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  Indian J Med Microbiol
 

Figure 2: Effects of PN extract on gene and protein expressions of Rac1, RhoA, and Cdc42 (a and b). MCF-7 cells were treated with 25–50 µg/mL PN extract for 24 h and then analyzed gene and protein expression. Reverse transcription polymerase chain reaction and Western blotting were carried out for targeted gene and protein expression, respectively. Data represent mean ± standard error of the mean of three independent experiments. *P < 0.05 versus control. PN: Piper nigrum

Figure 2: Effects of PN extract on gene and protein expressions of Rac1, RhoA, and Cdc42 (a and b). MCF-7 cells were treated with 25–50 µg/mL PN extract for 24 h and then analyzed gene and protein expression. Reverse transcription polymerase chain reaction and Western blotting were carried out for targeted gene and protein expression, respectively. Data represent mean ± standard error of the mean of three independent experiments. *<i>P</i> < 0.05 versus control. PN: <i>Piper nigrum</i>