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  Indian J Med Microbiol
 

Figure 4: Cucumis melo ssp. agrestis var. agrestis fractions inhibited adipogenesis, lipogenesis and increased lipid metabolism and reverse cholesterol transport. Mouse 3T3-L1 preadipocytes were differentiated into adipocytes with or without presence of various concentrations of Cucumis melo ssp. agrestis var. agrestis fractions (Cucumis melo ssp. agrestis var. agrestis water fraction and Cucumis melo ssp. agrestis var. agrestis hexane fraction at 25, 50 100 μg/ml concentration). Intracellular neutral lipids were stained with oil-red-O and absorbance was measured at 492 nm. (a) Oil-red-O staining in 3T3-L1 adipocytes. Syrian golden hamsters kept on chow or high fat diet, were treated with fenofibrate (100 mg/kg) or Cucumis melo ssp. agrestis var. agrestis water fraction and Cucumis melo ssp. agrestis var. agrestis hexane fraction at 50 mg/kg for 7 days. At 8th day, after overnight fast, liver and epididymal white adipose tissue were collected in liquid nitrogen. (b) Protein expression of fatty acid synthase in epididymal white adipose tissue. (c) Protein expression of fatty acid synthase, ACC, ATP-citrate lyase in hepatic tissue (d) relative mRNA expression of LXR α. E. Relative mRNA of sterol regulatory element binding protein 1c. (f ) Relative mRNA expression of LPL. (g) Relative mRNA expression of apolipoprotein A1. (h) Relative mRNA expression of lecithin-cholesterol acyltransferase 10. Gene expressions are relative to 18S rRNA and normalized by high fat diet-fed subgroups. Values are means (n = 8), with their standard error of the mean represented by vertical bars. Mean values were significantly different from the high-fat diets diet fed animals (one-way analysis of variance): *P < 0·05, **P < 0·01, ***P < 0·001

Figure 4: <i>Cucumis melo ssp. agrestis var. agrestis</i> fractions inhibited adipogenesis, lipogenesis and increased lipid metabolism and reverse cholesterol transport. Mouse 3T3-L1 preadipocytes were differentiated into adipocytes with or without presence of various concentrations of <i>Cucumis melo ssp. agrestis var. agrestis</i> fractions (<i>Cucumis melo ssp. agrestis var. agrestis water fraction</i> and <i>Cucumis melo ssp. agrestis var. agrestis hexane fraction</i> at 25, 50 100 μg/ml concentration). Intracellular neutral lipids were stained with oil-red-O and absorbance was measured at 492 nm. (a) Oil-red-O staining in 3T3-L1 adipocytes. Syrian golden hamsters kept on chow or high fat diet, were treated with fenofibrate (100 mg/kg) or <i>Cucumis melo ssp. agrestis var. agrestis water fraction</i> and <i>Cucumis melo ssp. agrestis var. agrestis hexane fraction</i> at 50 mg/kg for 7 days. At 8th day, after overnight fast, liver and epididymal white adipose tissue were collected in liquid nitrogen. (b) Protein expression of fatty acid synthase in epididymal white adipose tissue. (c) Protein expression of fatty acid synthase, ACC, ATP-citrate lyase in hepatic tissue (d) relative mRNA expression of LXR α. E. Relative mRNA of sterol regulatory element binding protein 1c. (f ) Relative mRNA expression of LPL. (g) Relative mRNA expression of apolipoprotein A1. (h) Relative mRNA expression of lecithin-cholesterol acyltransferase 10. Gene expressions are relative to 18S rRNA and normalized by high fat diet-fed subgroups. Values are means (<i>n</i> = 8), with their standard error of the mean represented by vertical bars. Mean values were significantly different from the high-fat diets diet fed animals (one-way analysis of variance): *<i>P</i> < 0·05, **<i>P</i> < 0·01, ***<i>P</i> < 0·001