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  Indian J Med Microbiol
 

Figure 4: Effect of the sponge extract on apoptosis of MCF-7 cells. Apoptotic cells were stained by Annexin-V and detected by flow cytometry. Concentration-dependent increase in percentage of apoptotic cells was found: (A) Control (0 µg/ml), (B) 25 µg/ml, (C) 50 µg/ml, (D) 100 µg/ml of sponge extract. (E) The histogram shows the mean Annexin-V positive MCF-7 cells (mean ± SD) of three experiments. ANOVA followed by Dunnett's posttest was performed to find out significant difference among treatments. ***P ≤ 0.001. ****P ≤ 0.0001

Figure 4: Effect of the sponge extract on apoptosis of MCF-7 cells. Apoptotic cells were stained by Annexin-V and detected by flow cytometry. Concentration-dependent increase in percentage of apoptotic cells was found: (A) Control (0 µg/ml), (B) 25 µg/ml, (C) 50 µg/ml, (D) 100 µg/ml of sponge extract. (E) The histogram shows the mean Annexin-V positive MCF-7 cells (mean ± SD) of three experiments. ANOVA followed by Dunnett's posttest was performed to find out significant difference among treatments. <sup>***</sup><i>P</i> ≤ 0.001. <sup>****</sup><i>P</i> ≤ 0.0001