figure 4: Effects of PN extract on Reactive oxygen species formation, caspase-3 activity and protein-related cell growth or cell death of breast cancer cells (a-c). After MCF-7 cells were treated with 0–500 µg/mL PN extract with DHE-probe for 90 min in dark and analyzed Reactive oxygen species formation by measured fluorescent intensity. Cells were treated with 0–100 µg/mL PN extract for 24 h and measured caspase-3 activity by the kits. Cells were incubated with 0–50 µg/mL PN extract for 24 h and measured protein expression by Western blotting. Data represent mean ± standard error of the mean of three independent experiments. *P < 0.05 versus control. PN: Piper nigrum

Figure 4: Effects of PN extract on Reactive oxygen species formation, caspase-3 activity and protein-related cell growth or cell death of breast cancer cells (a-c). After MCF-7 cells were treated with 0–500 µg/mL PN extract with DHE-probe for 90 min in dark and analyzed Reactive oxygen species formation by measured fluorescent intensity. Cells were treated with 0–100 µg/mL PN extract for 24 h and measured caspase-3 activity by the kits. Cells were incubated with 0–50 µg/mL PN extract for 24 h and measured protein expression by Western blotting. Data represent mean ± standard error of the mean of three independent experiments. *<i>P</i> < 0.05 versus control. PN: <i>Piper nigrum</i>