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  Indian J Med Microbiol
 

Figure 2: Effects of total saponins from Dioscorea nipponica Makino on inhibition of nuclear factor-κB p65 activity. (a) Shifted band of nuclear factor-κB p65 DNA complexes. Lane 1: positive control, lane 2: negative control, lane 3: cold competition assay, lane 4: 1 h after the experiment, lane 5: 1 h after inducing, lane 6: 1 h after treating, lane 7, 24 h after the experiment, lane 8: 24 h after inducing, lane 9: 24 h after treating; lane 10, 48 h after the experiment, lane 11: 48 h after inducing, lane 12: 48 h after treating, lane 13: 72 h after the experiment, lane 14: 72 h after inducing, lane 15: 72 h after treating. (b) Amounts of nuclear factor-κB p65 DNA complexes. The data represent the values of the mean ± standard error of the mean for five treatments. #P < 0.05, ##P < 0.01, versus normal group (independent samples t-test); *P < 0.05, **P < 0.01, versus model group (independent samples t-test)

Figure 2: Effects of total saponins from <i>Dioscorea nipponica</i> Makino on inhibition of nuclear factor-κB p65 activity. (a) Shifted band of nuclear factor-κB p65 DNA complexes. Lane 1: positive control, lane 2: negative control, lane 3: cold competition assay, lane 4: 1 h after the experiment, lane 5: 1 h after inducing, lane 6: 1 h after treating, lane 7, 24 h after the experiment, lane 8: 24 h after inducing, lane 9: 24 h after treating; lane 10, 48 h after the experiment, lane 11: 48 h after inducing, lane 12: 48 h after treating, lane 13: 72 h after the experiment, lane 14: 72 h after inducing, lane 15: 72 h after treating. (b) Amounts of nuclear factor-κB p65 DNA complexes. The data represent the values of the mean ± standard error of the mean for five treatments. #<i>P</i> < 0.05, ##<i>P</i> < 0.01, versus normal group (independent samples <i>t</i>-test); *<i>P</i> < 0.05, **<i>P</i> < 0.01, versus model group (independent samples <i>t</i>-test)