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  Indian J Med Microbiol
 

Figure 3: Fluorescent micrograph of acridine orange and propidium iodide double-stained cells. Cells were treated at IC50 of khat extract with and without acetyl cysteine N-acetyl cysteine at time-dependent manner. (a) Untreated cells showed normal structure without prominent apoptosis. (b) Early apoptosis features were seen after 24 h representing intercalated acridine orange (bright green). (c) Blebbing and nuclear margination were noticed in 48 h treatment. (d) Late apoptosis was seen in 72 h incubated cells. (e) There was a significant (P < 0.05) difference in the cell populations among the different criteria

Figure 3: Fluorescent micrograph of acridine orange and propidium iodide double-stained cells. Cells were treated at IC<sub>50</sub> of khat extract with and without acetyl cysteine N-acetyl cysteine at time-dependent manner. (a) Untreated cells showed normal structure without prominent apoptosis. (b) Early apoptosis features were seen after 24 h representing intercalated acridine orange (bright green). (c) Blebbing and nuclear margination were noticed in 48 h treatment. (d) Late apoptosis was seen in 72 h incubated cells. (e) There was a significant (<i>P</i> < 0.05) difference in the cell populations among the different criteria