Figure 3: Effect of KOTMIN13 on the activation of NF-κB. (a-c) The levels of NF-κB p65 and IκBα/IKK proteins by Western blot analysis. (d) The binding activity of NF-κB to DNA. (e) Immunofluorescence analysis for detecting the localization of NF-κB p65. Magnification for images was 400x. Data represent the means ± SEM of three independent experiments. Significantly different from the LPS-unstimulated value, ##P < 0.001 and ###P < 0.001. Significantly different from the LPS-stimulated value, *P < 0.05, **P < 0.01, and ***P < 0.001. NC, negative control (DMSD); PC, positive control (LPS-treatment group).