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  Indian J Med Microbiol
 

Figure 4: Effect of Thymelaea microphylla acetone extract on tumor necrosis factor-α induced mRNA expression of the adhesion molecule E-selectin, in human umbilical vein endothelial cells. Cells were pretreated with Thymelaea microphylla extract (20–40 μg/ml) for 24 h and then exposed for 2 h to tumor necrosis factor-α (20 ng/ml). Cultures treated with the vehicle alone (dimethyl sulfoxide 0.1%) were used as controls. Values are expressed as 2-ΔΔCt normalized to control and reported as mean ± standard deviation of three experiments. *P < 0.05 versus respective tumor necrosis factor-α 0 ng/ml; §P < 0.05 versus vehicle exposed to tumor necrosis factor-α 20 ng/ml

Figure 4: Effect of <i>Thymelaea microphylla</i> acetone extract on tumor necrosis factor-α induced mRNA expression of the adhesion molecule E-selectin, in human umbilical vein endothelial cells. Cells were pretreated with <i>Thymelaea microphylla</i> extract (20–40 μg/ml) for 24 h and then exposed for 2 h to tumor necrosis factor-α (20 ng/ml). Cultures treated with the vehicle alone (dimethyl sulfoxide 0.1%) were used as controls. Values are expressed as 2<sup>-ΔΔCt</sup> normalized to control and reported as mean ± standard deviation of three experiments. *<i>P</i> < 0.05 versus respective tumor necrosis factor-α 0 ng/ml; §<i>P</i> < 0.05 versus vehicle exposed to tumor necrosis factor-α 20 ng/ml