Figure 4: The effects of KOTMIN13 on the MAPK phosphorylation. RAW 264.7 cells were pre-treated with different concentrations of KOTMIN13 for 1 h and then stimulated with LPS (200 ng/mL) for 30 min. Phosphorylation and total protein expression of MAPK were detected by Western blot analysis using specific antibodies. Data represent the means ± SEM of three independent experiments. Significantly different from the LPS-unstimulated value, ###P < 0.001. Significantly different from the LPS-stimulated value, *P < 0.05, **P < 0.01, and ***P < 0.001.