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  Indian J Med Microbiol
 

Figure 1: Morphological changes related to apoptosis observed under fluorescent microscope and DNA fragmentation analysis. [A] HepG2 cells treated with either the hexane or the chloroform extract for 24 h stained with acridine orange/ethidium bromide (AO/EB). A Control cells; B, D, F cells treated with 10, 100, 400 μg/mL of the hexane extract, respectively. C, E, G cells treated with 10, 100, 400 μg/mL of the chloroform extract, respectively. [B] DNA fragmentation in HepG2 cells following treatment with hexane and chloroform extracts analyzed by agarose gel electrophoresis.

Figure 1: Morphological changes related to apoptosis observed under fluorescent microscope and DNA fragmentation analysis. [A] HepG2 cells treated with either the hexane or the chloroform extract for 24 h stained with acridine orange/ethidium bromide (AO/EB). A Control cells; B, D, F cells treated with 10, 100, 400 μg/mL of the hexane extract, respectively. C, E, G cells treated with 10, 100, 400 μg/mL of the chloroform extract, respectively. [B] DNA fragmentation in HepG2 cells following treatment with hexane and chloroform extracts analyzed by agarose gel electrophoresis.