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  Indian J Med Microbiol
 

Figure 5: (a) Ethanol extract of Wrightia tinctoria, petroleum ether fraction of Wrightia tinctoria and ethyl acetate fraction of Wrightia tinctoria induce apoptosis in MCF-7 cells analyzed by acridine orange/ethidium bromide staining. Representative images of cells after treatment for 48 h. Viable cells appear green indicated by white arrows; apoptotic cells appear yellow/orange indicated by blue arrows. (b) Percent of apoptotic cells were determined by counting apoptotic cells in six different fields. Results are expressed as mean ± standard error of the mean. *P < 0.001 compared with negative control. Treatments were carried out in triplicate

Figure 5: (a) Ethanol extract of <i>Wrightia tinctoria</i>, petroleum ether fraction of <i>Wrightia tinctoria</i> and ethyl acetate fraction of <i>Wrightia tinctoria</i> induce apoptosis in MCF-7 cells analyzed by acridine orange/ethidium bromide staining. Representative images of cells after treatment for 48 h. Viable cells appear green indicated by white arrows; apoptotic cells appear yellow/orange indicated by blue arrows. (b) Percent of apoptotic cells were determined by counting apoptotic cells in six different fields. Results are expressed as mean ± standard error of the mean. *<i>P</i> < 0.001 compared with negative control. Treatments were carried out in triplicate