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  Indian J Med Microbiol
 

Figure 8: Western blot analysis for oxidant stress and apoptosis-related protein expression in RAW264.7 cells. (A) RAW264.7 cells were treated with lipopolysaccharide in the presence or absence of mesalazine or baicalin, and protein expressions were analyzed by western blotting analysis. a: 1 μg/ml BSA; b: 1 μmL lipopolysaccharide; c: Mesalazine + lipopolysaccharide; d: Baicalin (1.0 × 10-5 M) + lipopolysaccharide; e: Baicalin (5.0 × 10-6 M) + lipopolysaccharide; f: Baicalin (1.0 × 10-6 M) + lipopolysaccharide. (B) transforming growth factor beta-1 protein level; (C) caspase-3 protein level; (D) cleaved caspase-3 protein level; (E) caspase-9 protein level; (F) cleaved caspase-9 protein level; (G) Bax protein level; (H) Bcl-2 protein level; (I) Fas protein level; (J) FasL protein level. Data are expressed as means ± standard deviation, n = 3. &&P < 0.01, lipopolysaccharide versus BSA group; **P < 0.01, mesalazine versus lipopolysaccharide group; #P < 0.05, ##P < 0.01, baicalin versus lipopolysaccharide group

Figure 8: Western blot analysis for oxidant stress and apoptosis-related protein expression in RAW264.7 cells. (A) RAW264.7 cells were treated with lipopolysaccharide in the presence or absence of mesalazine or baicalin, and protein expressions were analyzed by western blotting analysis. a: 1 μg/ml BSA; b: 1 μmL lipopolysaccharide; c: Mesalazine + lipopolysaccharide; d: Baicalin (1.0 × 10<sup>-5</sup> M) + lipopolysaccharide; e: Baicalin (5.0 × 10<sup>-6</sup> M) + lipopolysaccharide; f: Baicalin (1.0 × 10<sup>-6</sup> M) + lipopolysaccharide. (B) transforming growth factor beta-1 protein level; (C) caspase-3 protein level; (D) cleaved caspase-3 protein level; (E) caspase-9 protein level; (F) cleaved caspase-9 protein level; (G) Bax protein level; (H) Bcl-2 protein level; (I) Fas protein level; (J) FasL protein level. Data are expressed as means ± standard deviation, <i>n</i> = 3. &&<i>P</i> < 0.01, lipopolysaccharide versus BSA group; **<i>P</i> < 0.01, mesalazine versus lipopolysaccharide group; #<i>P</i> < 0.05, ##<i>P</i> < 0.01, baicalin versus lipopolysaccharide group