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  Indian J Med Microbiol
 

Figure 6: High performance thin layer chromatography chromatograms of: (a) Standard lupeol (Rf = 0.75), (b) Standard β-sitosterol (Rf = 0.55), (c) Ethanol extract of Wrightia tinctoria, (d) Petroleum ether fraction of Wrightia tinctoria, (e) Ethyl acetate fraction of Wrightia tinctoria. Rf value of 0.75 ± 0.02 indicates lupeol and 0.55 ± 0.02 indicates β-sitosterol. Mobile phase used was toluene: Methanol: Formic acid (7:2.7:0.3). Detection was carried out at 600 nm after spraying with anisaldehyde sulfuric acid reagent

Figure 6: High performance thin layer chromatography chromatograms of: (a) Standard lupeol (<i>R</i><sub>f</sub> = 0.75), (b) Standard β-sitosterol (<i>R</i><sub>f</sub> = 0.55), (c) Ethanol extract of <i>Wrightia tinctoria</i>, (d) Petroleum ether fraction of <i>Wrightia tinctoria</i>, (e) Ethyl acetate fraction of <i>Wrightia tinctoria. R</i><sub>f</sub> value of 0.75 ± 0.02 indicates lupeol and 0.55 ± 0.02 indicates β-sitosterol. Mobile phase used was toluene: Methanol: Formic acid (7:2.7:0.3). Detection was carried out at 600 nm after spraying with anisaldehyde sulfuric acid reagent