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  Indian J Med Microbiol
 

Figure 2: Morphological assessment of apoptosis and necrosis in HepG2 cells stained with acridine orange and ethidium bromide (AO/EB). (2A) The leaves and root and extracts (IC50 concentration) induced apoptotic morphological features in HepG2 cells after 24 h and 48 h treatment. [Figure 2]b and c depict the percentage of normal, apoptotic and necrotic cells after treatment with leaves and root extracts (IC50 concentration) at 24 h and 48 h respectively. The values were expressed as mean ± SD of three independent experiments (n = 3). Untreated cells were used as control for comparison. Statistical comparison was made using one-way ANOVA followed by Bonferroni (* denotes p < 0.05). Error bar represents standard deviation in triplicates

Figure 2: Morphological assessment of apoptosis and necrosis in HepG2 cells stained with acridine orange and ethidium bromide (AO/EB). (2A) The leaves and root and extracts (IC<sub>50</sub> concentration) induced apoptotic morphological features in HepG2 cells after 24 h and 48 h treatment. [Figure 2]b and c depict the percentage of normal, apoptotic and necrotic cells after treatment with leaves and root extracts (IC<sub>50</sub> concentration) at 24 h and 48 h respectively. The values were expressed as mean ± SD of three independent experiments (n = 3). Untreated cells were used as control for comparison. Statistical comparison was made using one-way ANOVA followed by Bonferroni (<sup>*</sup> denotes p < 0.05). Error bar represents standard deviation in triplicates