Figure 1: The effects of KOTMIN13 on NO/PGE2 production and iNOS/COX-2 expression. (a and b) Secreted NO and PGE2 were measured in the culture media by Griess reagents and an EIA kit, respectively. (c and d) Protein levels of iNOS and COX-2 were evaluated with Western blot analysis. Data represent the means ± SEM of three independent experiments. Significantly different from the LPS-unstimulated value, ###P < 0.001. Significantly different from the LPS-stimulated value, *P < 0.05, **P < 0.01, and ***P < 0.001. The ratio of intensity between iNOS or COX-2 and GAPDH was calculated. Dex, dexamethasone.