Close
  Indian J Med Microbiol
 

Figure 1: Thin layer chromatography of extract at various extraction stages. (a) Derivatized with acidified 1% 4-dimethylaminocinnamaldehyde. Lane 1: Extraction cycle 1, Lane 2: Extraction cycle 2, Lane 3: Extraction cycle 3, Lane 4: Pooled crude extract, Lane 5: Aqueous fraction, Lane 6: Ethyl acetate fraction and Lane 7: (+)-catechin. (b) Thin layer chromatography bioautography of cocoa extracts with 0.1% diphenylpicrylhydrazyl. Lane 1: Pooled extract, Lane 2: Ethyl acetate extract, Lane 3: Water fraction of extract. (c) Ultraviolet spectra analysis of standards and extracts after high-performance thin layer chromatography. (i) (+)-catechin, (ii) procyanidin B2 and (iii) ethyl acetate extract of cocoa powder

Figure 1: Thin layer chromatography of extract at various extraction stages. (a) Derivatized with acidified 1% 4-dimethylaminocinnamaldehyde. Lane 1: Extraction cycle 1, Lane 2: Extraction cycle 2, Lane 3: Extraction cycle 3, Lane 4: Pooled crude extract, Lane 5: Aqueous fraction, Lane 6: Ethyl acetate fraction and Lane 7: (+)-catechin. (b) Thin layer chromatography bioautography of cocoa extracts with 0.1% diphenylpicrylhydrazyl. Lane 1: Pooled extract, Lane 2: Ethyl acetate extract, Lane 3: Water fraction of extract. (c) Ultraviolet spectra analysis of standards and extracts after high-performance thin layer chromatography. (i) (+)-catechin, (ii) procyanidin B2 and (iii) ethyl acetate extract of cocoa powder