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  Indian J Med Microbiol
 

Figure 3: Effect of KOTMIN13 on the activation of NF-κB. (a-c) The levels of NF-κB p65 and IκBα/IKK proteins by Western blot analysis. (d) The binding activity of NF-κB to DNA. (e) Immunofluorescence analysis for detecting the localization of NF-κB p65. Magnification for images was 400x. Data represent the means ± SEM of three independent experiments. Significantly different from the LPS-unstimulated value, ##P < 0.001 and ###P < 0.001. Significantly different from the LPS-stimulated value, *P < 0.05, **P < 0.01, and ***P < 0.001. NC, negative control (DMSD); PC, positive control (LPS-treatment group).

Figure 3: Effect of KOTMIN13 on the activation of NF-κB. (a-c) The levels of NF-κB p65 and IκBα/IKK proteins by Western blot analysis. (d) The binding activity of NF-κB to DNA. (e) Immunofluorescence analysis for detecting the localization of NF-κB p65. Magnification for images was 400x. Data represent the means ± SEM of three independent experiments. Significantly different from the LPS-unstimulated value, <sup>##</sup><i>P</i> < 0.001 and <sup>###</sup><i>P</i> < 0.001. Significantly different from the LPS-stimulated value, <sup>*</sup><i>P</i> < 0.05, <sup>**</sup><i>P</i> < 0.01, and <sup>***</sup><i>P</i> < 0.001. NC, negative control (DMSD); PC, positive control (LPS-treatment group).