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  Indian J Med Microbiol
 

Figure 4: The effects of KOTMIN13 on the MAPK phosphorylation. RAW 264.7 cells were pre-treated with different concentrations of KOTMIN13 for 1 h and then stimulated with LPS (200 ng/mL) for 30 min. Phosphorylation and total protein expression of MAPK were detected by Western blot analysis using specific antibodies. Data represent the means ± SEM of three independent experiments. Significantly different from the LPS-unstimulated value, ###P < 0.001. Significantly different from the LPS-stimulated value, *P < 0.05, **P < 0.01, and ***P < 0.001.

Figure 4: The effects of KOTMIN13 on the MAPK phosphorylation. RAW 264.7 cells were pre-treated with different concentrations of KOTMIN13 for 1 h and then stimulated with LPS (200 ng/mL) for 30 min. Phosphorylation and total protein expression of MAPK were detected by Western blot analysis using specific antibodies. Data represent the means ± SEM of three independent experiments. Significantly different from the LPS-unstimulated value, <sup>###</sup><i>P</i> < 0.001. Significantly different from the LPS-stimulated value, <sup>*</sup><i>P</i> < 0.05, <sup>**</sup><i>P</i> < 0.01, and <sup>***</sup><i>P</i> < 0.001.