Close
  Indian J Med Microbiol
 

Figure 3: Effects of PN extract on viability, cell cycle progression and colony formation of breast cancer cells (a-e). MCF-7 cells were treated with 0–100 µg/mL PN extract for 24–48 h and then analyzed cancer cells death by sulforhodamine B. Cells were treated with 0–25 µg/mL PN extract for 24 h and then analyzed cell cycle arrest by flow cytometry. Cells were treated with 0–100 µg/mL PN extract for 24 h and then added the new complete medium every 2 days for 2 weeks and analyzed colony formation. Data represent mean ± standard error of the mean of three independent experiments. *P < 0.05 versus control. PN: Piper nigrum

Figure 3: Effects of PN extract on viability, cell cycle progression and colony formation of breast cancer cells (a-e). MCF-7 cells were treated with 0–100 µg/mL PN extract for 24–48 h and then analyzed cancer cells death by sulforhodamine B. Cells were treated with 0–25 µg/mL PN extract for 24 h and then analyzed cell cycle arrest by flow cytometry. Cells were treated with 0–100 µg/mL PN extract for 24 h and then added the new complete medium every 2 days for 2 weeks and analyzed colony formation. Data represent mean ± standard error of the mean of three independent experiments. *<i>P</i> < 0.05 versus control. PN: <i>Piper nigrum</i>