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  Indian J Med Microbiol
 

Figure 3: EE treatments influenced on the mRNA expression of apoptosis-related genes in LCC cells. The cells were treated with the indicated concentrations of EE for 24 h. (a) The mRNA expression levels of caspase-9, Bax, and Bcl-2 were analyzed by quantitative real-time PCR. (b) The protein expression levels of caspase-9, Bax, and Bcl-2 were analyzed by Western blot. β-actin was used as a control. Data were normalized to the mean value of the control group and presented as the mean ± SD *P < 0.05, **P < 0.01 versus control. PCR: polymerase chain reaction; SD: Standard deviation; LCC: Large cell carcinoma; EE: Euphorbiaceae extract

Figure 3: EE treatments influenced on the mRNA expression of apoptosis-related genes in LCC cells. The cells were treated with the indicated concentrations of EE for 24 h. (a) The mRNA expression levels of caspase-9, Bax, and Bcl-2 were analyzed by quantitative real-time PCR. (b) The protein expression levels of caspase-9, Bax, and Bcl-2 were analyzed by Western blot. β-actin was used as a control. Data were normalized to the mean value of the control group and presented as the mean ± SD *<i>P</i> < 0.05, **<i>P</i> < 0.01 versus control. PCR: polymerase chain reaction; SD: Standard deviation; LCC: Large cell carcinoma; EE: <i>Euphorbiaceae</i> extract