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  Indian J Med Microbiol
 

Figure 1: Thin layer chromatography, thin layer chromatography-2,2-diphenyl-1-picryl hydrazyl and high-performance thin layer chromatography analysis. (a) Comparative thin layer chromatography profile of rutin, crude alcoholic extract and ethyl acetate fraction after derivatization with 1% natural product reagent under ultraviolet light at 366 nm, lane 1 -rutin, lane 2 -crude alcoholic extract, lane 3 -ethyl acetate fraction, lane 4 -water fraction; (b) comparative thin layer chromatography-2,2-diphenyl-1-picryl hydrazyl profile of rutin, crude alcoholic extract and ethyl acetate fraction after derivatizing with 0.1% 2,2-diphenyl-1-picryl hydrazyl under visible light, lane 1 -rutin, lane 2 -crude alcoholic extract, lane 3 -ethyl acetate fraction, lane 4 -water fraction; (c) high-performance thin layer chromatography profile of ethyl acetate fraction; (d) high-performance thin layer chromatography densitogram of ethyl acetate fraction at 420 nm

Figure 1: Thin layer chromatography, thin layer chromatography-2,2-diphenyl-1-picryl hydrazyl and high-performance thin layer chromatography analysis. (a) Comparative thin layer chromatography profile of rutin, crude alcoholic extract and ethyl acetate fraction after derivatization with 1% natural product reagent under ultraviolet light at 366 nm, lane 1 -rutin, lane 2 -crude alcoholic extract, lane 3 -ethyl acetate fraction, lane 4 -water fraction; (b) comparative thin layer chromatography-2,2-diphenyl-1-picryl hydrazyl profile of rutin, crude alcoholic extract and ethyl acetate fraction after derivatizing with 0.1% 2,2-diphenyl-1-picryl hydrazyl under visible light, lane 1 -rutin, lane 2 -crude alcoholic extract, lane 3 -ethyl acetate fraction, lane 4 -water fraction; (c) high-performance thin layer chromatography profile of ethyl acetate fraction; (d) high-performance thin layer chromatography densitogram of ethyl acetate fraction at 420 nm